Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Q1I82_RS01715 Genome accession   NZ_CP143633
Coordinates   366904..368613 (-) Length   569 a.a.
NCBI ID   WP_330131049.1    Uniprot ID   -
Organism   Shewanella xiamenensis strain 8M34     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 361904..373613
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q1I82_RS01685 mutT 362211..362606 (-) 396 WP_037413635.1 8-oxo-dGTP diphosphatase MutT -
  Q1I82_RS01690 yacG 362693..362902 (-) 210 WP_037413634.1 DNA gyrase inhibitor YacG -
  Q1I82_RS01695 zapD 363012..363746 (-) 735 WP_037413632.1 cell division protein ZapD -
  Q1I82_RS01700 coaE 363739..364356 (-) 618 WP_099457088.1 dephospho-CoA kinase -
  Q1I82_RS01705 pilD 364438..365364 (-) 927 WP_330131047.1 A24 family peptidase Machinery gene
  Q1I82_RS01710 pilC 365513..366784 (-) 1272 WP_330131048.1 type II secretion system F family protein Machinery gene
  Q1I82_RS01715 pilB 366904..368613 (-) 1710 WP_330131049.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Q1I82_RS01720 - 368930..370369 (+) 1440 WP_330131050.1 O-antigen ligase family protein -
  Q1I82_RS01725 - 370416..370829 (-) 414 WP_413740188.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  Q1I82_RS01730 nadC 371329..372210 (-) 882 WP_330131051.1 carboxylating nicotinate-nucleotide diphosphorylase -
  Q1I82_RS01735 ampD 372434..372979 (+) 546 WP_330131052.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62804.20 Da        Isoelectric Point: 5.7404

>NTDB_id=930077 Q1I82_RS01715 WP_330131049.1 366904..368613(-) (pilB) [Shewanella xiamenensis strain 8M34]
MPSAGLHLGLSTLFIRKGLLNEEQMASAITKSRQNKQTLVTTLVQTKLVSARSIAELCYEEYGTPLLDLAEFDISAIPEE
FLNKKLIEKHRCLPLFKRGNRLYIATSDPTNIAALEEFQFSAGLHAEAILVEEDKLAKALEKVLEEDITGLDLSGMDEAA
LAGIEVTDTDKRQEENAGEASDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPISLAGRLSA
RLKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEPDQEKLYLEMLAKP
QGMILVTGPTGSGKTVSLYTGLNILNTAERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLARRLCTECKQAEQIPEHELQRLGF
TDEQIAQGFITYKPVGCEHCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIADQAKAQGMRDLRQSGLLKVLQGVTSI
AEVNRVTSF

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=930077 Q1I82_RS01715 WP_330131049.1 366904..368613(-) (pilB) [Shewanella xiamenensis strain 8M34]
ATGCCATCCGCTGGCCTTCACCTGGGATTATCCACCCTTTTCATCCGTAAAGGTCTGCTTAATGAAGAGCAGATGGCAAG
TGCCATCACCAAATCCCGCCAAAACAAACAAACCTTAGTCACTACCTTAGTGCAGACTAAGCTCGTGTCGGCCCGCAGCA
TCGCCGAGCTTTGCTATGAAGAATATGGCACGCCACTGCTGGATTTAGCCGAATTTGATATTAGTGCCATTCCGGAAGAG
TTTCTTAATAAAAAACTGATTGAAAAACACCGCTGCTTACCGCTATTTAAGCGCGGTAATCGCCTCTATATCGCCACCTC
CGACCCAACCAATATTGCCGCCCTTGAGGAATTCCAATTTAGTGCTGGCCTACATGCCGAAGCGATTTTGGTGGAAGAAG
ATAAGCTCGCTAAAGCCCTTGAAAAGGTACTAGAAGAAGATATTACTGGTCTTGATTTAAGCGGCATGGATGAAGCAGCA
CTTGCCGGTATCGAAGTTACCGATACCGATAAGCGCCAAGAAGAAAATGCTGGCGAAGCCAGTGATGACGCCCCTATTGT
TATTTATATCAACAAGATATTAACTGACGCCATTCGTAAGGGTGCTTCAGACTTACATTTTGAGCCCTATGAAAAACGCT
ACCGTATTCGTTTTCGTATTGATGGCATTTTACATGAGGTCTCAGAACCGCCCATTAGTTTAGCAGGGCGACTCTCGGCA
CGCTTAAAGGTAATGTCAAAGCTGGATATTGCTGAGCGCCGCGTCCCCCAAGATGGGCGGATTAAAATGAAGCTGTCCCG
CACTAAGTCGATTGACTTTCGGGTAAGCACCCTGCCGACCCTGTGGGGCGAAAAAATTGTAATGCGGATATTGGACTCAT
CCTCCGCACAGCTCGGCATCGAAAAACTCGGTTACGAACCCGATCAAGAAAAGCTCTACCTTGAGATGCTAGCCAAACCC
CAAGGGATGATTCTGGTGACAGGCCCAACGGGTTCGGGTAAAACCGTTTCGCTCTATACAGGCTTAAATATCCTCAATAC
CGCCGAGCGTAATATTTCCACCGCCGAAGATCCGGTGGAAATTAACCTTGAGGGGGTAAACCAAGTCCATATTAATTTAA
AAGCGGGTTTAACCTTCGCCTCAGCACTACGTTCGTTTTTGCGACAAGACCCCGATGTGGTGATGGTGGGCGAAATCCGT
GACCTTGAAACCGCCGAAATTGCCATTAAAGCGGCGCAAACTGGCCACTTAGTATTATCAACCCTGCATACTAACTCGGC
GGCAGAAACCTTAACCCGTCTGATTAATATGGGCGTACCCGGCTATAATATTGCCAGCTCAGTTAATTTAATTATTGCTC
AGCGCCTTGCACGGCGGCTTTGCACTGAATGCAAACAAGCCGAACAGATACCCGAACATGAGCTACAACGCTTAGGCTTT
ACCGATGAGCAAATCGCCCAAGGGTTTATCACCTATAAGCCTGTGGGTTGCGAGCATTGCTCTGGCGGCTATAAAGGACG
GGTAGGTATTTACGAAGTGATGAAGATGTCAGATGAAATTGCCCGTACTATTATGGAAGGGGGTAATTCATTGCAAATTG
CTGACCAAGCCAAGGCCCAAGGGATGCGCGATCTGCGGCAATCTGGGCTACTAAAAGTGCTCCAAGGTGTAACCAGTATT
GCCGAAGTGAATCGGGTAACCAGTTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.893

98.594

0.591
  pilB Acinetobacter baumannii D1279779

59.266

95.782

0.568
  pilB Legionella pneumophila strain ERS1305867

56.406

98.77

0.557
  pilB Vibrio cholerae strain A1552

53.514

97.54

0.522
  pilB Vibrio parahaemolyticus RIMD 2210633

51.313

100

0.515
  pilB Vibrio campbellii strain DS40M4

51.237

99.473

0.51
  pilF Neisseria gonorrhoeae MS11

49.47

99.473

0.492
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.606

98.067

0.388
  pilF Thermus thermophilus HB27

39.394

98.594

0.388
  pilB/pilB1 Synechocystis sp. PCC 6803

41.4

87.873

0.364