Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   VFH86_RS02320 Genome accession   NZ_CP142014
Coordinates   485356..485829 (-) Length   157 a.a.
NCBI ID   WP_331305089.1    Uniprot ID   -
Organism   Vibrio cholerae strain LK-18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 480356..490829
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VFH86_RS02300 (VFH86_02300) coaE 480852..481460 (-) 609 WP_033928827.1 dephospho-CoA kinase -
  VFH86_RS02305 (VFH86_02305) pilD 481457..482332 (-) 876 WP_114762862.1 A24 family peptidase Machinery gene
  VFH86_RS02310 (VFH86_02310) pilC 482390..483616 (-) 1227 WP_000648510.1 type II secretion system F family protein Machinery gene
  VFH86_RS02315 (VFH86_02315) pilB 483659..485347 (-) 1689 WP_148524149.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VFH86_RS02320 (VFH86_02320) pilA 485356..485829 (-) 474 WP_331305089.1 pilin Machinery gene
  VFH86_RS02325 (VFH86_02325) nadC 486074..486964 (-) 891 WP_000665275.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VFH86_RS02330 (VFH86_02330) ampD 487266..487811 (+) 546 WP_001959072.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VFH86_RS02335 (VFH86_02335) fldB 487896..488414 (-) 519 WP_000690112.1 flavodoxin FldB -
  VFH86_RS02340 (VFH86_02340) xerD 488552..489460 (+) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  VFH86_RS02345 (VFH86_02345) dsbC 489477..490229 (+) 753 WP_345658289.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 157 a.a.        Molecular weight: 16277.74 Da        Isoelectric Point: 7.7737

>NTDB_id=920929 VFH86_RS02320 WP_331305089.1 485356..485829(-) (pilA) [Vibrio cholerae strain LK-18]
MKAYKNKQQQGFTLIELMIVVAVIGVLAAIAIPQYQNYVKKSEAAAAVATVRSLTTNIDTYIADAGTFPSDTNFADIGAA
AGMNKLGTIALDAATKTVKLTFLANNSVYENQETVVMTKGSDGLWACTVPTGVTLKGCTAAAATPPTPPTPPTPPTP

Nucleotide


Download         Length: 474 bp        

>NTDB_id=920929 VFH86_RS02320 WP_331305089.1 485356..485829(-) (pilA) [Vibrio cholerae strain LK-18]
ATGAAAGCGTATAAAAACAAACAACAGCAAGGTTTTACCTTAATTGAATTGATGATTGTGGTGGCAGTGATTGGTGTGTT
GGCAGCGATTGCAATTCCTCAATATCAAAACTACGTAAAAAAATCAGAGGCGGCAGCGGCAGTAGCGACAGTGCGCTCTT
TGACTACGAATATAGACACTTACATTGCAGATGCAGGAACTTTCCCATCTGATACCAACTTCGCGGATATTGGTGCAGCA
GCAGGTATGAATAAATTAGGTACTATAGCTCTAGACGCTGCTACTAAGACTGTGAAACTAACATTCTTAGCTAACAATTC
TGTTTATGAGAACCAAGAGACCGTAGTCATGACTAAAGGTTCTGATGGTTTATGGGCTTGTACAGTACCAACAGGTGTAA
CCCTAAAAGGATGTACAGCTGCAGCAGCCACACCACCAACACCACCAACACCACCAACACCACCAACACCATAG

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(41-134)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

50.318

100

0.503
  pilA Vibrio cholerae strain A1552

50.318

100

0.503
  pilA Vibrio cholerae C6706

50.318

100

0.503
  pilA Pseudomonas aeruginosa PAK

44.595

94.268

0.42
  pilA Vibrio parahaemolyticus RIMD 2210633

42.657

91.083

0.389
  pilA Acinetobacter baumannii strain A118

40.972

91.72

0.376