Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OCV50_RS11820 Genome accession   NZ_AP025487
Coordinates   2723486..2725171 (+) Length   561 a.a.
NCBI ID   WP_261903137.1    Uniprot ID   -
Organism   Vibrio fortis strain LMG 21557     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2718486..2730171
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV50_RS11800 pdhR 2720001..2720768 (-) 768 WP_239841201.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  OCV50_RS11805 ampD 2721217..2721765 (-) 549 WP_261903134.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  OCV50_RS11810 nadC 2721914..2722804 (+) 891 WP_261903135.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OCV50_RS11815 pilA 2723058..2723477 (+) 420 WP_261903136.1 pilin Machinery gene
  OCV50_RS11820 pilB 2723486..2725171 (+) 1686 WP_261903137.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OCV50_RS11825 pilC 2725199..2726428 (+) 1230 WP_261903138.1 type II secretion system F family protein Machinery gene
  OCV50_RS11830 pilD 2726488..2727357 (+) 870 WP_261903139.1 A24 family peptidase Machinery gene
  OCV50_RS11835 coaE 2727361..2727969 (+) 609 WP_261903140.1 dephospho-CoA kinase -
  OCV50_RS11840 zapD 2728002..2728742 (+) 741 WP_239841209.1 cell division protein ZapD -
  OCV50_RS11845 yacG 2728814..2729011 (+) 198 WP_239841210.1 DNA gyrase inhibitor YacG -
  OCV50_RS11850 rplS 2729452..2729805 (-) 354 WP_032549758.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62364.94 Da        Isoelectric Point: 5.4722

>NTDB_id=92009 OCV50_RS11820 WP_261903137.1 2723486..2725171(+) (pilB) [Vibrio fortis strain LMG 21557]
MLTNLPTVLRQANQLSLTQEQVVEEHVQASGGSTPEALIAHGFLNAETLAEHTSHIFGLPLISLADYDYELLCEQLGLRE
LITQYHAIPVAKSASTLTLASADPTDLQAEDDFRFATGLQVELVVANYRTLEGAIRRLYGKAITHGESNRKEITQDELAH
LVEVSDDEVSSVEDLSQDESPVSRFINQILVDAVRKGASDIHFEPYEEYYRVRLRCDGILVETQQPAAHLSRRLSARLKI
LAKLDIAERRLPQDGRIKLRLNQEIAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDILGYSEQQKTLYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRILNTSERNISTAEDPVEINLCGVNQVQVQPKIGFGFAEALRSFLRQDPDIVMVGEIRDIET
AEIAIKASQTGHLVLSTLHTNSAAETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCPHCKMADDDQTIYHHHDISAHHT
FYKANPNGCNECNQGYSGRVGIYEVMPFTEKLKASLISRPNAIEIENLAKQEGMQTLRDAGLEKLLSGVTSYQELQRVLY
I

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=92009 OCV50_RS11820 WP_261903137.1 2723486..2725171(+) (pilB) [Vibrio fortis strain LMG 21557]
ATGCTAACCAATCTCCCCACAGTTCTACGTCAGGCTAACCAGCTTAGTCTGACTCAAGAACAAGTTGTCGAGGAACATGT
GCAAGCTTCTGGTGGTTCAACCCCAGAAGCTTTGATTGCTCACGGTTTCTTGAACGCAGAAACCCTTGCCGAACACACTT
CTCATATCTTCGGCTTACCTTTGATTTCGCTAGCTGACTACGACTACGAGCTGTTGTGTGAACAGCTTGGGCTGCGCGAA
CTTATCACCCAATATCATGCAATACCCGTCGCAAAATCAGCGTCAACACTCACGTTAGCCTCCGCCGATCCAACCGACCT
ACAAGCCGAAGACGATTTTCGTTTTGCTACTGGGCTGCAAGTTGAGCTAGTGGTAGCAAACTATCGCACACTGGAAGGTG
CTATTCGGCGACTTTATGGCAAAGCGATTACTCATGGCGAGAGCAATCGTAAAGAGATCACGCAGGATGAACTCGCTCAC
TTGGTGGAGGTTTCTGATGATGAAGTGAGTAGCGTCGAAGACCTTAGCCAAGACGAATCGCCCGTCAGTCGCTTTATCAA
TCAAATCCTAGTTGATGCTGTGCGCAAAGGGGCGTCTGATATCCATTTCGAGCCCTATGAAGAGTACTACCGAGTTCGCC
TGCGCTGTGATGGCATCTTAGTCGAAACCCAACAGCCCGCAGCCCACCTGAGCCGGAGACTCTCAGCACGCCTTAAAATT
CTAGCCAAGCTTGATATTGCTGAGCGACGTTTGCCTCAAGACGGTCGAATTAAGTTAAGACTCAATCAAGAGATTGCCAT
TGATATGCGCGTCTCTACCTTGCCAACACTGTGGGGCGAGAAGATTGTGCTTCGCCTACTCGATAGCAGTGCAGCCAATC
TTGATATCGACATCCTTGGTTACAGTGAACAACAAAAGACACTCTACCTGAATGCTCTGAAACGCCCACAAGGCATGATT
CTTATGACTGGGCCGACAGGTAGTGGCAAAACCGTATCGCTCTACACAGGCCTTCGTATTCTTAACACCTCGGAAAGAAA
CATCTCCACTGCCGAAGACCCGGTTGAGATTAACCTATGTGGTGTCAATCAAGTGCAAGTACAGCCCAAAATAGGCTTTG
GGTTTGCTGAGGCGCTACGCTCTTTTCTACGCCAAGACCCTGATATCGTAATGGTTGGTGAAATTCGTGATATTGAAACC
GCCGAAATAGCGATTAAAGCCTCTCAAACGGGGCACTTAGTTCTTTCAACACTTCACACCAACTCTGCAGCGGAAACTGT
CACGCGCCTTGCACACATGGGAATAGAACCCTTTAATCTCGCGTCATCATTAAGCCTCATCATCGCTCAACGCCTCGCTC
GACGACTATGCCCGCACTGCAAAATGGCAGACGATGACCAAACCATTTATCACCATCATGACATCAGTGCTCACCATACC
TTTTACAAAGCAAACCCAAACGGTTGCAACGAGTGCAATCAAGGCTATTCCGGACGCGTTGGTATATATGAAGTCATGCC
ATTCACCGAAAAGCTCAAAGCCAGTCTAATCTCTCGTCCAAATGCTATCGAGATAGAAAACTTAGCTAAGCAGGAAGGTA
TGCAAACTCTACGAGACGCAGGGTTAGAGAAACTGCTATCAGGTGTGACTAGCTATCAAGAGCTGCAACGTGTTCTCTAT
ATCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

72.954

100

0.731
  pilB Vibrio parahaemolyticus RIMD 2210633

70.945

100

0.709
  pilB Vibrio campbellii strain DS40M4

70.41

100

0.704
  pilB Acinetobacter baumannii D1279779

50.282

94.652

0.476
  pilB Acinetobacter baylyi ADP1

49.527

94.296

0.467
  pilB Legionella pneumophila strain ERS1305867

49.245

94.474

0.465
  pilF Neisseria gonorrhoeae MS11

43.964

98.93

0.435
  pilF Thermus thermophilus HB27

38.42

99.287

0.381
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.989

95.722

0.364

Multiple sequence alignment