NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	VA609_RS04365	Genome accession	NZ_CP142012
Coordinates	982845..983279 (-)	Length	144 a.a.
NCBI ID	WP_005741096.1	Uniprot ID	-
Organism	Pasteurella multocida strain PM-2
Function	type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 977845..988279

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VA609_RS04335 (VA609_04335)	-	978409..978678 (-)	270	WP_324739503.1	GNAT family N-acetyltransferase	-
VA609_RS04340 (VA609_04340)	yacG	978678..978881 (-)	204	WP_005719879.1	DNA gyrase inhibitor YacG	-
VA609_RS04345 (VA609_04345)	coaE	978892..979512 (-)	621	WP_005753357.1	dephospho-CoA kinase	-
VA609_RS04350 (VA609_04350)	-	979527..980249 (-)	723	WP_064968698.1	prepilin peptidase	-
VA609_RS04355 (VA609_04355)	pilC	980249..981460 (-)	1212	WP_324739548.1	type II secretion system F family protein	Machinery gene
VA609_RS04360 (VA609_04360)	pilB	981460..982845 (-)	1386	WP_046333442.1	GspE/PulE family protein	Machinery gene
VA609_RS04365 (VA609_04365)	pilA	982845..983279 (-)	435	WP_005741096.1	prepilin peptidase-dependent pilin	Machinery gene
VA609_RS04370 (VA609_04370)	ampD	983439..984005 (+)	567	WP_324739504.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
VA609_RS04375 (VA609_04375)	rppH	984592..985059 (+)	468	Protein_841	RNA pyrophosphohydrolase	-
VA609_RS04380 (VA609_04380)	-	985191..985985 (+)	795	WP_005722415.1	sulfite exporter TauE/SafE family protein	-
VA609_RS04385 (VA609_04385)	lgt	985995..986798 (+)	804	WP_005751073.1	prolipoprotein diacylglyceryl transferase	-
VA609_RS04390 (VA609_04390)	-	986804..987655 (+)	852	WP_324739505.1	thymidylate synthase	-
VA609_RS04395 (VA609_04395)	tadA	987670..988170 (+)	501	WP_005755875.1	tRNA adenosine(34) deaminase TadA	-

Sequence

Protein

Download Length: 144 a.a. Molecular weight: 15058.48 Da Isoelectric Point: 9.1281

                                >NTDB_id=920897 VA609_RS04365 WP_005741096.1 982845..983279(-) (pilA) [Pasteurella multocida strain PM-2]

MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR

ADAAASKKYLKAITVKAGVITVTGQGSLEGISYTLTASGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide

Download Length: 435 bp

                                >NTDB_id=920897 VA609_RS04365 WP_005741096.1 982845..983279(-) (pilA) [Pasteurella multocida strain PM-2]

ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT

TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCAGCTC

CTTATCGTGCTGAGGTAGAGCTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT

GCTGATGCTGCAGCAAGTAAAAAATATTTAAAAGCCATCACGGTTAAAGCTGGGGTAATTACTGTCACTGGACAAGGGAG

CTTGGAAGGAATTAGCTATACGCTGACAGCGAGTGGTAACGCAGCTCAAGGTGTTTCTTGGACAGTTAATTGTGGTGCTC

AATCTGACATTTTCCCAGCAGGATTTTGTGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Haemophilus influenzae 86-028NP	63.194	100	0.632
pilA	Haemophilus influenzae Rd KW20	61.111	100	0.611
pilA	Glaesserella parasuis strain SC1401	52.349	100	0.542
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	36.806	100	0.368
pilA	Vibrio cholerae strain A1552	36.806	100	0.368
pilA	Vibrio cholerae C6706	36.806	100	0.368

Detailed information NTG0920897