Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Aeroheme_RS02360 Genome accession   NZ_CP144265
Coordinates   478666..480372 (-) Length   568 a.a.
NCBI ID   WP_411166624.1    Uniprot ID   -
Organism   Aeromonas sp. DSM 116730     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 473666..485372
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Aeroheme_RS02330 (Aeroheme_00471) mutT 474265..474669 (+) 405 WP_111911126.1 8-oxo-dGTP diphosphatase MutT -
  Aeroheme_RS02335 (Aeroheme_00472) yacG 474744..474938 (-) 195 WP_025328449.1 DNA gyrase inhibitor YacG -
  Aeroheme_RS02340 (Aeroheme_00473) zapD 474948..475670 (-) 723 WP_168235912.1 cell division protein ZapD -
  Aeroheme_RS02345 (Aeroheme_00474) coaE 475708..476322 (-) 615 WP_411166622.1 dephospho-CoA kinase -
  Aeroheme_RS02350 (Aeroheme_00475) pilD 476341..477213 (-) 873 WP_411166623.1 prepilin peptidase Machinery gene
  Aeroheme_RS02355 (Aeroheme_00476) pilC 477294..478535 (-) 1242 WP_106885656.1 type II secretion system F family protein Machinery gene
  Aeroheme_RS02360 (Aeroheme_00477) pilB 478666..480372 (-) 1707 WP_411166624.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  Aeroheme_RS02365 - 480376..480810 (-) 435 WP_395949385.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  Aeroheme_RS02370 (Aeroheme_00478) nadC 481123..481986 (-) 864 WP_411166625.1 carboxylating nicotinate-nucleotide diphosphorylase -
  Aeroheme_RS02375 (Aeroheme_00479) - 481990..482460 (-) 471 WP_171276940.1 TIGR02281 family clan AA aspartic protease -
  Aeroheme_RS02380 (Aeroheme_00480) ampD 482597..483169 (+) 573 WP_111911110.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  Aeroheme_RS02385 (Aeroheme_00481) pdhR 483445..484209 (+) 765 WP_106885661.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62549.92 Da        Isoelectric Point: 5.6705

>NTDB_id=933067 Aeroheme_RS02360 WP_411166624.1 478666..480372(-) (pilB) [Aeromonas sp. DSM 116730]
MTSSLNSGLATSLAASSLLSEPDSERYLSQARAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEESKLTAALGKLMDTEQDTLGMDHIDEAEI
SELEVSDEGSRLDESVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRSRSMDMRVNTLPTMWGEKIVIRLLDSSAARLNLDQLGFDDRQKAQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPDAELQELGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSDNIAKLIMQGANSLQIAAIAQKEGMRTLRISGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=933067 Aeroheme_RS02360 WP_411166624.1 478666..480372(-) (pilB) [Aeromonas sp. DSM 116730]
ATGACCTCTAGCCTCAATAGCGGCCTGGCGACCAGCCTGGCCGCCTCTTCTCTTCTCAGCGAGCCGGACTCAGAGCGATA
TCTGAGCCAGGCCAGGGCGCAGCGCAAGCCCTTTGTCACCTTCCTGATCGAGAACGACATCCTCGACAGCAAGGCGCTGG
CGGATTTCTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTTATCGAGAAACATCATGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
CCCGACCAATGTGTCGGCCCTGGAGGATTTCGGCTTCAGCTTCGGCCTGCACACTGAAGCCTTGCTGGTCGAGGAGAGCA
AGCTCACCGCCGCCCTCGGCAAGCTGATGGACACCGAGCAGGACACCCTGGGCATGGATCACATCGACGAGGCCGAGATC
TCGGAGCTCGAGGTGTCCGATGAGGGTTCGCGCCTCGACGAGAGCGTCAATACTGCCGACGACGATGCCCCCATAGTCAA
ATACATCCATAAAATCATGATGGATGCTATCAAGCGTGGCGCATCCGACCTGCATTTCGAACCCTATGAGACCAAGTACA
GGATCCGCTTCCGGATCGACGGCATACTGCACGAAATCGCCACCCCGCCGGTCAATCTGGCCAACCGTTTCGCGGCCCGT
CTCAAGGTGATGGCGAGACTCGATATCGCCGAGCGACGCCTGCCCCAGGATGGGCGGATCAAGCTGAAGATCTCACGCAG
CCGATCCATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGAGAGAAGATCGTCATCCGCTTGCTGGACTCGTCGG
CCGCCCGCCTCAACCTCGATCAGCTCGGCTTCGACGACCGCCAGAAGGCGCAATACCTGCATGCGCTCTCCAAGCCACAG
GGCATGATACTGGTCACGGGGCCCACGGGGTCGGGCAAGACGGTGTCGCTCTATACAGGTCTCAACATCCTCAATACCAG
CGAGGTCAACATCTCCACCGCCGAGGATCCGGTGGAGATCAACCTGCCCGGCGTCAATCAGGTGCAGATCAATCCCAAGG
CGGGGCTCACCTTCGCCAGCGCCCTGCGCTCCTTCCTGCGCCAGGATCCCGACATCGTCATGGTGGGGGAGATCCGGGAT
CTGGAAACCGCCGAGATTGCCATCAAGGCCGCCCAGACAGGTCACCTGGTACTATCAACCCTGCACACCAATTCGGCGGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTCCCCGCCTTCAACATCGCCTCCTCGGTGACCCTTATCATGGCCCAGC
GTCTCGCCCGCAAGCTGTGCGATCACTGCAAGGCGCCCGAGGTGGTGCCCGACGCCGAACTGCAGGAGCTGGGCTTTACC
CAGCAGCAGCTGGCCGCCGGACTGCGACTGTTCAAGCCGGTCGGCTGCAAGGAGTGTTCGGGAGGCTACAGAGGACGGGT
CGGCATCTATGAAATCATGCTGATGTCGGACAACATCGCCAAGCTGATCATGCAGGGGGCTAATTCGCTACAAATCGCCG
CCATAGCCCAGAAGGAGGGCATGCGCACCCTGCGCATCTCCGGCCTTGAGAAGGCCCGCCTGGGGGTCACCAGCCTGGCC
GAGATCAACAGGATCACCACGAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.85

99.824

0.548
  pilB Legionella pneumophila strain ERS1305867

54.594

99.648

0.544
  pilB Acinetobacter baylyi ADP1

54.982

98.944

0.544
  pilB Vibrio cholerae strain A1552

52.931

99.12

0.525
  pilB Vibrio campbellii strain DS40M4

50.707

99.648

0.505
  pilB Vibrio parahaemolyticus RIMD 2210633

49.911

99.12

0.495
  pilF Neisseria gonorrhoeae MS11

46.384

99.824

0.463
  pilF Thermus thermophilus HB27

39.305

96.303

0.379
  pilB/pilB1 Synechocystis sp. PCC 6803

38.267

97.535

0.373