Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   VK847_RS02545 Genome accession   NZ_CP141899
Coordinates   539080..540411 (+) Length   443 a.a.
NCBI ID   WP_095039669.1    Uniprot ID   A0A9Q5AXW3
Organism   Pseudomonas fragi strain LKYC.ZH     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 534080..545411
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VK847_RS02525 (VK847_02525) pilM 536432..537358 (+) 927 WP_324708433.1 type IV pilus assembly protein PilM -
  VK847_RS02530 (VK847_02530) - 537358..537924 (+) 567 WP_016783186.1 PilN domain-containing protein -
  VK847_RS02535 (VK847_02535) - 537924..538544 (+) 621 WP_324708434.1 type 4a pilus biogenesis protein PilO -
  VK847_RS02540 (VK847_02540) - 538541..539068 (+) 528 WP_324708435.1 pilus assembly protein PilP -
  VK847_RS02545 (VK847_02545) pilQ 539080..540411 (+) 1332 WP_095039669.1 type IV pilus secretin PilQ Machinery gene
  VK847_RS02550 (VK847_02550) aroK 540416..540934 (+) 519 WP_029611693.1 shikimate kinase AroK -
  VK847_RS02555 (VK847_02555) aroB 541091..542191 (+) 1101 WP_016783191.1 3-dehydroquinate synthase -
  VK847_RS02560 (VK847_02560) - 542202..543773 (+) 1572 WP_016783192.1 AAA family ATPase -

Sequence

Protein


Download         Length: 443 a.a.        Molecular weight: 47553.56 Da        Isoelectric Point: 5.1760

>NTDB_id=920430 VK847_RS02545 WP_095039669.1 539080..540411(+) (pilQ) [Pseudomonas fragi strain LKYC.ZH]
MKRISPVCVLALWSALNSQATLAAGQAAPSSIASAQYVGEPMSLNVQNLEVRTVLQSLADFNGFNLMVSDTVKGSITLSL
QDVPWDQALALVLGSKGLGKRLEGNVLWVAPLDEIAARERPEPAPLRRELVQVNYARAADIVMLFQSVTGGQAGLEGQGT
ITVDERTNNIIAFESSERLDELRRIVAQLDVPVRQVMIEAWIVEAGVDFEKSLGVRWGGASSNSGLWSASGITQPLQSGA
APGNASAPFVDLGVSRNSSGLGIAFVTNNVLLDLELTAMEKTGNGEIISQPKVVTADKETAKILKGTQIPYQQASKTGAT
SVSFKEASLSLEVTPQITPDNSVIMAVVVTKDEPDYMSRLQDVPPIKKNEVRAKVLVKDGETIVIGGVFSKTQSKVVDKV
PFLGDLPYVGRLFHRDVVLEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 1332 bp        

>NTDB_id=920430 VK847_RS02545 WP_095039669.1 539080..540411(+) (pilQ) [Pseudomonas fragi strain LKYC.ZH]
ATGAAAAGGATTTCACCGGTCTGCGTACTGGCGCTATGGAGTGCGCTGAATTCGCAGGCAACACTGGCGGCCGGACAGGC
CGCGCCCTCATCCATCGCGAGTGCGCAGTATGTGGGCGAACCCATGTCCCTGAACGTGCAGAACCTTGAAGTACGCACGG
TGCTGCAATCGCTGGCGGATTTTAACGGGTTCAACCTGATGGTCAGCGATACCGTCAAAGGCAGTATCACCCTCAGTTTG
CAGGACGTGCCTTGGGATCAGGCGCTGGCTCTGGTGCTTGGCAGCAAGGGCCTGGGCAAGCGCCTTGAGGGTAATGTGCT
GTGGGTCGCGCCCCTGGACGAGATCGCCGCCCGCGAGCGGCCAGAGCCTGCGCCTTTGCGTCGGGAGCTGGTGCAGGTCA
ATTACGCCAGGGCCGCAGATATCGTCATGCTGTTCCAGTCAGTGACCGGCGGTCAGGCCGGTCTCGAGGGGCAGGGCACG
ATCACGGTAGATGAGCGCACCAATAACATCATTGCCTTTGAATCCAGTGAACGGCTGGATGAGCTGCGGCGCATCGTGGC
CCAACTGGACGTGCCGGTGCGCCAGGTGATGATTGAGGCCTGGATTGTCGAGGCGGGAGTGGACTTCGAAAAAAGCCTGG
GTGTTCGCTGGGGTGGGGCATCAAGCAACAGCGGCCTGTGGAGTGCCAGCGGCATCACCCAGCCATTGCAGTCAGGGGCT
GCGCCCGGCAACGCCAGCGCGCCTTTTGTCGACCTGGGGGTTTCGCGCAACTCATCGGGGTTGGGCATCGCCTTTGTGAC
CAACAATGTGCTGCTGGACCTGGAGCTGACGGCCATGGAAAAAACCGGTAATGGCGAAATCATCTCGCAGCCCAAGGTGG
TTACCGCAGACAAGGAAACCGCAAAAATCCTCAAAGGCACGCAAATTCCCTATCAACAGGCCAGCAAAACCGGTGCTACA
TCGGTCTCGTTCAAGGAGGCCTCGCTGTCGCTGGAAGTCACGCCGCAAATCACCCCGGACAACAGTGTCATCATGGCCGT
CGTAGTGACCAAGGATGAGCCTGATTACATGAGCCGGTTGCAGGATGTGCCGCCCATCAAGAAGAACGAGGTCAGGGCCA
AGGTGTTGGTCAAGGATGGCGAAACCATCGTGATTGGGGGGGTGTTCTCAAAAACTCAAAGCAAAGTGGTAGATAAAGTG
CCATTTTTGGGCGATCTGCCGTATGTTGGCCGACTTTTCCATAGAGACGTTGTCCTGGAAAAAAAATCCGAACTGCTGGT
GTTCCTGACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCATTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(130-195)

Bacterial type II and III secretion system protein (Secretin)

(278-434)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

64.76

98.646

0.639
  comQ Acinetobacter baylyi ADP1

42.411

100

0.429
  pilQ Acinetobacter baumannii strain A118

39.362

100

0.418
  pilQ Acinetobacter baumannii D1279779

39.362

100

0.418
  pilQ Vibrio campbellii strain DS40M4

40.222

100

0.409
  pilQ Legionella pneumophila strain Lp02

40.826

98.42

0.402
  pilQ Legionella pneumophila strain ERS1305867

40.596

98.42

0.4
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.486

96.614

0.381
  pilQ Vibrio cholerae strain A1552

39.486

96.614

0.381