Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   V4H42_RS06450 Genome accession   NZ_CP144454
Coordinates   1343425..1343859 (-) Length   144 a.a.
NCBI ID   WP_115173562.1    Uniprot ID   -
Organism   Pasteurella multocida strain PM1463     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1338425..1348859
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V4H42_RS06420 (V4H42_06420) - 1338989..1339258 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  V4H42_RS06425 (V4H42_06425) yacG 1339258..1339461 (-) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  V4H42_RS06430 (V4H42_06430) coaE 1339472..1340092 (-) 621 WP_005751078.1 dephospho-CoA kinase -
  V4H42_RS06435 (V4H42_06435) - 1340107..1340829 (-) 723 WP_115173559.1 A24 family peptidase -
  V4H42_RS06440 (V4H42_06440) pilC 1340829..1342040 (-) 1212 WP_338544147.1 type II secretion system F family protein Machinery gene
  V4H42_RS06445 (V4H42_06445) pilB 1342040..1343425 (-) 1386 WP_115173561.1 GspE/PulE family protein Machinery gene
  V4H42_RS06450 (V4H42_06450) pilA 1343425..1343859 (-) 435 WP_115173562.1 prepilin peptidase-dependent pilin Machinery gene
  V4H42_RS06455 (V4H42_06455) ampD 1344018..1344584 (+) 567 WP_115173563.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  V4H42_RS06460 (V4H42_06460) rppH 1345170..1345766 (+) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  V4H42_RS06465 (V4H42_06465) - 1345769..1346563 (+) 795 WP_338544150.1 sulfite exporter TauE/SafE family protein -
  V4H42_RS06470 (V4H42_06470) lgt 1346573..1347376 (+) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  V4H42_RS06475 (V4H42_06475) - 1347382..1348233 (+) 852 WP_005751072.1 thymidylate synthase -
  V4H42_RS06480 (V4H42_06480) tadA 1348248..1348748 (+) 501 WP_074865711.1 tRNA adenosine(34) deaminase TadA -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15118.58 Da        Isoelectric Point: 9.1281

>NTDB_id=933906 V4H42_RS06450 WP_115173562.1 1343425..1343859(-) (pilA) [Pasteurella multocida strain PM1463]
MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR
ADAAASKKYLKAITVKAGVITVTGQGSLEGISYTLTASGNAAQGVFWTVNCGAQSDIFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=933906 V4H42_RS06450 WP_115173562.1 1343425..1343859(-) (pilA) [Pasteurella multocida strain PM1463]
ATGAAAAAAGCTATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC
CTTATCGTGCCGAGGTAGAACTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT
GCTGATGCTGCAGCAAGTAAAAAATATTTAAAAGCCATCACGGTTAAAGCTGGGGTAATTACTGTTACTGGACAAGGGAG
CTTGGAAGGAATTAGCTATACGCTGACAGCGAGTGGTAACGCAGCTCAAGGTGTTTTTTGGACAGTTAATTGTGGTGCTC
AATCTGACATTTTCCCAGCAGGATTTTGTGCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

63.194

100

0.632
  pilA Haemophilus influenzae Rd KW20

61.111

100

0.611
  pilA Glaesserella parasuis strain SC1401

52.349

100

0.542
  pilA Vibrio cholerae C6706

36.735

100

0.375
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.735

100

0.375
  pilA Vibrio cholerae strain A1552

36.735

100

0.375
  pilA Vibrio campbellii strain DS40M4

35.333

100

0.368