Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   V0242_RS02410 Genome accession   NZ_CP143514
Coordinates   506348..508054 (-) Length   568 a.a.
NCBI ID   WP_029302067.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain MAH-4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 501348..513054
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V0242_RS02380 (V0242_02380) mutT 501984..502397 (+) 414 WP_330543153.1 8-oxo-dGTP diphosphatase MutT -
  V0242_RS02385 (V0242_02385) yacG 502458..502652 (-) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  V0242_RS02390 (V0242_02390) zapD 502662..503384 (-) 723 WP_330543154.1 cell division protein ZapD -
  V0242_RS02395 (V0242_02395) coaE 503422..504036 (-) 615 WP_330543156.1 dephospho-CoA kinase -
  V0242_RS02400 (V0242_02400) pilD 504056..504928 (-) 873 WP_330543157.1 A24 family peptidase Machinery gene
  V0242_RS02405 (V0242_02405) pilC 504991..506232 (-) 1242 WP_330543160.1 type II secretion system F family protein Machinery gene
  V0242_RS02410 (V0242_02410) pilB 506348..508054 (-) 1707 WP_029302067.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  V0242_RS02415 (V0242_02415) - 508058..508495 (-) 438 WP_254201361.1 pilin -
  V0242_RS02420 (V0242_02420) nadC 508818..509675 (-) 858 WP_330543164.1 carboxylating nicotinate-nucleotide diphosphorylase -
  V0242_RS02425 (V0242_02425) - 509681..510151 (-) 471 WP_024944772.1 TIGR02281 family clan AA aspartic protease -
  V0242_RS02430 (V0242_02430) ampD 510298..510870 (+) 573 WP_330543168.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  V0242_RS02435 (V0242_02435) pdhR 511146..511910 (+) 765 WP_011707564.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62511.90 Da        Isoelectric Point: 5.4342

>NTDB_id=929260 V0242_RS02410 WP_029302067.1 506348..508054(-) (pilB) [Aeromonas hydrophila strain MAH-4]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTTAIGKLMESDQDALGMEDIDESEI
SELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=929260 V0242_RS02410 WP_029302067.1 506348..508054(-) (pilB) [Aeromonas hydrophila strain MAH-4]
ATGACTTCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCGCTGCTCAGTGAGTCTGACTCACAACGCTA
TCTGAGCCAAGCCAAGGCACAGCGCAAACCTTTTGTGACCTTTCTGATCGAAAACGAAATCCTCGACAGCAAGGCACTGG
CTGACTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATACCGCAGAAATAC
CTCAATCAGAAGCTGATTGAAAAACATCACGTCCTGCCCATCTACACCCAAGGACACACCCTCTATATCGCCATGTCAGA
CCCGACCAATGTCTCGGCACTGGAGGATTTTGGCTTTAGCTTCGGGTTGCATACCGAGGCACTGCTGGTCGAGGAGAACA
AACTCACCACAGCCATCGGCAAGCTGATGGAGAGCGATCAGGATGCCCTCGGCATGGAGGACATCGACGAATCCGAGATC
TCCGAACTGGAGGTCTCGGACGAGGGATCGCGGCTCGACGAAAGTGTCAATACGGCCGACGATGACGCGCCGATCGTCAA
ATACATCAACAAGATCATGATGGATGCCATCAAACGCGGGGCCTCCGACCTGCACTTCGAACCCTACGAGACCAAATACC
GCATCCGCTTTCGGATCGACGGCATATTGCACGAAATCGCGACACCACCGGTCAATCTGGCCAACCGCTTCTCGGCCCGC
CTCAAGGTCATGGCGCGGCTCGACATCGCCGAACGGCGGCTGCCGCAGGATGGCCGCATCAAGCTCAAGCTGTCGCGCAA
CAAGTCGATGGACATGCGGGTCAATACCCTGCCCACCATGTGGGGCGAAAAAATCGTTATCCGTCTGCTCGATTCCTCCG
CCGCACGCCTCAATATCGAGCAGCTCGGTTTTGACGATCGGCAGAAGGCGCAATACCTACGTGCCCTCTCGAAGCCTCAA
GGCATGATCCTGGTGACCGGCCCTACCGGCTCGGGCAAGACGGTTTCACTTTATACCGGCCTCAATATTCTCAATACCAC
CGAGGTCAATATCTCTACCGCCGAAGATCCGGTGGAGATCAATCTGCCCGGCGTCAATCAGGTGCAAGTCAATCCCAAGG
CGGGGCTGACCTTCGCCAGCGCCCTGCGCTCTTTCTTGCGGCAAGACCCCGATGTGGTCATGGTGGGTGAAATACGGGAT
CTGGAAACAGCCGAAATTGCCATCAAGGCTGCCCAGACCGGCCACCTGGTGCTCTCCACCCTGCATACCAACTCGGCGGC
GGAAACGCTGACCCGGATGATGAACATGGGGGTACCCGCCTTCAATATCGCCTCCTCGGTCACCCTGATCATGGCTCAGC
GGCTGGCACGCAAACTGTGCGATCACTGCAAAGCCCCCGAAGTGGTGCCCGAGGCTGAGCTGCTTGAGCTGGGTTTTACC
CAGCAGCAGCTGGCCGCTGGCTTGCGGCTGTTCAAACCGGTTGGCTGCAAAGAGTGCTCGGGTGGCTACAAGGGCCGGGT
CGGCATCTACGAAATCATGCTAATGTCTGAAAACATCGCAAAATTGATCATGCAGGGGGCCAACTCTCTGCAGATTGCCG
CCATCGCCCAGAAGGAAGGCATGCGTACCTTGCGTACCTCCGGCCTTGAGAAGGCCCGCCTAGGGGTGACCAGTCTGGCG
GAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.712

100

0.558
  pilB Acinetobacter baylyi ADP1

55.439

100

0.556
  pilB Legionella pneumophila strain ERS1305867

54.336

99.472

0.54
  pilB Vibrio cholerae strain A1552

54.159

99.472

0.539
  pilB Vibrio campbellii strain DS40M4

51.943

99.648

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.423

98.944

0.509
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.152

92.958

0.373