Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   VXF94_RS18490 Genome accession   NZ_CP143956
Coordinates   3581179..3581793 (+) Length   204 a.a.
NCBI ID   WP_014470701.1    Uniprot ID   A0AAP7N5G8
Organism   Bacillus amyloliquefaciens strain Fad 108     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3576179..3586793
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VXF94_RS18455 (VXF94_18455) aroE 3576866..3577708 (+) 843 WP_014470708.1 shikimate dehydrogenase -
  VXF94_RS18460 (VXF94_18460) yhbY 3577702..3577992 (+) 291 WP_014470707.1 ribosome assembly RNA-binding protein YhbY -
  VXF94_RS18465 (VXF94_18465) - 3578003..3578572 (+) 570 WP_014470706.1 nicotinate-nucleotide adenylyltransferase -
  VXF94_RS18470 (VXF94_18470) yqeK 3578562..3579122 (+) 561 WP_014470705.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  VXF94_RS18475 (VXF94_18475) rsfS 3579139..3579495 (+) 357 WP_014470704.1 ribosome silencing factor -
  VXF94_RS18480 (VXF94_18480) - 3579492..3580229 (+) 738 WP_014470703.1 class I SAM-dependent DNA methyltransferase -
  VXF94_RS18485 (VXF94_18485) comER 3580298..3581119 (-) 822 WP_014470702.1 late competence protein ComER -
  VXF94_RS18490 (VXF94_18490) comEA 3581179..3581793 (+) 615 WP_014470701.1 helix-hairpin-helix domain-containing protein Machinery gene
  VXF94_RS18495 (VXF94_18495) - 3581859..3582428 (+) 570 WP_014470700.1 ComE operon protein 2 -
  VXF94_RS18500 (VXF94_18500) - 3582429..3584781 (+) 2353 Protein_3588 DNA internalization-related competence protein ComEC/Rec2 -
  VXF94_RS18505 (VXF94_18505) - 3584800..3584934 (-) 135 WP_003152870.1 YqzM family protein -
  VXF94_RS18510 (VXF94_18510) - 3584975..3585129 (+) 155 Protein_3590 hypothetical protein -
  VXF94_RS18515 (VXF94_18515) holA 3585169..3586204 (+) 1036 Protein_3591 DNA polymerase III subunit delta -
  VXF94_RS18520 (VXF94_18520) rpsT 3586227..3586493 (-) 267 WP_003152876.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22180.40 Da        Isoelectric Point: 5.4688

>NTDB_id=932234 VXF94_RS18490 WP_014470701.1 3581179..3581793(+) (comEA) [Bacillus amyloliquefaciens strain Fad 108]
MTKVMMTERQRQYLKILGVCLAAGAVLICILLFRSEKEEPVKQQSVLGQTASAPVTDKKDTADDKIMIDIKGAVRHPGVY
EMKAGDRVTQAIKKAGGLKEKADELNVNLAEQLQDGTIVYIPSEGEEANPPKTAAGEKENASVNINTASLDELQAIPGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=932234 VXF94_RS18490 WP_014470701.1 3581179..3581793(+) (comEA) [Bacillus amyloliquefaciens strain Fad 108]
ATGACGAAAGTGATGATGACGGAGCGGCAGAGGCAGTACCTGAAAATTCTTGGCGTTTGTCTTGCGGCAGGGGCGGTTTT
GATCTGCATTTTGCTGTTTCGTTCCGAAAAAGAAGAGCCTGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCGC
CCGTCACGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATATTAAAGGAGCGGTCCGACATCCCGGCGTTTAT
GAAATGAAGGCGGGAGACAGAGTGACGCAAGCCATTAAGAAAGCCGGCGGCCTGAAGGAAAAGGCGGATGAACTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATCGTTTATATCCCGAGTGAAGGCGAAGAGGCGAATCCCCCGAAAACCG
CAGCAGGTGAAAAGGAGAATGCTTCTGTCAATATCAATACGGCATCTCTTGACGAACTTCAGGCGATTCCCGGTGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAATGGGCGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGTTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

57.436

95.588

0.549
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.945

100

0.382
  comEA/celA/cilE Streptococcus pneumoniae Rx1

35.945

100

0.382
  comEA/celA/cilE Streptococcus pneumoniae D39

35.945

100

0.382
  comEA/celA/cilE Streptococcus pneumoniae R6

35.945

100

0.382
  comEA Staphylococcus aureus MW2

35.294

100

0.382
  comEA Latilactobacillus sakei subsp. sakei 23K

33.333

100

0.382
  comEA Staphylococcus aureus N315

34.842

100

0.377
  comEA/celA/cilE Streptococcus mitis NCTC 12261

35.484

100

0.377
  comEA/celA/cilE Streptococcus mitis SK321

35.023

100

0.373