Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   V6985_RS02915 Genome accession   NZ_CP146071
Coordinates   592292..593401 (-) Length   369 a.a.
NCBI ID   WP_265433982.1    Uniprot ID   -
Organism   Aeromonas salmonicida strain GMT3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 587292..598401
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6985_RS02890 (V6985_02895) - 587431..587661 (+) 231 WP_021140462.1 hypothetical protein -
  V6985_RS02895 (V6985_02900) brnQ 587737..589050 (-) 1314 WP_257978485.1 branched-chain amino acid transport system II carrier protein -
  V6985_RS02900 (V6985_02905) - 589236..589946 (-) 711 WP_111588947.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  V6985_RS02905 (V6985_02910) srmB 590075..591298 (+) 1224 WP_005320462.1 ATP-dependent RNA helicase SrmB -
  V6985_RS02910 (V6985_02915) yaaA 591487..592260 (-) 774 WP_111588946.1 peroxide stress protein YaaA -
  V6985_RS02915 (V6985_02920) pilU 592292..593401 (-) 1110 WP_265433982.1 type IVa pilus ATPase TapU Machinery gene
  V6985_RS02920 (V6985_02925) pilT 593437..594471 (-) 1035 WP_005320466.1 type IVa pilus ATPase TapT Machinery gene
  V6985_RS02925 (V6985_02930) - 594511..595212 (+) 702 WP_059169416.1 YggS family pyridoxal phosphate-dependent enzyme -
  V6985_RS02930 (V6985_02935) proC 595303..596127 (+) 825 WP_409011670.1 pyrroline-5-carboxylate reductase -
  V6985_RS02935 (V6985_02940) - 596156..596707 (+) 552 WP_005320477.1 YggT family protein -
  V6985_RS02940 (V6985_02945) yggU 596707..597006 (+) 300 WP_409011671.1 DUF167 family protein YggU -
  V6985_RS02945 (V6985_02950) - 597025..597444 (+) 420 WP_409011672.1 DUF4426 domain-containing protein -
  V6985_RS02950 (V6985_02955) - 597513..597812 (-) 300 WP_059112460.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41117.14 Da        Isoelectric Point: 6.3717

>NTDB_id=941540 V6985_RS02915 WP_265433982.1 592292..593401(-) (pilU) [Aeromonas salmonicida strain GMT3]
MNLDALLAELVARKGSDLFITVGTPPTLKINGHLFSLGSETLDKQSALTLVRETLSPEHFERYIRTREGNYAIHREALGR
FRVSAFWQQELPGMVVRRIETRIPTFDELTLPPILQEVAMAKRGLVLFVGATGTGKSTTQAAMIGYRNQNADGHILTVED
PVEFVHQHGRSLITQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQLLFDLSFNLRAIVAQQLVPSLDGRRRFAAFEVLLNTPLITDIIRKGEVHRLKEVMTKSTELGMQTFDQA
LFTLFCAGQIGYSEALAHADSANDLRLLIKLSGREQMGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=941540 V6985_RS02915 WP_265433982.1 592292..593401(-) (pilU) [Aeromonas salmonicida strain GMT3]
ATGAATCTGGATGCTCTATTGGCCGAACTGGTCGCACGCAAAGGCTCGGATCTGTTCATCACGGTTGGTACGCCCCCCAC
CCTCAAGATCAACGGGCATCTGTTCTCCCTCGGCAGCGAAACGCTCGACAAGCAGAGCGCGCTGACCCTGGTCAGGGAGA
CGCTCAGCCCTGAGCATTTCGAGCGCTACATCCGCACCAGGGAGGGCAACTACGCCATCCACCGCGAGGCACTGGGACGC
TTTCGGGTGAGTGCCTTCTGGCAGCAGGAGCTGCCAGGCATGGTGGTGCGCCGTATAGAGACACGCATTCCAACCTTCGA
CGAACTGACCCTGCCGCCCATCTTGCAGGAGGTGGCCATGGCCAAACGCGGTCTGGTGCTGTTCGTCGGCGCCACCGGAA
CGGGCAAATCCACCACTCAGGCAGCCATGATCGGCTATCGAAACCAGAATGCGGATGGCCATATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGCAGCCTGATCACCCAGCGGGAAGTGGGCATAGATACGGAATCATTCGATGT
GGCGCTGAAGAGCTCCCTGCGCCAGGCACCTGACGTTATCCTCATCGGCGAGATCCGAAGCCAGGAAACCATGGAGTTCG
CACTGCAATTTGCCGAGACCGGCCATCTTTGCCTCGCGACCCTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTCCATCTGGTGCCGCAGGAGAAACACCGCCAGTTACTGTTCGATCTCTCCTTCAACCTGCGTGCCATCGTCGCCCAGCA
ACTGGTGCCAAGCCTGGATGGTCGCCGCCGCTTCGCTGCCTTTGAAGTGCTGCTCAACACGCCGCTCATCACGGATATCA
TCCGCAAAGGCGAGGTTCATCGTCTCAAGGAGGTGATGACCAAGTCCACCGAGCTTGGCATGCAGACCTTCGATCAGGCG
CTGTTTACCCTGTTTTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCCGGCCGTGAGCAGATGGGTGCAGGCACACTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

59.239

99.729

0.591
  pilU Pseudomonas stutzeri DSM 10701

59.544

95.122

0.566
  pilU Acinetobacter baylyi ADP1

57.79

95.664

0.553
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.296

89.702

0.379
  pilT Legionella pneumophila strain ERS1305867

39.942

92.954

0.371
  pilT Legionella pneumophila strain Lp02

39.942

92.954

0.371
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.12

90.515

0.363
  pilT Vibrio cholerae strain A1552

40.12

90.515

0.363