Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   U8518_RS21605 Genome accession   NZ_CP141267
Coordinates   4705824..4707539 (-) Length   571 a.a.
NCBI ID   WP_180807586.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain MaAh001     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4700824..4712539
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U8518_RS21575 (U8518_21575) mutT 4701501..4701914 (+) 414 WP_180807567.1 8-oxo-dGTP diphosphatase MutT -
  U8518_RS21580 (U8518_21580) yacG 4701975..4702169 (-) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  U8518_RS21585 (U8518_21585) zapD 4702179..4702901 (-) 723 WP_180807569.1 cell division protein ZapD -
  U8518_RS21590 (U8518_21590) coaE 4702939..4703553 (-) 615 WP_101149980.1 dephospho-CoA kinase -
  U8518_RS21595 (U8518_21595) pilD 4703572..4704444 (-) 873 WP_101149981.1 prepilin peptidase Machinery gene
  U8518_RS21600 (U8518_21600) pilC 4704467..4705708 (-) 1242 WP_101149982.1 type II secretion system F family protein Machinery gene
  U8518_RS21605 (U8518_21605) pilB 4705824..4707539 (-) 1716 WP_180807586.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  U8518_RS21610 (U8518_21610) - 4707530..4707970 (-) 441 WP_043160631.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  U8518_RS21615 (U8518_21615) nadC 4708293..4709150 (-) 858 WP_101149983.1 carboxylating nicotinate-nucleotide diphosphorylase -
  U8518_RS21620 (U8518_21620) - 4709156..4709626 (-) 471 WP_101149984.1 TIGR02281 family clan AA aspartic protease -
  U8518_RS21625 (U8518_21625) ampD 4709773..4710345 (+) 573 WP_101149985.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  U8518_RS21630 (U8518_21630) pdhR 4710621..4711385 (+) 765 WP_011707564.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62880.40 Da        Isoelectric Point: 5.4342

>NTDB_id=916735 U8518_RS21605 WP_180807586.1 4705824..4707539(-) (pilB) [Aeromonas hydrophila strain MaAh001]
MPIMTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIP
QKYLNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTTAIGKLMENDQDALGMEDIDE
SEISELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRF
SARLKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALS
KPQGMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGE
IRDLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLEL
GFTQQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVT
SLAEINRVTTN

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=916735 U8518_RS21605 WP_180807586.1 4705824..4707539(-) (pilB) [Aeromonas hydrophila strain MaAh001]
CTGCCCATAATGACATCTAGCCCCAATAGCGGCCTGGCATTAAGCTTGGCCGCCTCTTCCCTGCTCAGTGAGTCCGACTC
ACAACGCTATCTGAGCCAAGCCAAGGCACAGCGCAAACCTTTTGTGACTTTTCTGATCGAAAACGAAATCCTCGACAGCA
AGGCACTGGCTGACTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCG
CAGAAGTACCTCAATCAGAAGCTGATTGAAAAACATCACGTCCTACCCATCTACACCCAAGGGCACACCCTCTATATCGC
CATGTCAGACCCGACCAATGTGTCTGCACTGGAGGATTTTGGCTTCAGCTTCGGGTTGCATACCGAGGCGCTGCTGGTCG
AGGAGAACAAACTCACCACCGCCATCGGCAAGCTGATGGAGAACGATCAGGATGCCCTCGGCATGGAGGACATCGACGAA
TCCGAGATTTCCGAGCTGGAAGTGTCGGATGAGGGATCGCGGCTCGACGAGAGCGTCAATACGGCCGACGACGACGCGCC
CATCGTCAAATACATCAACAAAATCATGATGGATGCTATCAAGCGCGGAGCCTCCGACCTCCACTTCGAACCCTATGAGA
CCAAATATCGCATCCGCTTTCGGATAGACGGGATCTTGCATGAAATAGCGACCCCACCGGTCAACCTGGCCAACCGCTTC
TCCGCCCGCCTCAAGGTGATGGCACGGCTCGACATCGCCGAGCGGCGACTGCCCCAGGATGGTCGCATCAAACTGAAGCT
GTCGCGCAACAAGTCGATGGATATGCGGGTCAATACCCTGCCCACCATGTGGGGCGAAAAGATCGTTATCCGTTTGCTCG
ACTCCTCCGCGGCACGCCTCAATATCGAGCAGCTCGGTTTTGACGATCGCCAGAAGGCGCAATACCTGCGTGCCCTGTCG
AAGCCTCAGGGCATGATTCTGGTGACCGGCCCTACCGGTTCGGGCAAGACGGTTTCACTCTATACCGGTCTCAATATTCT
CAATACCACCGAGGTCAATATCTCCACCGCCGAAGATCCGGTGGAGATCAACCTGCCCGGCGTCAATCAGGTGCAAGTCA
ATCCCAAGGCAGGACTGACCTTCGCGAGCGCCCTGCGCTCTTTCTTGCGCCAAGACCCGGATGTGGTCATGGTGGGTGAA
ATCCGGGATCTGGAGACCGCTGAAATTGCCATCAAGGCCGCCCAGACCGGCCACCTGGTGCTATCCACCCTGCACACCAA
CTCGGCGGCAGAAACGCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAATATCGCCTCCTCGGTCACCCTGATCA
TGGCTCAGCGGCTGGCGCGTAAATTGTGCGATCACTGCAAAGCTCCCGAAGTGGTGCCAGAGGCTGAGTTGCTTGAGCTG
GGTTTTACCCAGCAGCAGCTGGCCGCAGGCTTGCGGCTGTTCAAACCGGTTGGCTGCAAGGAGTGTTCGGGTGGCTACAA
GGGCCGGGTCGGCATCTACGAAATCATGCTAATGTCTGAAAACATCGCAAAATTGATCATGCAGGGGGCCAACTCTCTGC
AAATTGCCGCCATCGCCCAGAAGGAGGGCATGCGTACCTTGCGTACCTCCGGCCTTGAGAAGGCCCGCCTAGGGGTGACC
AGTCTGGCGGAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.401

100

0.557
  pilB Acinetobacter baylyi ADP1

55.323

100

0.555
  pilB Legionella pneumophila strain ERS1305867

54.064

99.124

0.536
  pilB Vibrio cholerae strain A1552

53.982

98.949

0.534
  pilB Vibrio campbellii strain DS40M4

51.943

99.124

0.515
  pilB Vibrio parahaemolyticus RIMD 2210633

51.423

98.424

0.506
  pilF Neisseria gonorrhoeae MS11

46.454

98.774

0.459
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

96.848

0.377
  pilF Thermus thermophilus HB27

40.152

92.469

0.371