Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   VNN43_RS03205 Genome accession   NZ_CP141685
Coordinates   643574..644518 (-) Length   314 a.a.
NCBI ID   WP_195916935.1    Uniprot ID   -
Organism   Lactococcus petauri strain R21-69 HC     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 638574..649518
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VNN43_RS03165 (VNN43_03165) - 639181..640065 (+) 885 WP_019336000.1 RluA family pseudouridine synthase -
  VNN43_RS03170 (VNN43_03170) - 640136..640981 (-) 846 WP_019336001.1 zinc ABC transporter substrate-binding protein -
  VNN43_RS03175 (VNN43_03175) comYF 641213..641479 (-) 267 WP_255566449.1 competence type IV pilus minor pilin ComGF Machinery gene
  VNN43_RS03180 (VNN43_03180) comGE 641634..641798 (-) 165 WP_019336003.1 competence system putative prepilin ComGE -
  VNN43_RS03185 (VNN43_03185) - 641771..641869 (-) 99 WP_255566393.1 hypothetical protein -
  VNN43_RS03190 (VNN43_03190) comGD 641901..642317 (-) 417 WP_019336004.1 competence type IV pilus minor pilin ComGD Machinery gene
  VNN43_RS03195 (VNN43_03195) comYC 642292..642597 (-) 306 WP_029343537.1 competence type IV pilus major pilin ComGC Machinery gene
  VNN43_RS03200 (VNN43_03200) comGB 642616..643635 (-) 1020 WP_255566448.1 competence type IV pilus assembly protein ComGB Machinery gene
  VNN43_RS03205 (VNN43_03205) comGA 643574..644518 (-) 945 WP_195916935.1 competence type IV pilus ATPase ComGA Machinery gene
  VNN43_RS03210 (VNN43_03210) rpsU 644704..644880 (-) 177 WP_003134539.1 30S ribosomal protein S21 -
  VNN43_RS03215 (VNN43_03215) guaB 645006..646487 (-) 1482 WP_003133714.1 IMP dehydrogenase -
  VNN43_RS03220 (VNN43_03220) - 646656..648257 (+) 1602 WP_019336007.1 GH25 family lysozyme -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 35753.13 Da        Isoelectric Point: 7.2081

>NTDB_id=918001 VNN43_RS03205 WP_195916935.1 643574..644518(-) (comGA) [Lactococcus petauri strain R21-69 HC]
MIQVLAKNLLQKAVDQMVHDLYLVALEGKYSLYFRTATERRFERELTLEQGQALIAHMKFLSGMNLGESRRVQLGACTYI
LDKGEQRLRLSTVGDFHGQESLVIRLLHHQQNQLHFWNSEIFNTIIGGRGLYLFSGPVGSGKTSLMYKFAREHFKNQQVI
CIEDPVELVETEFLQLQVNKVIGNDYDALIKLSLRHRPDLLIVGEIRDSQTAKAVLRASLTGYTVFSTVHARSISGVIAR
LKELGLSDWELQSSLQRVIYQRLIAGKGLLVCEKEKFEEWKPDEWNDQIDQLVADGFISAVAAAREKIEFSKAD

Nucleotide


Download         Length: 945 bp        

>NTDB_id=918001 VNN43_RS03205 WP_195916935.1 643574..644518(-) (comGA) [Lactococcus petauri strain R21-69 HC]
ATGATACAGGTTTTGGCAAAAAACCTTTTACAAAAAGCAGTAGATCAGATGGTGCACGATCTTTATTTAGTAGCATTAGA
AGGAAAGTATTCTCTCTATTTTCGTACAGCAACAGAAAGGAGGTTTGAAAGAGAACTCACCCTTGAGCAAGGGCAAGCAC
TGATTGCTCATATGAAATTTTTATCTGGTATGAATCTAGGAGAAAGTAGACGAGTACAGTTGGGGGCTTGCACTTATATC
TTAGATAAAGGTGAGCAGCGCTTACGTTTATCTACTGTCGGTGATTTTCATGGTCAAGAAAGCTTGGTTATTCGACTCTT
GCATCATCAACAAAACCAACTTCATTTTTGGAATTCAGAGATCTTTAATACTATTATTGGGGGAAGAGGCCTTTACCTTT
TTTCAGGTCCTGTAGGTTCAGGGAAGACCAGTCTTATGTACAAATTTGCAAGGGAGCATTTTAAAAATCAACAAGTTATT
TGTATAGAAGATCCTGTGGAGTTAGTAGAAACTGAATTTTTACAACTTCAAGTCAATAAAGTAATAGGGAATGATTATGA
TGCCTTGATTAAGCTTTCGCTGCGACACAGACCTGATTTACTTATTGTTGGTGAAATTAGGGATAGTCAAACGGCAAAAG
CTGTTCTCCGTGCAAGTTTGACTGGGTATACAGTATTCTCTACTGTTCATGCACGCTCAATATCAGGTGTAATTGCACGC
TTAAAAGAACTTGGATTAAGTGATTGGGAGCTACAATCCAGTCTTCAGCGGGTGATTTATCAACGATTAATTGCAGGAAA
GGGATTGCTTGTTTGTGAAAAGGAAAAGTTTGAAGAATGGAAACCAGACGAATGGAATGACCAGATTGATCAGTTGGTTG
CAGATGGATTTATCTCAGCTGTTGCAGCTGCGCGCGAAAAAATTGAGTTTAGCAAAGCAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

60.191

100

0.602
  comYA Streptococcus gordonii str. Challis substr. CH1

53.333

100

0.535
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

52.716

99.682

0.525
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

52.564

99.363

0.522
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

52.396

99.682

0.522
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

52.396

99.682

0.522
  comGA/cglA/cilD Streptococcus pneumoniae D39

52.396

99.682

0.522
  comGA/cglA/cilD Streptococcus pneumoniae R6

52.396

99.682

0.522
  comYA Streptococcus mutans UA159

50.949

100

0.513
  comYA Streptococcus mutans UA140

50.949

100

0.513