Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OCU78_RS11765 Genome accession   NZ_AP025490
Coordinates   2730080..2731765 (+) Length   561 a.a.
NCBI ID   WP_137373381.1    Uniprot ID   -
Organism   Vibrio gallaecicus strain CECT 7244     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2725080..2736765
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU78_RS11745 pdhR 2726572..2727333 (-) 762 WP_137373617.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  OCU78_RS11750 ampD 2727790..2728347 (-) 558 WP_137373384.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  OCU78_RS11755 nadC 2728482..2729372 (+) 891 WP_137373383.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OCU78_RS11760 - 2729623..2730078 (+) 456 WP_137373382.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  OCU78_RS11765 pilB 2730080..2731765 (+) 1686 WP_137373381.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OCU78_RS11770 pilC 2731863..2733089 (+) 1227 WP_137373380.1 type II secretion system F family protein Machinery gene
  OCU78_RS11775 pilD 2733158..2734027 (+) 870 WP_137373379.1 A24 family peptidase Machinery gene
  OCU78_RS11780 coaE 2734030..2734644 (+) 615 WP_137373378.1 dephospho-CoA kinase -
  OCU78_RS11785 zapD 2734671..2735411 (+) 741 WP_137373377.1 cell division protein ZapD -
  OCU78_RS11790 yacG 2735474..2735671 (+) 198 WP_137373376.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 61966.77 Da        Isoelectric Point: 5.2032

>NTDB_id=92056 OCU78_RS11765 WP_137373381.1 2730080..2731765(+) (pilB) [Vibrio gallaecicus strain CECT 7244]
MSNNLPTLLLQANLLSLTQEQAVVEHIQASGASAPEALISLGCFNDKDLTAHLNEIFGLPVVQLSGIDYQPLCEQLGLRE
LITKYNAIPLRCSSSTLTLAVSDPTNLQAEDDFRFATGLQVEMVLANSDELIGAVRRLYGRSISGSNSKLKEISQDELAN
LVEVSDDEVTSIEDLSKDDSPVSRFINQILVDAVRKGASDIHFEPYEESYRVRLRCDGILVETQQPASQLSRRLSARLKI
LSKLDISERRLPQDGRIKLRLNEEIAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSDEQKQLYLNALQRPQGMI
LMTGPTGSGKTVSLYTGLRLLNTPERNISTAEDPVEINLSGINQVQVTPKIGFGFAEALRSFLRQDPDIVMVGEIRDLET
AEIAIKASQTGHLVLSTLHTNSAAETVARLSNMGIEPFNLASSLSLIIAQRLARRLCKHCKTPDTSPHIFLKHGIANEHT
IYQANKPGCNECTKGYSGRVGIYEVMPFSDDLKESLITKPNALEIEQIAKKNGMKTLQESGIQKLIEGTTSYQELQRVLY
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=92056 OCU78_RS11765 WP_137373381.1 2730080..2731765(+) (pilB) [Vibrio gallaecicus strain CECT 7244]
GTGTCCAATAACCTTCCAACACTGCTACTTCAGGCTAATTTACTTAGCCTGACGCAGGAGCAAGCTGTGGTGGAACATAT
ACAAGCTTCTGGCGCTTCTGCGCCAGAAGCTTTAATTTCTCTTGGTTGTTTTAACGATAAAGATCTCACTGCGCATTTAA
ACGAGATATTTGGTCTACCTGTCGTTCAGCTGTCAGGTATAGACTACCAGCCTCTATGTGAACAGCTTGGTTTACGTGAA
CTGATTACCAAGTACAATGCAATTCCTCTCAGGTGCTCATCATCAACGCTAACCCTAGCTGTTTCTGACCCAACAAACTT
ACAAGCCGAAGACGACTTTAGGTTTGCAACAGGCTTGCAAGTTGAAATGGTACTGGCTAATTCAGATGAGCTCATTGGTG
CTGTACGCCGTTTATACGGGCGGAGTATTTCCGGCTCTAATTCAAAACTCAAAGAAATCAGTCAAGATGAACTAGCAAAC
CTCGTTGAAGTCTCAGATGACGAAGTCACATCGATAGAAGATTTAAGTAAAGATGACTCTCCCGTTAGCCGATTTATTAA
CCAAATATTAGTCGATGCCGTGAGAAAAGGCGCATCAGATATTCATTTTGAACCTTACGAAGAGTCTTACCGCGTTAGGT
TGCGTTGCGACGGTATTCTTGTCGAAACTCAGCAGCCAGCCTCTCAGCTTAGCCGTCGCCTATCAGCTCGCTTAAAAATC
CTTTCTAAGCTGGATATATCGGAACGAAGACTCCCGCAAGATGGTCGTATTAAATTACGCCTGAATGAAGAAATAGCCAT
TGATATGCGCGTATCAACGCTGCCAACGCTATGGGGTGAGAAGATCGTCCTTCGCTTGTTAGATAGCAGTGCGGCCAATC
TCGATATTGATAAATTAGGCTACAGCGATGAACAAAAGCAGCTCTACTTAAATGCATTGCAACGCCCACAAGGTATGATT
TTGATGACAGGTCCAACGGGTAGCGGAAAAACCGTCTCACTTTATACAGGGCTTAGACTTCTCAATACGCCAGAAAGAAA
TATATCTACAGCCGAAGATCCTGTAGAGATCAACTTATCCGGTATCAACCAAGTTCAAGTTACCCCTAAGATTGGCTTTG
GCTTTGCAGAAGCCTTACGATCTTTTTTAAGGCAAGATCCAGACATCGTAATGGTTGGTGAAATACGAGATCTAGAAACC
GCGGAAATCGCAATTAAAGCCTCTCAGACAGGTCACTTAGTTTTATCTACTTTGCATACTAACTCAGCAGCTGAAACGGT
AGCCCGACTTTCCAATATGGGTATAGAACCTTTTAATCTTGCTTCTTCTTTAAGTCTCATCATTGCACAAAGACTTGCTC
GACGATTGTGCAAACATTGTAAAACTCCAGATACCTCACCTCATATATTTTTAAAACATGGAATAGCTAATGAGCACACC
ATTTATCAAGCCAATAAACCTGGCTGTAATGAATGCACCAAGGGCTACTCAGGTCGTGTCGGTATCTATGAGGTCATGCC
TTTTAGTGATGACTTAAAAGAAAGCTTAATCACAAAGCCAAACGCACTAGAAATAGAACAAATAGCGAAAAAAAACGGCA
TGAAAACTTTACAAGAATCCGGTATTCAAAAGCTTATTGAAGGCACAACTAGCTACCAAGAACTACAGCGTGTTTTGTAT
TTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

74.245

100

0.745
  pilB Vibrio campbellii strain DS40M4

72.42

100

0.725
  pilB Vibrio parahaemolyticus RIMD 2210633

72.42

100

0.725
  pilB Legionella pneumophila strain ERS1305867

51.158

92.335

0.472
  pilB Acinetobacter baumannii D1279779

51.663

91.087

0.471
  pilB Acinetobacter baylyi ADP1

51.874

90.374

0.469
  pilF Neisseria gonorrhoeae MS11

44.504

100

0.447
  pilB/pilB1 Synechocystis sp. PCC 6803

35.5

100

0.38
  pilF Thermus thermophilus HB27

38.309

99.109

0.38

Multiple sequence alignment