Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OCV39_RS02530 Genome accession   NZ_AP025472
Coordinates   514806..516485 (-) Length   559 a.a.
NCBI ID   WP_261888863.1    Uniprot ID   -
Organism   Vibrio cortegadensis strain CECT 7227     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 509806..521485
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV39_RS02500 rplS 510394..510747 (+) 354 WP_113799265.1 50S ribosomal protein L19 -
  OCV39_RS02505 yacG 510862..511059 (-) 198 WP_017054173.1 DNA gyrase inhibitor YacG -
  OCV39_RS02510 zapD 511094..511834 (-) 741 WP_017054172.1 cell division protein ZapD -
  OCV39_RS02515 coaE 511862..512476 (-) 615 WP_261888860.1 dephospho-CoA kinase -
  OCV39_RS02520 pilD 512478..513347 (-) 870 WP_261888861.1 A24 family peptidase Machinery gene
  OCV39_RS02525 pilC 513443..514669 (-) 1227 WP_261888862.1 type II secretion system F family protein Machinery gene
  OCV39_RS02530 pilB 514806..516485 (-) 1680 WP_261888863.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OCV39_RS02535 pilA 516486..516896 (-) 411 WP_261888864.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  OCV39_RS02540 nadC 517154..518044 (-) 891 WP_261888865.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OCV39_RS02545 ampD 518184..518732 (+) 549 WP_261889459.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  OCV39_RS02550 pdhR 519200..519967 (+) 768 WP_017054164.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62369.93 Da        Isoelectric Point: 5.7160

>NTDB_id=91829 OCV39_RS02530 WP_261888863.1 514806..516485(-) (pilB) [Vibrio cortegadensis strain CECT 7227]
MFTNLPAILRQANLLSLTQEQTLVEHMNASGLSVPEALFDLGLFSANTLTEHLSAIFDLPETNLDHYDYHDLCQKLGLRE
LITRHQALPISSHAHVLTIAVSDPTHLLIEDDFRFATGHQVELVLANFKTLQGAIRRLYGHSIQGAQQTQKEISQEELAN
LIEVSEDEIENIEDLSQDDSPVSRFINQVLLDAVRKKASDIHFEPYEDNYRIRLRCDGILVETQQPASHLSRRLSARLKI
LAKLDISERRLPQDGRIKLRLNQDNALDLRVSSLPTLWGEKIVLRLLDSSAANLDIDTLGYNSEQKQLYLNALKKPQGMI
LMTGPTGSGKTVSLYTGLRILNTSEVNISTAEDPVEINLSGINQVQVKPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVVRLTNMGIEAFNLASSLSLIIAQRLARRLCEHCRQPDTNTPLHLEISAEDRIY
RANPKGCSECTGGYSGRVGIYEVMEFNSDLSEALIKGASINQIEQLAQKNGMQNLHNSGIEKLKQGITSYRELQRVLYF

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=91829 OCV39_RS02530 WP_261888863.1 514806..516485(-) (pilB) [Vibrio cortegadensis strain CECT 7227]
TTGTTCACCAACCTCCCCGCTATTCTACGTCAGGCTAACCTGCTTAGCCTGACGCAAGAACAAACACTGGTTGAACATAT
GAATGCTTCTGGCCTTTCGGTACCGGAGGCATTGTTTGATCTTGGCCTTTTTTCGGCCAACACCCTAACCGAGCACCTAA
GCGCTATCTTTGATTTACCTGAAACCAACCTTGACCATTATGACTACCATGACCTGTGCCAAAAACTTGGGTTGCGTGAA
CTGATCACCCGCCATCAAGCGTTGCCTATTTCCAGTCATGCCCATGTGCTCACCATTGCCGTATCTGACCCCACTCATTT
ATTAATCGAAGATGATTTTCGTTTTGCGACGGGTCACCAAGTTGAGTTAGTACTCGCGAACTTTAAAACACTGCAAGGGG
CGATTCGACGCTTGTATGGACATTCGATCCAAGGCGCTCAACAAACACAGAAAGAGATCAGCCAAGAAGAGCTAGCTAAC
TTAATTGAGGTCAGTGAAGATGAGATCGAAAATATTGAAGATCTTAGCCAAGATGACTCCCCTGTTAGCCGTTTTATCAA
TCAAGTGTTATTAGATGCCGTCCGTAAGAAAGCCTCCGATATTCACTTTGAACCTTACGAAGACAACTATCGCATTCGTC
TGCGTTGCGATGGCATTTTGGTTGAAACTCAACAACCCGCCAGTCATTTAAGCCGGAGGCTTTCAGCACGATTAAAAATT
CTCGCCAAACTCGATATTTCAGAACGACGCTTGCCTCAAGATGGGCGGATCAAGCTCAGGTTGAATCAAGATAACGCCCT
CGATCTCCGTGTCTCTAGCTTGCCAACATTATGGGGGGAAAAGATCGTATTACGGCTGCTCGATAGCAGTGCAGCCAACC
TCGATATCGACACGCTTGGCTATAATTCAGAACAAAAACAACTCTACCTTAACGCCTTAAAAAAGCCACAAGGCATGATT
TTAATGACCGGCCCAACAGGAAGTGGAAAAACGGTGTCACTCTATACTGGACTCAGAATTCTAAATACCAGCGAAGTGAA
CATTTCGACGGCGGAAGATCCTGTAGAGATCAATTTATCGGGGATCAACCAAGTTCAAGTCAAACCGAAAATTGGCTTTG
GTTTTGCTGAAGCTCTGCGATCTTTTCTCCGTCAAGATCCCGATGTGGTCATGGTTGGGGAGATCCGTGATTTAGAAACC
GCCGAAATAGCGGTGAAAGCAGCGCAAACTGGCCACTTGGTGCTTTCCACGCTTCACACCAACTCTGCCGCAGAGACCGT
AGTACGGCTTACTAATATGGGCATTGAAGCATTCAATCTCGCCTCATCTTTAAGTTTAATCATTGCTCAGCGTCTGGCTC
GAAGGCTGTGCGAGCATTGCCGTCAACCGGATACCAATACACCTCTACACCTTGAAATAAGTGCTGAAGATCGTATCTAT
CGAGCAAATCCTAAAGGTTGTAGCGAATGCACTGGCGGTTACTCTGGTCGAGTGGGTATTTATGAAGTGATGGAGTTTAA
CTCAGATCTTTCTGAAGCTCTTATTAAAGGAGCCAGCATTAATCAAATTGAGCAATTAGCGCAGAAGAACGGAATGCAGA
ATTTACACAACTCAGGGATAGAAAAATTAAAACAAGGCATCACTAGCTATCGTGAATTACAACGAGTGCTCTACTTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

76.512

100

0.769
  pilB Vibrio campbellii strain DS40M4

72.549

100

0.728
  pilB Vibrio parahaemolyticus RIMD 2210633

72.549

100

0.728
  pilB Acinetobacter baumannii D1279779

49.62

94.097

0.467
  pilB Acinetobacter baylyi ADP1

49.057

94.812

0.465
  pilB Legionella pneumophila strain ERS1305867

49.805

91.592

0.456
  pilF Neisseria gonorrhoeae MS11

45.035

100

0.454
  pilF Thermus thermophilus HB27

37.117

99.284

0.369

Multiple sequence alignment