Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   V5M36_RS01625 Genome accession   NZ_CP145906
Coordinates   366697..368409 (-) Length   570 a.a.
NCBI ID   WP_338561337.1    Uniprot ID   -
Organism   Acinetobacter sp. KS-LM10     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 361697..373409
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V5M36_RS01600 (V5M36_01600) rlmB 362024..362773 (+) 750 WP_338561327.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  V5M36_RS01605 (V5M36_01605) - 362879..363802 (+) 924 WP_338561329.1 DMT family transporter -
  V5M36_RS01610 (V5M36_01610) coaE 363834..364454 (-) 621 WP_338561331.1 dephospho-CoA kinase -
  V5M36_RS01615 (V5M36_01615) pilD 364585..365445 (-) 861 WP_338561334.1 A24 family peptidase Machinery gene
  V5M36_RS01620 (V5M36_01620) pilC 365445..366671 (-) 1227 WP_338561335.1 type II secretion system F family protein Machinery gene
  V5M36_RS01625 (V5M36_01625) pilB 366697..368409 (-) 1713 WP_338561337.1 type IV-A pilus assembly ATPase PilB Machinery gene
  V5M36_RS01630 (V5M36_01630) tpiA 368673..369464 (+) 792 WP_338561339.1 triose-phosphate isomerase -
  V5M36_RS01635 (V5M36_01635) secG 369478..369807 (+) 330 WP_338561341.1 preprotein translocase subunit SecG -
  V5M36_RS01670 (V5M36_01670) rimP 371070..371594 (+) 525 WP_338561343.1 ribosome maturation factor RimP -
  V5M36_RS01675 (V5M36_01675) nusA 371635..373119 (+) 1485 WP_338561345.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63132.68 Da        Isoelectric Point: 5.5856

>NTDB_id=941057 V5M36_RS01625 WP_338561337.1 366697..368409(-) (pilB) [Acinetobacter sp. KS-LM10]
MSLEQTAPKFSGISRRLVSEGILSAQQMQSAVLNAKKNHQQLIPFLVEHLHIKSSIIAELVAQEFGEPFFDLDAYDTNLL
PKDIVATNLITKHQILPLFQRGITLFVATSDPTNIEALEAIRFNSKLTVVPITVELDKLERLIDRTFANSDDFNFDSEDL
DVDIIATDPNQTNDDDNNAPGDDSPIVKYINKLLVDAIRMGASDLHFEPYEKSYRVRYRIDGVLRLIANPPLKLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNALPTLFGEKIVLRILDPTSAMLGIDALGYEEDQKALFMEALEKP
QGMLLITGPTGSGKTVSLYTGLNILNTENSNISTAEDPVEINLDGINQVNVNTKVGLTFSLALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSINLVIAQRLARRLCNHCKAPIDVPKKSLLELGF
TENDLANPDLTIFQPVGCSECREGYKGRLGIYEVMKVTPEISKIIMEDGNALDIAAASQRQGVKNLRRSGLIKVMKGLTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=941057 V5M36_RS01625 WP_338561337.1 366697..368409(-) (pilB) [Acinetobacter sp. KS-LM10]
ATGAGTTTAGAGCAAACTGCACCAAAATTTTCAGGGATCAGTCGTCGCTTAGTGAGTGAAGGGATTCTCAGTGCTCAACA
AATGCAGTCTGCAGTTCTAAATGCAAAAAAAAACCATCAACAACTTATCCCGTTTTTAGTCGAACATTTGCATATTAAAT
CGAGCATCATTGCAGAACTGGTGGCTCAAGAGTTTGGCGAACCTTTTTTTGATCTAGATGCCTATGATACGAACTTATTA
CCTAAAGATATCGTCGCTACAAACCTGATCACTAAGCATCAAATTCTCCCGCTGTTTCAACGTGGTATTACACTTTTTGT
TGCCACCAGCGATCCAACAAATATCGAAGCCCTCGAAGCGATACGCTTTAACAGTAAGCTTACAGTTGTTCCCATCACGG
TCGAATTGGACAAACTCGAACGTCTTATTGATAGAACCTTTGCGAATAGCGACGATTTTAATTTTGACAGCGAAGACTTA
GATGTCGATATCATCGCTACAGACCCCAATCAAACTAACGATGATGACAATAACGCGCCAGGTGATGACTCCCCTATCGT
TAAATATATTAATAAGTTATTGGTCGATGCAATTCGCATGGGCGCATCAGATTTGCATTTTGAGCCTTATGAAAAAAGCT
ACCGTGTACGCTACCGTATCGATGGCGTTTTAAGACTGATCGCAAACCCACCTTTAAAACTTGCCAATCGTTTGGCATCA
CGTCTTAAAGTCATGTCACAAATGGATATTTCTGAAAAACGTGTCCCGCAAGATGGGCGAATTAAATTAAAACTTTCAAA
ATCCAAAGCCATTGATTTCCGTGTAAATGCTTTACCCACACTGTTTGGTGAAAAAATAGTCCTACGTATACTTGATCCCA
CCAGTGCCATGCTCGGTATTGATGCTTTGGGTTATGAAGAAGATCAAAAAGCATTGTTTATGGAAGCCCTTGAAAAGCCA
CAGGGCATGCTTTTAATCACAGGGCCAACAGGCTCTGGTAAAACTGTATCGCTCTATACTGGTTTAAATATTTTAAATAC
TGAAAACTCGAATATTTCTACTGCCGAAGATCCTGTCGAAATTAATTTAGATGGCATTAATCAAGTCAACGTCAACACCA
AAGTCGGGCTTACTTTTTCGCTCGCGCTTAAATCATTCTTACGTCAAGATCCAGATATTATTATGGTCGGTGAGATTCGT
GACTTAGAAACTGCTGAAATTGCCATCAAAGCAGCGCAAACAGGTCATATGGTGATGTCAACCTTGCATACTAACAGCGC
GCCTGAAACACTGACAAGATTAAGAAATATGGGAGTACCTTCTTTCAATATTGCCACATCGATCAATCTCGTGATTGCTC
AACGGTTGGCGCGCCGTTTATGTAATCATTGTAAAGCGCCGATTGATGTTCCTAAAAAAAGCTTACTTGAATTAGGTTTC
ACTGAAAATGATCTGGCTAATCCTGACCTAACTATTTTTCAACCTGTGGGCTGTTCTGAATGTAGAGAAGGATATAAAGG
TCGTCTGGGAATTTATGAAGTGATGAAAGTCACACCAGAAATTTCAAAAATTATCATGGAAGATGGAAATGCACTTGATA
TTGCAGCTGCTTCTCAAAGGCAAGGAGTCAAAAATCTCCGTCGCTCTGGATTGATTAAAGTCATGAAAGGTTTAACTTCA
CTGCAAGAAGTTAATCGCGTAACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-150)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

76.667

100

0.767
  pilB Acinetobacter baylyi ADP1

74.256

100

0.744
  pilB Legionella pneumophila strain ERS1305867

52.091

100

0.525
  pilB Vibrio parahaemolyticus RIMD 2210633

48.944

99.649

0.488
  pilF Neisseria gonorrhoeae MS11

48.577

98.596

0.479
  pilB Vibrio campbellii strain DS40M4

47.887

99.649

0.477
  pilB Vibrio cholerae strain A1552

48.825

97.018

0.474
  pilB/pilB1 Synechocystis sp. PCC 6803

34.41

100

0.374
  pilF Thermus thermophilus HB27

36.174

100

0.365