Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   VXM67_RS03750 Genome accession   NZ_CP142890
Coordinates   819662..820930 (+) Length   422 a.a.
NCBI ID   WP_084212786.1    Uniprot ID   -
Organism   Pseudomonas sp. Rh2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 814662..825930
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VXM67_RS03725 (VXM67_03725) - 814686..817139 (-) 2454 WP_027907909.1 penicillin-binding protein 1A -
  VXM67_RS03730 (VXM67_03730) - 817311..817538 (+) 228 WP_367237503.1 hypothetical protein -
  VXM67_RS03735 (VXM67_03735) pilM 817545..818201 (+) 657 WP_367237504.1 type IV pilus biogenesis protein PilM -
  VXM67_RS03740 (VXM67_03740) - 818189..818725 (+) 537 WP_023378224.1 PilN domain-containing protein -
  VXM67_RS03745 (VXM67_03745) - 818782..819729 (+) 948 WP_238546532.1 pilus assembly protein PilP -
  VXM67_RS03750 (VXM67_03750) pilQ 819662..820930 (+) 1269 WP_084212786.1 type IV pilus secretin PilQ Machinery gene
  VXM67_RS03755 (VXM67_03755) aroK 820935..821453 (+) 519 WP_023378227.1 shikimate kinase AroK -
  VXM67_RS03760 (VXM67_03760) aroB 821551..822648 (+) 1098 WP_023378228.1 3-dehydroquinate synthase -
  VXM67_RS03765 (VXM67_03765) - 822664..824289 (+) 1626 WP_104883044.1 AAA family ATPase -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 45817.66 Da        Isoelectric Point: 5.0190

>NTDB_id=927479 VXM67_RS03750 WP_084212786.1 819662..820930(+) (pilQ) [Pseudomonas sp. Rh2]
MKCLLRWIAAALLVVPPVMAAPDRGESLSLNFQDVPVRSVLQVMADYAGINLVASDAVQGNITLRLQDVPWDQALELVLR
SKGLGRRQEGNVLLVAPAAELAEQSRGERIGQELEAQLQPLSRALLPIHHAKASELVELLQATLADDGILTGRGSLAVDA
RTNTLIAHQPADRLAELRRLVAELDVPLRQVAIEARIVEANVDYEKSLGVRWGGPLYGETAQLGRDLFVDLGVDRATSGV
GLGLLRAEVLLDLELSAMEKSGNGEIISQPKVVTGDKETARILKGTEVPYQETSKSGATSVAFREASLSLEVTPQITPDG
KVIMAVRVTKDEPDYVNALNDVPPIRKNEVNARVRVADGETIVIGGVYSTTQNKVIDKVPFFGDLPYVGRLFRRDALQEK
KSELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=927479 VXM67_RS03750 WP_084212786.1 819662..820930(+) (pilQ) [Pseudomonas sp. Rh2]
ATGAAATGCCTGTTGAGGTGGATCGCGGCGGCGTTGTTGGTAGTCCCGCCGGTCATGGCGGCGCCTGACCGGGGGGAGTC
GCTGTCGCTGAATTTCCAGGATGTGCCCGTGCGCTCAGTGTTGCAGGTGATGGCCGACTACGCGGGCATCAACCTGGTAG
CCAGCGATGCCGTGCAAGGCAACATCACGTTGAGATTGCAGGATGTGCCGTGGGATCAGGCGCTGGAGCTGGTGTTGCGC
AGCAAGGGGCTGGGCCGTCGCCAAGAGGGCAATGTGTTGCTTGTGGCGCCGGCCGCCGAGTTGGCCGAGCAGTCCCGTGG
CGAGCGGATCGGGCAGGAGCTGGAGGCTCAGTTGCAGCCCCTGAGCCGGGCATTGCTGCCCATCCACCATGCCAAGGCTT
CTGAACTTGTCGAGTTGCTCCAGGCGACCCTGGCGGACGACGGTATTCTGACCGGTCGCGGCAGCCTGGCGGTCGATGCG
CGTACCAACACGCTGATCGCCCACCAGCCAGCCGATAGGCTGGCCGAATTGCGCAGATTGGTGGCCGAACTGGACGTGCC
GCTGCGGCAAGTGGCGATCGAGGCGCGCATCGTCGAGGCCAATGTCGACTACGAAAAGAGCCTGGGCGTGCGCTGGGGTG
GACCGCTGTATGGCGAAACGGCGCAGTTGGGTCGTGATTTGTTCGTTGACTTGGGGGTAGACAGGGCGACCTCCGGGGTG
GGGTTGGGCTTGCTGCGCGCGGAAGTGCTGCTGGATCTTGAGCTCAGTGCCATGGAGAAAAGCGGCAACGGCGAAATCAT
CTCGCAGCCCAAGGTGGTGACGGGCGACAAGGAAACCGCCAGAATTCTCAAAGGCACCGAAGTGCCATATCAGGAGACCA
GCAAGAGCGGCGCCACGTCTGTCGCTTTCCGCGAGGCATCGTTGTCCCTCGAGGTCACGCCGCAGATCACCCCAGACGGC
AAGGTGATCATGGCAGTCAGAGTGACCAAGGACGAGCCGGACTACGTCAACGCCTTGAACGACGTGCCTCCGATCCGCAA
GAACGAGGTCAATGCCAGGGTTCGTGTAGCTGATGGCGAAACGATAGTGATCGGCGGAGTCTATTCGACCACGCAAAACA
AAGTGATCGACAAGGTGCCATTTTTTGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAA
AAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(125-190)

Bacterial type II and III secretion system protein (Secretin)

(257-413)

Secretin and TonB N terminus short domain (STN)

(51-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

59.677

100

0.614
  pilQ Acinetobacter baumannii D1279779

40.351

100

0.436
  pilQ Acinetobacter baumannii strain A118

40.351

100

0.436
  comQ Acinetobacter baylyi ADP1

41.163

100

0.436
  pilQ Legionella pneumophila strain Lp02

42.788

98.578

0.422
  pilQ Legionella pneumophila strain ERS1305867

42.788

98.578

0.422
  pilQ Vibrio campbellii strain DS40M4

40.185

100

0.412
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.205

98.341

0.405
  pilQ Vibrio cholerae strain A1552

41.205

98.341

0.405