Detailed information NTG0934801

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: V3H56_RS16870
• Genome accession: NZ_CP144763
• Coordinates: 3862845..3863960 (-)
• Length: 371 a.a.
• NCBI ID: WP_042732331.1
• Uniprot ID: A0A0D0P3H5
• Organism: Pseudomonas sp. MS646
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3857845..3868960

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V3H56_RS16855 (V3H56_16855)	-	3858378..3859769 (+)	1392	WP_042732360.1	GntP family permease	-
V3H56_RS16860 (V3H56_16860)	-	3859777..3860553 (+)	777	WP_042732361.1	3-hydroxybutyrate dehydrogenase	-
V3H56_RS16865 (V3H56_16865)	-	3860763..3862718 (+)	1956	WP_123579854.1	acetoacetate--CoA ligase	-
V3H56_RS16870 (V3H56_16870)	pilU	3862845..3863960 (-)	1116	WP_042732331.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
V3H56_RS16875 (V3H56_16875)	-	3864093..3866630 (+)	2538	WP_338473641.1	PAS domain-containing protein	-
V3H56_RS16880 (V3H56_16880)	-	3866676..3866951 (+)	276	WP_019690952.1	peptidylprolyl isomerase	-
V3H56_RS16885 (V3H56_16885)	-	3867073..3867990 (-)	918	WP_042731869.1	sugar kinase	-

Sequence

Protein

• Length: 371 a.a.
• Molecular weight: 40973.87 Da
• Isoelectric Point: 6.0174

>NTDB_id=934801 V3H56_RS16870 WP_042732331.1 3862845..3863960(-) (pilU) [Pseudomonas sp. MS646]
MEIDPLLRILASQDGSDLYLSTGAPPCAKFEGVLKPLGSQAFKVGDIAGLAESLMDAEQRLEFDRELEMNLAISLAGVGR
FRVNVFKQRNDVSMVIRNVKLDIPRFEDLKLPPVLLESVMRKQGLILFVGATGSGKSTSLAALIDYRNRHSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNANQALDRV
INFFPEERRPQLLSDLANNLQAFISQRLVRTVSGQRRAAVEVMLGSPTVADLIRRNELSELKGIMEKSEELGMQTFDQAL
FNLVVEGAIDEEQALKNADSANNLRLRLKLHAESGAAPPADPAAGEWGLVD

Nucleotide

• Length: 1116 bp

>NTDB_id=934801 V3H56_RS16870 WP_042732331.1 3862845..3863960(-) (pilU) [Pseudomonas sp. MS646]
ATGGAAATCGATCCCTTGTTGCGAATCCTGGCGAGCCAGGATGGCTCCGACCTTTATCTGTCCACCGGTGCACCGCCGTG
CGCGAAATTCGAGGGGGTGCTCAAGCCTCTGGGTTCCCAGGCGTTCAAGGTTGGCGACATTGCCGGGCTCGCCGAGTCTT
TGATGGACGCCGAACAGCGCCTTGAGTTCGATCGAGAGCTGGAAATGAACCTGGCAATTTCCCTCGCCGGCGTCGGGCGG
TTCCGGGTCAACGTCTTCAAGCAGCGTAACGACGTGTCCATGGTGATCCGCAACGTCAAGCTGGACATCCCGCGCTTCGA
AGACCTGAAGCTGCCTCCGGTGCTGCTGGAGAGCGTGATGCGCAAACAGGGGCTGATCCTGTTCGTCGGCGCCACGGGGT
CCGGCAAGTCGACGTCCCTGGCGGCGTTGATCGATTACCGCAATCGCCACAGCAGTGGCCACATCATCACCATCGAGGAC
CCGGTGGAGTATATCCATCGGCACAAAAAATCGATCATCAACCAGCGTGAGGTCGGTGTCGACACCCGCAGCTTTCACGC
CGCCCTGAAAAATACCCTGCGCCAGGCGCCGGATGTGGTGCTGATCGGTGAAATCCGCGACCGGGAAACCATGGAACACG
CCCTGGCCTTCGCCGACACCGGGCACCTGGTGATTTCCACGTTGCATGCCCATAACGCCAACCAGGCCCTGGACCGGGTG
ATCAATTTCTTTCCCGAAGAGCGCCGGCCGCAACTGCTCAGCGACCTGGCCAACAACCTCCAGGCCTTCATCTCCCAGCG
CCTGGTGCGCACCGTGAGCGGCCAGCGCCGCGCGGCGGTGGAGGTGATGCTGGGCTCGCCCACGGTGGCCGACCTGATCC
GGCGCAACGAACTGAGCGAGCTCAAGGGCATCATGGAGAAATCGGAAGAGCTGGGCATGCAAACCTTCGATCAGGCCCTG
TTCAACCTGGTGGTGGAAGGTGCCATCGACGAGGAGCAGGCGCTGAAGAATGCCGACTCGGCGAACAACCTGCGCTTGCG
CCTGAAGCTGCATGCCGAGTCGGGCGCCGCGCCGCCGGCTGATCCGGCGGCGGGGGAGTGGGGGTTGGTGGATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (80-271)

3D structure

• AlphaFold DB: A0A0D0P3H5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.056	95.687	0.536
pilU	Acinetobacter baylyi ADP1	53.168	97.844	0.52
pilU	Vibrio cholerae strain A1552	52.857	94.34	0.499
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.965	98.922	0.385
pilT	Pseudomonas stutzeri DSM 10701	40.407	92.722	0.375
pilT	Vibrio cholerae strain A1552	41.441	89.757	0.372
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.441	89.757	0.372
pilT	Pseudomonas aeruginosa PAK	39.826	92.722	0.369