Detailed information NTG0929373

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: V1497_RS12630
• Genome accession: NZ_CP143541
• Coordinates: 2415935..2416423 (-)
• Length: 162 a.a.
• NCBI ID: WP_349407894.1
• Uniprot ID: -
• Organism: Pseudalkalibacillus sp. SCS-8
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2410935..2421423

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V1497_RS12610 (V1497_12630)	holA	2411382..2412416 (-)	1035	WP_349407890.1	DNA polymerase III subunit delta	-
V1497_RS12615 (V1497_12635)	-	2412760..2412894 (+)	135	WP_349407891.1	YqzM family protein	-
V1497_RS12620 (V1497_12640)	-	2412991..2415279 (-)	2289	WP_349407892.1	DNA internalization-related competence protein ComEC/Rec2	-
V1497_RS12625 (V1497_12645)	-	2415282..2415872 (-)	591	WP_349407893.1	ComE operon protein 2	-
V1497_RS12630 (V1497_12650)	comEA	2415935..2416423 (-)	489	WP_349407894.1	helix-hairpin-helix domain-containing protein	Machinery gene
V1497_RS12635 (V1497_12655)	comER	2416694..2417521 (+)	828	WP_349407895.1	late competence protein ComER	-
V1497_RS12640 (V1497_12660)	-	2417615..2418370 (-)	756	WP_349407896.1	class I SAM-dependent methyltransferase	-
V1497_RS12645 (V1497_12665)	rsfS	2418383..2418736 (-)	354	WP_349407897.1	ribosome silencing factor	-
V1497_RS12650 (V1497_12670)	yqeK	2418733..2419308 (-)	576	WP_349407898.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
V1497_RS12655 (V1497_12675)	-	2419301..2419876 (-)	576	WP_349407899.1	nicotinate-nucleotide adenylyltransferase	-
V1497_RS12660 (V1497_12680)	yhbY	2419883..2420176 (-)	294	WP_349407900.1	ribosome assembly RNA-binding protein YhbY	-
V1497_RS12665 (V1497_12685)	aroE	2420176..2421024 (-)	849	WP_349407901.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 162 a.a.
• Molecular weight: 17878.34 Da
• Isoelectric Point: 4.3474

>NTDB_id=929373 V1497_RS12630 WP_349407894.1 2415935..2416423(-) (comEA) [Pseudalkalibacillus sp. SCS-8]
MMNQMNVMEEEEEPPAAHQKEENVILVDLKGAVVKPGVYQMKPGQRINDLIMLAGGFQNDADADRVNLAEVLQDEAVVFV
PRKGEESPVFELQKKDSGKVDINKADQQELETIPGIGPSKAAAILNYREENGLFSKVEDLTKVAGIGEKTLERIKESIMV
SN

Nucleotide

• Length: 489 bp

>NTDB_id=929373 V1497_RS12630 WP_349407894.1 2415935..2416423(-) (comEA) [Pseudalkalibacillus sp. SCS-8]
ATGATGAACCAAATGAACGTCATGGAAGAAGAAGAGGAACCACCTGCTGCTCACCAAAAAGAGGAAAATGTCATCCTTGT
TGATTTGAAAGGTGCGGTGGTGAAACCAGGTGTATATCAGATGAAGCCCGGTCAACGGATCAATGATCTGATCATGCTCG
CAGGTGGTTTTCAGAACGATGCGGATGCTGACAGGGTGAATTTGGCAGAAGTCCTTCAAGATGAAGCGGTTGTATTTGTA
CCTAGAAAAGGTGAGGAGTCTCCTGTATTCGAACTTCAAAAAAAGGATTCTGGAAAAGTAGATATCAACAAAGCTGATCA
ACAGGAGCTGGAGACGATTCCTGGAATCGGTCCATCGAAAGCGGCAGCTATATTGAATTACAGGGAGGAAAATGGCCTGT
TTTCCAAGGTGGAAGATTTGACGAAGGTCGCAGGGATCGGAGAAAAGACGTTGGAGAGAATCAAGGAATCGATAATGGTG
TCCAATTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (27-76)

• Helix-hairpin-helix motif (HHH_3) (97-158)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	51.974	93.827	0.488
comEA	Lactococcus lactis subsp. cremoris KW2	44.156	95.062	0.42
comEA	Streptococcus thermophilus LMD-9	43.312	96.914	0.42
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	42.953	91.975	0.395
comEA/celA/cilE	Streptococcus mitis SK321	42.568	91.358	0.389
comEA/celA/cilE	Streptococcus mitis NCTC 12261	42.466	90.123	0.383
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.892	91.358	0.383
comEA/celA/cilE	Streptococcus pneumoniae D39	41.892	91.358	0.383
comEA/celA/cilE	Streptococcus pneumoniae R6	41.892	91.358	0.383
comEA	Latilactobacillus sakei subsp. sakei 23K	37.423	100	0.377