Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   VNN32_RS03540 Genome accession   NZ_CP141703
Coordinates   659662..660156 (-) Length   164 a.a.
NCBI ID   WP_017369521.1    Uniprot ID   A0A9Q9D6U2
Organism   Lactococcus petauri strain R21-74 AL-1     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 641863..665140 659662..660156 within 0


Gene organization within MGE regions


Location: 641863..665140
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VNN32_RS03390 (VNN32_03390) - 641863..642186 (-) 324 WP_338976297.1 sigma factor-like helix-turn-helix DNA-binding protein -
  VNN32_RS03395 (VNN32_03395) - 642252..642422 (-) 171 WP_338976146.1 hypothetical protein -
  VNN32_RS03400 (VNN32_03400) - 642645..642923 (-) 279 WP_338976144.1 hypothetical protein -
  VNN32_RS03405 (VNN32_03405) - 642931..643287 (-) 357 WP_338976221.1 hypothetical protein -
  VNN32_RS03410 (VNN32_03410) - 643287..643808 (-) 522 WP_338976223.1 hypothetical protein -
  VNN32_RS03415 (VNN32_03415) - 644001..644180 (-) 180 WP_014025437.1 hypothetical protein -
  VNN32_RS03420 (VNN32_03420) - 644237..644749 (-) 513 WP_338976225.1 hypothetical protein -
  VNN32_RS03425 (VNN32_03425) - 645233..646948 (-) 1716 WP_338976299.1 hypothetical protein -
  VNN32_RS03430 (VNN32_03430) - 646945..647274 (-) 330 WP_003133226.1 HTH domain-containing protein -
  VNN32_RS03435 (VNN32_03435) - 647271..647462 (-) 192 WP_017371269.1 hypothetical protein -
  VNN32_RS03440 (VNN32_03440) - 647660..647941 (-) 282 WP_003133227.1 hypothetical protein -
  VNN32_RS03445 (VNN32_03445) - 647925..648089 (-) 165 WP_014025432.1 hypothetical protein -
  VNN32_RS03450 (VNN32_03450) - 648235..648417 (-) 183 WP_311842852.1 DUF1655 domain-containing protein -
  VNN32_RS03455 (VNN32_03455) - 648417..648710 (-) 294 WP_338976301.1 hypothetical protein -
  VNN32_RS03460 (VNN32_03460) - 648813..649073 (-) 261 WP_338976303.1 hypothetical protein -
  VNN32_RS03465 (VNN32_03465) - 649116..649322 (-) 207 WP_004259899.1 XRE family transcriptional regulator -
  VNN32_RS03470 (VNN32_03470) - 649615..650004 (+) 390 WP_338976305.1 helix-turn-helix domain-containing protein -
  VNN32_RS03475 (VNN32_03475) - 650014..650568 (+) 555 WP_338976307.1 hypothetical protein -
  VNN32_RS03480 (VNN32_03480) - 650568..650750 (+) 183 WP_338976309.1 hypothetical protein -
  VNN32_RS03490 (VNN32_03490) - 651856..653040 (+) 1185 WP_338976311.1 site-specific integrase -
  VNN32_RS03495 (VNN32_03495) - 653252..653644 (-) 393 WP_019335765.1 VOC family protein -
  VNN32_RS03500 (VNN32_03500) - 653653..654030 (-) 378 WP_019335764.1 helix-turn-helix domain-containing protein -
  VNN32_RS03505 (VNN32_03505) - 654172..654990 (+) 819 WP_046401025.1 aldo/keto reductase -
  VNN32_RS03510 (VNN32_03510) - 655037..656188 (+) 1152 WP_338976316.1 MFS transporter -
  VNN32_RS03515 (VNN32_03515) recX 656283..657116 (+) 834 WP_046401051.1 recombination regulator RecX -
  VNN32_RS03520 (VNN32_03520) - 657199..658047 (+) 849 WP_019291952.1 MurR/RpiR family transcriptional regulator -
  VNN32_RS03525 (VNN32_03525) - 658079..658387 (+) 309 WP_019291953.1 DUF960 domain-containing protein -
  VNN32_RS03530 (VNN32_03530) - 658421..659188 (-) 768 WP_046401027.1 metallophosphoesterase -
  VNN32_RS03535 (VNN32_03535) rpsR 659297..659542 (-) 246 WP_003133134.1 30S ribosomal protein S18 -
  VNN32_RS03540 (VNN32_03540) ssb 659662..660156 (-) 495 WP_017369521.1 single-stranded DNA-binding protein Machinery gene
  VNN32_RS03545 (VNN32_03545) rpsF 660194..660487 (-) 294 WP_046401028.1 30S ribosomal protein S6 -
  VNN32_RS03550 (VNN32_03550) - 660673..660885 (-) 213 WP_019291955.1 hypothetical protein -
  VNN32_RS03555 (VNN32_03555) - 661352..662815 (+) 1464 WP_019291956.1 amino acid permease -
  VNN32_RS03560 (VNN32_03560) dnaX 662849..664507 (-) 1659 WP_019335759.1 DNA polymerase III subunit gamma/tau -
  VNN32_RS03565 (VNN32_03565) - 664520..665140 (-) 621 WP_046401029.1 hypothetical protein -

Sequence


Protein


Download         Length: 164 a.a.        Molecular weight: 18019.88 Da        Isoelectric Point: 4.9533

>NTDB_id=918572 VNN32_RS03540 WP_017369521.1 659662..660156(-) (ssb) [Lactococcus petauri strain R21-74 AL-1]
MINNVVLVGRVVRDPELRYTPQNTAVATFTIAVNRRFKNAQGEREADFINCVIWRQSAENLANWAKKGALIGVTGSIQVR
NYENKEGQRVYVTEVLADNFQMLESRAAREGGSGSYSAPQQQQSQNRPFAGSNSSAGNSTPDFGRDADPFGGSPMEISDD
DLPF

Nucleotide


Download         Length: 495 bp        

>NTDB_id=918572 VNN32_RS03540 WP_017369521.1 659662..660156(-) (ssb) [Lactococcus petauri strain R21-74 AL-1]
ATGATTAATAATGTTGTTTTGGTCGGACGCGTTGTGCGTGACCCTGAACTGCGATACACACCGCAAAATACTGCGGTTGC
TACATTTACGATTGCTGTTAACCGTCGTTTTAAAAACGCGCAAGGCGAACGTGAAGCTGATTTCATTAACTGTGTCATCT
GGCGTCAATCTGCTGAAAACTTGGCAAATTGGGCTAAAAAAGGTGCCTTGATTGGTGTAACAGGTAGTATTCAAGTGCGA
AATTATGAAAACAAAGAAGGTCAACGTGTTTACGTGACCGAAGTATTGGCTGATAACTTCCAAATGCTTGAAAGTCGTGC
CGCACGTGAAGGTGGCTCTGGTTCATATTCTGCACCACAGCAACAACAATCACAAAATCGTCCTTTTGCTGGCTCTAACA
GCTCAGCAGGAAATTCAACACCTGATTTTGGTCGTGATGCAGATCCATTTGGTGGCTCACCAATGGAAATCTCAGATGAT
GATCTTCCATTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

56.725

100

0.591

  ssbA Bacillus subtilis subsp. subtilis str. 168

54.494

100

0.591

  ssbB/cilA Streptococcus pneumoniae TIGR4

49.242

80.488

0.396

  ssbB/cilA Streptococcus pneumoniae D39

48.485

80.488

0.39

  ssbB/cilA Streptococcus pneumoniae R6

48.485

80.488

0.39

  ssbB/cilA Streptococcus mitis SK321

48.485

80.488

0.39

  ssbB/cilA Streptococcus mitis NCTC 12261

48.485

80.488

0.39

  ssbB/cilA Streptococcus pneumoniae Rx1

48.485

80.488

0.39

  ssbB Bacillus subtilis subsp. subtilis str. 168

57.547

64.634

0.372