Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   QQK52_RS01965 Genome accession   NZ_AP027963
Coordinates   411275..411952 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain VE-T9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 406275..416952
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QQK52_RS01950 (VET9_03810) chiA 407633..410335 (+) 2703 WP_284345293.1 bifunctional chitinase/lysozyme -
  QQK52_RS01955 (VET9_03820) bfd 410504..410698 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  QQK52_RS01960 (VET9_03830) bfr 410770..411246 (+) 477 WP_000675504.1 bacterioferritin -
  QQK52_RS01965 (VET9_03840) pilD 411275..411952 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  QQK52_RS01970 (VET9_03850) gspM 411952..412413 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  QQK52_RS01975 (VET9_03860) gspL 412410..413573 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  QQK52_RS01980 (VET9_03870) gspK 413588..414571 (-) 984 WP_284345294.1 type II secretion system minor pseudopilin GspK -
  QQK52_RS01985 (VET9_03880) gspJ 414564..415151 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  QQK52_RS01990 (VET9_03890) gspI 415144..415521 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  QQK52_RS01995 (VET9_03900) gspH 415518..416027 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  QQK52_RS02000 (VET9_03910) gspG 416035..416472 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=92056 QQK52_RS01965 WP_000178161.1 411275..411952(-) (pilD) [Escherichia coli strain VE-T9]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=92056 QQK52_RS01965 WP_000178161.1 411275..411952(-) (pilD) [Escherichia coli strain VE-T9]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378

Multiple sequence alignment