Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA/celA/cilE   Type   Machinery gene
Locus tag   VNN28_RS01510 Genome accession   NZ_CP141725
Coordinates   302874..303515 (-) Length   213 a.a.
NCBI ID   WP_165711419.1    Uniprot ID   -
Organism   Lactococcus formosensis strain M03102409 EE5d7     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 297874..308515
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VNN28_RS01485 (VNN28_01485) atpF 298014..298520 (-) 507 WP_017368854.1 F0F1 ATP synthase subunit B -
  VNN28_RS01490 (VNN28_01490) atpB 298534..299247 (-) 714 WP_338997737.1 F0F1 ATP synthase subunit A -
  VNN28_RS01495 (VNN28_01495) - 299302..299517 (-) 216 WP_003135074.1 F0F1 ATP synthase subunit C -
  VNN28_RS01500 (VNN28_01500) - 299713..300489 (-) 777 WP_185767510.1 alpha/beta hydrolase family protein -
  VNN28_RS01505 (VNN28_01505) comEC 300644..302854 (-) 2211 WP_338997738.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  VNN28_RS01510 (VNN28_01510) comEA/celA/cilE 302874..303515 (-) 642 WP_165711419.1 helix-hairpin-helix domain-containing protein Machinery gene
  VNN28_RS01515 (VNN28_01515) - 303573..304748 (-) 1176 WP_017368849.1 SLC13 family permease -
  VNN28_RS01520 (VNN28_01520) - 304913..306469 (-) 1557 WP_017368848.1 ABC-F family ATP-binding cassette domain-containing protein -
  VNN28_RS01525 (VNN28_01525) sufB 306768..308165 (-) 1398 WP_165711421.1 Fe-S cluster assembly protein SufB -

Sequence

Protein


Download         Length: 213 a.a.        Molecular weight: 23579.75 Da        Isoelectric Point: 5.1049

>NTDB_id=918972 VNN28_RS01510 WP_165711419.1 302874..303515(-) (comEA/celA/cilE) [Lactococcus formosensis strain M03102409 EE5d7]
MKKLTEYINENLKTIVGVVLALTILGIFYWNKKVPDLPKKEEDLPEWVEGSKEKDGSEKKNTEKVDEDEIIVDVKGAVKK
PGIYKLSSKNRVSDAVASAGGFTEEAERKGINLAKKLQDEAEVYVPLRGEADTNHLLENTENTNKTAKKAKININTADLT
ELQQLSGVGAKKAQDILDYRSENGSFQTVEELTKVSGFGPKTLEKLKEAITVD

Nucleotide


Download         Length: 642 bp        

>NTDB_id=918972 VNN28_RS01510 WP_165711419.1 302874..303515(-) (comEA/celA/cilE) [Lactococcus formosensis strain M03102409 EE5d7]
ATGAAAAAACTAACAGAATATATAAATGAAAATTTAAAAACTATTGTTGGAGTTGTTTTAGCATTGACTATTTTAGGTAT
ATTTTACTGGAATAAAAAAGTTCCTGATCTGCCTAAGAAAGAAGAAGATTTACCTGAGTGGGTAGAGGGAAGCAAGGAAA
AAGACGGTTCAGAGAAGAAAAATACTGAAAAGGTAGATGAGGATGAAATCATTGTTGATGTTAAAGGCGCTGTTAAAAAA
CCTGGTATCTACAAATTGTCTTCAAAAAACCGTGTATCCGATGCAGTAGCAAGTGCTGGTGGTTTTACGGAAGAAGCGGA
GAGAAAAGGAATAAATCTGGCAAAAAAGTTGCAAGATGAAGCTGAAGTTTATGTTCCTTTGCGAGGGGAAGCAGATACAA
ATCACTTGTTAGAAAACACAGAAAATACCAACAAAACCGCCAAAAAAGCAAAAATTAATATTAATACAGCAGATTTAACT
GAATTGCAGCAACTTAGTGGTGTGGGAGCAAAAAAAGCACAAGATATACTGGATTACCGTTCTGAAAATGGTTCCTTTCA
GACAGTGGAAGAATTGACTAAGGTGAGTGGATTTGGCCCTAAAACATTAGAAAAATTGAAAGAAGCAATCACAGTAGATT
AG

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(149-211)

SLBB domain (SLBB)

(71-125)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA/celA/cilE Streptococcus mitis SK321

47.032

100

0.484
  comEA/celA/cilE Streptococcus mitis NCTC 12261

45.662

100

0.469
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

44.749

100

0.46
  comEA/celA/cilE Streptococcus pneumoniae Rx1

43.836

100

0.451
  comEA/celA/cilE Streptococcus pneumoniae D39

43.836

100

0.451
  comEA/celA/cilE Streptococcus pneumoniae R6

43.836

100

0.451
  comEA Lactococcus lactis subsp. cremoris KW2

43.243

100

0.451
  comEA Latilactobacillus sakei subsp. sakei 23K

36.937

100

0.385
  comEA Staphylococcus aureus MW2

35.616

100

0.366