Detailed information NTG0092516

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: Llc71_RS09720
• Genome accession: NZ_AP025520
• Coordinates: 1934940..1935554 (-)
• Length: 204 a.a.
• NCBI ID: WP_075070464.1
• Uniprot ID: -
• Organism: Lactococcus cremoris strain 7-1
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1929940..1940554

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Llc71_RS09695 (Llc71_19450)	atpF	1930010..1930516 (-)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-
Llc71_RS09700 (Llc71_19460)	atpB	1930531..1931244 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
Llc71_RS09705 (Llc71_19470)	-	1931291..1931506 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
Llc71_RS09710 (Llc71_19480)	-	1931689..1932465 (-)	777	WP_011835694.1	alpha/beta hydrolase family protein	-
Llc71_RS09715 (Llc71_19490)	comEC	1932749..1934959 (-)	2211	WP_046124455.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
Llc71_RS09720 (Llc71_19500)	comEA	1934940..1935554 (-)	615	WP_075070464.1	helix-hairpin-helix domain-containing protein	Machinery gene
Llc71_RS09725 (Llc71_19510)	-	1935654..1937015 (-)	1362	WP_011835696.1	ABC transporter permease	-
Llc71_RS09730 (Llc71_19520)	-	1937012..1937944 (-)	933	WP_046124456.1	ABC transporter ATP-binding protein	-
Llc71_RS09735	-	1938050..1938448 (-)	399	WP_042747837.1	hypothetical protein	-
Llc71_RS09740 (Llc71_19530)	-	1938565..1939125 (-)	561	WP_011835699.1	GNAT family N-acetyltransferase	-
Llc71_RS09745 (Llc71_19540)	-	1939299..1940477 (-)	1179	WP_021037692.1	SLC13 family permease	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 21872.61 Da
• Isoelectric Point: 4.9295

>NTDB_id=92516 Llc71_RS09720 WP_075070464.1 1934940..1935554(-) (comEA) [Lactococcus cremoris strain 7-1]
MLLVVCALIAGGILYIFTNSPKPAETLSVENLSSSSTKSSVSKFNSSSSEKNKNEIMVDLKGAVAKPNVYQISSDERLVD
LIRQAGGFTDQADQKSINLSAKLKDEEVIYVPKVGESSSSESTDSPTGSSVSNQVSTTSGPKVNINKADLTELQKLTGIG
QKKAQDIIDFRMKNGDFKSIEDLGKVSGFGDKTLEKLKDEISID

Nucleotide

• Length: 615 bp

>NTDB_id=92516 Llc71_RS09720 WP_075070464.1 1934940..1935554(-) (comEA) [Lactococcus cremoris strain 7-1]
ATGCTTTTAGTTGTTTGTGCACTTATTGCTGGTGGAATTCTTTATATTTTCACCAATTCACCAAAGCCAGCTGAAACTCT
GTCAGTAGAAAATTTGAGCTCTAGTTCTACCAAAAGCTCTGTCAGTAAATTTAATAGTAGCAGTAGTGAAAAAAATAAAA
ATGAAATTATGGTTGATTTAAAAGGAGCGGTTGCAAAACCTAATGTATACCAAATTTCGTCAGATGAGCGCCTTGTTGAT
TTAATTAGGCAAGCGGGAGGATTTACTGACCAAGCAGACCAAAAATCAATCAATCTGTCAGCGAAACTTAAAGATGAAGA
AGTGATTTATGTACCAAAAGTTGGGGAAAGTTCAAGTTCAGAAAGTACTGACAGTCCTACTGGTAGCTCTGTCAGTAATC
AAGTTTCAACGACAAGTGGTCCAAAAGTAAATATCAATAAAGCAGACCTGACAGAATTACAAAAACTGACTGGAATTGGT
CAAAAAAAAGCGCAAGATATTATTGATTTTCGCATGAAAAATGGTGACTTTAAATCAATAGAGGATTTGGGTAAAGTATC
TGGCTTTGGGGATAAAACATTAGAAAAACTGAAAGATGAGATTTCTATTGATTAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (57-112)

• Helix-hairpin-helix motif (HHH_3) (140-201)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	99.02	100	0.99
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	41.395	100	0.436
comEA/celA/cilE	Streptococcus mitis SK321	41.315	100	0.431
comEA	Latilactobacillus sakei subsp. sakei 23K	39.269	100	0.422
comEA/celA/cilE	Streptococcus mitis NCTC 12261	39.535	100	0.417
comEA/celA/cilE	Streptococcus pneumoniae D39	40.476	100	0.417
comEA/celA/cilE	Streptococcus pneumoniae Rx1	40.476	100	0.417
comEA/celA/cilE	Streptococcus pneumoniae R6	40.476	100	0.417
comEA	Bacillus subtilis subsp. subtilis str. 168	37.438	99.51	0.373