Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   VX159_RS12975 Genome accession   NZ_CP144212
Coordinates   2848899..2850617 (+) Length   572 a.a.
NCBI ID   WP_371323307.1    Uniprot ID   -
Organism   Dechloromonas sp. ZY10     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2843899..2855617
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VX159_RS12955 (VX159_12940) - 2844583..2845194 (+) 612 WP_371323303.1 lytic transglycosylase domain-containing protein -
  VX159_RS12960 (VX159_12945) ffh 2845318..2846673 (-) 1356 WP_371323304.1 signal recognition particle protein -
  VX159_RS12965 (VX159_12950) - 2846703..2847542 (+) 840 WP_371323305.1 inner membrane protein YpjD -
  VX159_RS12970 (VX159_12955) - 2847543..2848820 (+) 1278 WP_371323306.1 HlyC/CorC family transporter -
  VX159_RS12975 (VX159_12960) pilB 2848899..2850617 (+) 1719 WP_371323307.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VX159_RS12980 (VX159_12965) pilC 2850633..2851862 (+) 1230 WP_371323308.1 type II secretion system F family protein Machinery gene
  VX159_RS12985 (VX159_12970) pilD 2851862..2852719 (+) 858 WP_371325523.1 A24 family peptidase Machinery gene
  VX159_RS12990 (VX159_12975) - 2852806..2853057 (+) 252 WP_371323309.1 FmdB family zinc ribbon protein -
  VX159_RS12995 (VX159_12980) - 2853057..2853695 (+) 639 WP_371325524.1 DUF502 domain-containing protein -
  VX159_RS13000 (VX159_12985) aspS 2853718..2855517 (+) 1800 WP_371323310.1 aspartate--tRNA ligase -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62248.33 Da        Isoelectric Point: 4.9231

>NTDB_id=932622 VX159_RS12975 WP_371323307.1 2848899..2850617(+) (pilB) [Dechloromonas sp. ZY10]
MAATPPHSTLSGLARALVQAGQLTEAEAEQLLQQASANKQTLAEQFLGSHSLPAADLARFIAETFGFPLLDLDAFDESLI
PIDAIDRKLIASHRVIPLNKRGNRLAVAMSDPTNLRALDEIRFQTGLAVDPVVVEANKLAPLVGKHAEAPQDALKTLAND
DLNLDLLDGAIENEPQEDTSNQDVDDAPIVRFIQKILLDAIEDGASDIHFEPYEKYYRIRFRVDGILREVASPPLAIKEK
LASRIKVISRLNIAEKRVPQDGRMRLVLSKNRAIDFRVSTLPTLQGEKIVLRILDPSSATLGIEALGYEPEQKAALLDAV
NRPYGMVLVTGPTGSGKTVSLYTCLNLLNKVGVNISTAEDPAEINLPGINQVNVDDKAGLTFSSALKAFLRQDPDIIMVG
EIRDLETAEIAIKAAQTGHLVLSTLHTNDAPQTLTRLMNMGIPTFNIASSILLITAQRLARRLCHCKQPMEVPEQSLLDA
GFRPEELDDSWTLYGPGGCERCKGTGYKGRVGIYQVMPISEDMQRLIMSGATAIDLANQAKQEGVKDLRESGLLKVKQGV
TSLDEVLSTTNA

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=932622 VX159_RS12975 WP_371323307.1 2848899..2850617(+) (pilB) [Dechloromonas sp. ZY10]
ATGGCAGCCACCCCACCCCATTCCACACTGAGCGGCCTGGCCCGCGCCCTTGTTCAGGCCGGCCAACTGACCGAAGCCGA
AGCCGAGCAATTACTGCAGCAAGCCAGTGCCAACAAGCAAACCCTGGCCGAGCAATTTCTCGGCTCGCACAGTTTGCCGG
CTGCCGACTTGGCCCGCTTCATCGCCGAAACATTTGGCTTTCCGCTACTCGACCTGGATGCGTTCGACGAGTCGCTGATT
CCGATTGACGCGATTGATCGCAAACTGATTGCCAGTCACCGGGTGATTCCGCTCAACAAGCGGGGGAACCGGCTGGCGGT
GGCCATGTCGGACCCGACCAATCTGAGAGCATTGGACGAAATCCGCTTTCAGACCGGACTGGCCGTAGACCCGGTGGTAG
TCGAAGCCAACAAATTGGCGCCACTGGTAGGCAAACACGCCGAAGCGCCTCAGGATGCACTCAAGACACTAGCCAACGAC
GACCTCAACCTGGACCTGCTCGATGGCGCCATCGAAAACGAACCACAGGAAGATACCAGCAACCAGGATGTCGATGACGC
ACCGATTGTTCGCTTCATCCAGAAAATCCTGCTCGATGCGATTGAGGACGGCGCCTCGGATATCCATTTCGAGCCCTACG
AAAAGTACTACCGCATCCGTTTCCGGGTAGACGGCATCTTGCGCGAAGTGGCAAGCCCCCCATTGGCGATCAAGGAAAAG
CTGGCCTCCCGGATCAAGGTCATCTCGCGCCTCAACATTGCTGAAAAGCGGGTTCCGCAGGATGGCCGGATGCGCCTCGT
ACTCTCGAAAAACCGTGCCATCGATTTCCGGGTCAGCACCCTGCCCACGCTGCAAGGCGAAAAGATAGTCCTGCGGATTC
TCGATCCCAGCTCAGCCACTCTCGGCATCGAAGCCCTGGGTTACGAACCGGAGCAAAAAGCGGCCTTGCTTGACGCGGTC
AACCGCCCCTACGGCATGGTTTTGGTCACCGGACCGACCGGTTCGGGCAAAACGGTATCGCTGTACACCTGCCTCAACCT
GCTGAACAAAGTTGGAGTCAATATCTCCACTGCCGAAGATCCGGCGGAAATCAACTTACCGGGAATCAATCAGGTCAACG
TCGACGACAAGGCCGGGCTCACCTTTTCCTCGGCGCTGAAGGCATTCCTCCGCCAGGATCCGGACATCATCATGGTCGGC
GAAATCCGCGATCTGGAAACGGCGGAAATCGCGATCAAGGCCGCGCAGACCGGACACCTGGTACTCTCGACGCTGCACAC
CAACGATGCGCCGCAGACACTGACCCGGCTGATGAACATGGGCATCCCGACCTTCAACATCGCCTCCAGCATCCTGCTGA
TCACCGCCCAGCGCCTGGCGCGGCGTCTGTGTCACTGCAAACAACCGATGGAAGTGCCGGAGCAGAGTCTGCTCGACGCC
GGCTTCCGGCCGGAGGAATTGGACGATTCCTGGACCCTGTACGGACCTGGCGGCTGCGAGCGTTGCAAAGGCACCGGCTA
CAAGGGACGGGTTGGGATCTATCAAGTCATGCCAATCAGCGAGGACATGCAACGCCTGATCATGAGCGGTGCCACCGCCA
TCGATCTGGCCAATCAGGCCAAGCAGGAAGGCGTCAAGGACTTGCGCGAATCAGGCTTGCTCAAAGTCAAGCAGGGAGTC
ACTTCGCTCGACGAAGTGCTGAGTACGACCAACGCCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(195-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.226

100

0.554
  pilB Acinetobacter baumannii D1279779

54.704

100

0.549
  pilF Neisseria gonorrhoeae MS11

53.534

98.951

0.53
  pilB Legionella pneumophila strain ERS1305867

52.456

99.65

0.523
  pilB Vibrio parahaemolyticus RIMD 2210633

49.47

98.951

0.49
  pilB Vibrio campbellii strain DS40M4

49.468

98.601

0.488
  pilB Vibrio cholerae strain A1552

49.293

98.951

0.488
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.437

99.301

0.392
  pilF Thermus thermophilus HB27

39.016

99.476

0.388
  pilB/pilB1 Synechocystis sp. PCC 6803

40.866

92.832

0.379