Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   VA609_RS04360 Genome accession   NZ_CP142012
Coordinates   981460..982845 (-) Length   461 a.a.
NCBI ID   WP_046333442.1    Uniprot ID   -
Organism   Pasteurella multocida strain PM-2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 976460..987845
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VA609_RS04330 (VA609_04330) - 976587..977960 (-) 1374 WP_005722571.1 alanine/glycine:cation symporter family protein -
  VA609_RS04335 (VA609_04335) - 978409..978678 (-) 270 WP_324739503.1 GNAT family N-acetyltransferase -
  VA609_RS04340 (VA609_04340) yacG 978678..978881 (-) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  VA609_RS04345 (VA609_04345) coaE 978892..979512 (-) 621 WP_005753357.1 dephospho-CoA kinase -
  VA609_RS04350 (VA609_04350) - 979527..980249 (-) 723 WP_064968698.1 prepilin peptidase -
  VA609_RS04355 (VA609_04355) pilC 980249..981460 (-) 1212 WP_324739548.1 type II secretion system F family protein Machinery gene
  VA609_RS04360 (VA609_04360) pilB 981460..982845 (-) 1386 WP_046333442.1 GspE/PulE family protein Machinery gene
  VA609_RS04365 (VA609_04365) pilA 982845..983279 (-) 435 WP_005741096.1 prepilin peptidase-dependent pilin Machinery gene
  VA609_RS04370 (VA609_04370) ampD 983439..984005 (+) 567 WP_324739504.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VA609_RS04375 (VA609_04375) rppH 984592..985059 (+) 468 Protein_841 RNA pyrophosphohydrolase -
  VA609_RS04380 (VA609_04380) - 985191..985985 (+) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  VA609_RS04385 (VA609_04385) lgt 985995..986798 (+) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  VA609_RS04390 (VA609_04390) - 986804..987655 (+) 852 WP_324739505.1 thymidylate synthase -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52033.67 Da        Isoelectric Point: 6.2343

>NTDB_id=920896 VA609_RS04360 WP_046333442.1 981460..982845(-) (pilB) [Pasteurella multocida strain PM-2]
MFHENPLCTVSAMTGEVFEIQQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIEPVLLS
TNELKRALQQLSPPKLQVEENIPTVYQVAQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPPFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFAELGM
TADQQHRFQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSIEKHILTAEDPIEIALTGIIQTQVNTQIGLDFKRLLRT
FLRQDPDIIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRRCDCSV
ETQSACCCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=920896 VA609_RS04360 WP_046333442.1 981460..982845(-) (pilB) [Pasteurella multocida strain PM-2]
ATGTTTCATGAGAATCCTTTGTGTACTGTTAGTGCAATGACAGGAGAGGTATTTGAAATCCAACAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGTTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAGCTGTGGCTTGGCG
TGGATTCGTTAGCGAATTTATCAGCATGTGATGCCTTTTCTTTTTTAACGAATAAACACATTGAGCCTGTTTTATTATCA
ACAAATGAACTGAAACGGGCATTGCAGCAACTTTCCCCACCAAAATTACAAGTGGAAGAAAACATACCCACGGTTTATCA
AGTCGCGCAATTAGATTCTGATTCCGCGACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGACATTCATTTTGAACCGCAACAGCATAGCTTACAAGTTCGTTTTAGAATCGATGGTGTATTACAA
GTCCAGCCTCCCTTTTCGGGAATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGA
AACACGCTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACATTTTCTGATACATTAGATTTTCGACTTTCTACGTTGC
CGACGCATTTAGGCGAAAAAGCGGTATTACGTTTGCAGCGAAATAAGCCTATTCATCTCAGTTTTGCTGAGTTGGGGATG
ACCGCAGACCAACAACACAGGTTCCAACACGCGTTAAGTCAGCCACAAGGACTGATTTTAGTAACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTATAGTGCGCTAGCTTGGCTCAACAGCATAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGCGTTGACTGGGATAATCCAAACGCAAGTAAATACGCAAATAGGACTGGATTTCAAGCGCTTATTACGCACT
TTTTTACGTCAAGATCCAGACATTATTATGTTAGGGGAAATTCGTGATGAAGAAAGCGCGTTAATCGCATTACGTGCGGC
TCAAACTGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCTCCCTCTGCTCTTACTCGCTTACAGCAATTAGGGG
TGCCTTTGCATGAAATTGAACAAAGCCTATTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTCGTTGTGATTGCAGCGTA
GAGACACAATCGGCGTGTTGTTGCTATCAGGGGTATAAAGGGAGGATTGGGGTATATCAATTTTTACAAAAAGATAAAAC
AGGGTGTTTTGTCACGGATTTTAGTAGTTTACGCGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACCGATGACAATG
AAGTACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-93)

Type II/IV secretion system protein (T2SSE)

(128-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

68.212

98.265

0.67
  pilB Haemophilus influenzae Rd KW20

67.55

98.265

0.664
  pilB Glaesserella parasuis strain SC1401

58.15

98.482

0.573
  pilB Acinetobacter baumannii D1279779

40.202

100

0.432
  pilB Vibrio parahaemolyticus RIMD 2210633

39.959

100

0.419
  pilB Vibrio cholerae strain A1552

39.22

100

0.414
  pilB Acinetobacter baylyi ADP1

38.124

100

0.414
  pilB Vibrio campbellii strain DS40M4

39.583

100

0.412
  pilB Legionella pneumophila strain ERS1305867

37.678

100

0.401