Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   VJ143_RS01585 Genome accession   NZ_CP142740
Coordinates   338233..339948 (-) Length   571 a.a.
NCBI ID   WP_327858021.1    Uniprot ID   -
Organism   Acinetobacter guillouiae strain 13-3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 333233..344948
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VJ143_RS01560 (VJ143_01560) rlmB 333689..334444 (+) 756 WP_105714634.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  VJ143_RS01565 (VJ143_01565) - 334483..335394 (+) 912 WP_327858020.1 DMT family transporter -
  VJ143_RS01570 (VJ143_01570) coaE 335463..336062 (-) 600 WP_096732748.1 dephospho-CoA kinase -
  VJ143_RS01575 (VJ143_01575) pilD 336089..336970 (-) 882 WP_262782506.1 prepilin peptidase Machinery gene
  VJ143_RS01580 (VJ143_01580) pilC 336976..338205 (-) 1230 WP_262782508.1 type II secretion system F family protein Machinery gene
  VJ143_RS01585 (VJ143_01585) pilB 338233..339948 (-) 1716 WP_327858021.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VJ143_RS01590 (VJ143_01590) tpiA 340226..341020 (+) 795 WP_327858022.1 triose-phosphate isomerase -
  VJ143_RS01595 (VJ143_01595) secG 341030..341371 (+) 342 WP_004718341.1 preprotein translocase subunit SecG -
  VJ143_RS01620 (VJ143_01620) rimP 342162..342686 (+) 525 WP_004718339.1 ribosome maturation factor RimP -
  VJ143_RS01625 (VJ143_01625) nusA 342721..344205 (+) 1485 WP_004718337.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63622.09 Da        Isoelectric Point: 4.9189

>NTDB_id=926635 VJ143_RS01585 WP_327858021.1 338233..339948(-) (pilB) [Acinetobacter guillouiae strain 13-3]
MSMQNGSPKFSGYIRRLVDEGFLSAQQMKDALNNAQKNKVSIVAYLSEKLNIPPLIIAEMISQEFGEPIFDLDSFNPEML
PKELIDSNLVTKHKVIPLVQRGNILYVATSDPTNIEALDAIRFNCGLNIEPIIVEHHKLEKYLEKHFTVEESFNFDGEDF
DVDIIATDEQPPSEEDDDEASEEEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRTITNPPLQLASRLA
SRLKVMSRMDISEKRVPQDGRIKLKISKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEKQKALFMEALEK
PQGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNLKAGLSFSVALKSFLRQDPDIIMVGEI
RDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCNHCKAPVEIPRQSLLEMG
FTEQDLSNPDLQIFQPVGCSECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAAASEKLGFDNLRRSGLIKVINGLT
SLQEINRVTSE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=926635 VJ143_RS01585 WP_327858021.1 338233..339948(-) (pilB) [Acinetobacter guillouiae strain 13-3]
ATGTCTATGCAAAATGGTTCACCTAAGTTTTCGGGGTATATCCGTCGCTTAGTCGACGAAGGTTTTTTATCTGCCCAGCA
AATGAAAGATGCGCTCAATAATGCTCAAAAAAATAAAGTGAGTATCGTGGCTTATCTTAGTGAAAAATTAAATATACCGC
CACTGATCATTGCTGAAATGATCTCCCAAGAATTTGGAGAACCCATTTTTGATTTGGACAGCTTTAATCCTGAGATGCTG
CCCAAGGAGCTTATTGACTCAAATTTAGTCACTAAACATAAAGTGATTCCTTTAGTACAACGTGGCAATATTTTATATGT
CGCCACCAGTGATCCGACCAATATAGAAGCGCTGGATGCCATTCGTTTTAACTGCGGTTTGAATATTGAACCAATCATTG
TTGAACATCATAAATTAGAAAAATATTTAGAAAAGCACTTTACGGTAGAGGAAAGCTTTAATTTTGATGGTGAAGACTTT
GATGTTGATATCATCGCAACCGATGAACAACCGCCATCAGAAGAAGATGACGATGAGGCTTCAGAAGAAGAAGCGCCTAT
TGTTAAATATATCAATAAGCTTTTGATCGATGCGATTCGTATGGGTGCATCAGATTTACACTTTGAACCTTATGAAAAAA
GTTATCGGGTCCGTTATCGTGTAGATGGTGTACTGAGAACCATTACTAATCCCCCTTTGCAATTAGCTAGCCGCTTGGCA
TCACGCTTAAAAGTGATGTCTAGAATGGATATTTCTGAAAAGCGTGTTCCACAGGATGGTCGTATTAAGTTAAAAATATC
AAAGACCAAAGCTATTGATTTTCGTGTGAATTCATTACCTACATTGTTTGGCGAAAAATTGGTACTGCGTATTCTTGATC
CTTCCAGTGCCATGTTGGGAATTGATGCACTAGGTTATGAAGAAAAGCAAAAAGCATTATTTATGGAGGCCTTAGAAAAA
CCTCAAGGCATGTTACTGATTACAGGTCCCACAGGTTCAGGTAAAACTGTTTCCCTCTATACAGGTTTAAATATCCTTAA
TACAGAAAGTTCAAATATCTCCACCGCTGAAGATCCAGTAGAAATTAACTTAGAAGGGATCAATCAGGTCAATGTTAATC
TTAAAGCAGGCCTCAGCTTTTCAGTCGCGCTCAAATCATTCCTGCGTCAAGATCCTGACATTATCATGGTCGGGGAGATT
CGAGACCTCGAAACTGCTGAAATTGCGATCAAGGCAGCTCAAACGGGTCATATGGTGATGTCTACCCTGCATACCAACAG
TGCACCTGAAACATTGACCCGTTTACGTAATATGGGTGTACCTTCATTTAACATTGCCACCTCGGTCAATCTTGTGATTG
CACAACGATTGGCAAGACGTTTATGTAATCATTGTAAAGCACCCGTTGAAATTCCTAGGCAGAGTCTGTTAGAAATGGGC
TTTACTGAACAAGACTTAAGCAATCCTGACTTACAAATTTTTCAACCTGTGGGCTGTAGTGAATGCCGTGAAGGCTATAA
AGGTCGTGTCGGGATCTACGAAGTGATGAAAGTAACACCTGAAATTTCAAAAATTATCATGGAAGATGGCAATGCACTTC
AAATTGCAGCGGCATCTGAAAAATTGGGGTTTGATAATCTAAGGCGTTCAGGTTTAATTAAGGTCATCAATGGCTTAACT
TCTTTACAAGAAATTAACCGTGTGACCAGCGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

78.846

100

0.79
  pilB Acinetobacter baylyi ADP1

75.306

100

0.753
  pilB Legionella pneumophila strain ERS1305867

51.038

100

0.517
  pilF Neisseria gonorrhoeae MS11

49.468

98.774

0.489
  pilB Vibrio cholerae strain A1552

50.674

90.893

0.461
  pilB Vibrio parahaemolyticus RIMD 2210633

50.579

90.718

0.459
  pilB Vibrio campbellii strain DS40M4

49.71

90.543

0.45
  pilB/pilB1 Synechocystis sp. PCC 6803

34.91

100

0.375
  pilF Thermus thermophilus HB27

36.077

100

0.361