Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   U3649_RS06900 Genome accession   NZ_CP141262
Coordinates   1648762..1649181 (-) Length   139 a.a.
NCBI ID   WP_407354123.1    Uniprot ID   -
Organism   Luteimonas sp. R10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1637481..1649376 1648762..1649181 within 0


Gene organization within MGE regions


Location: 1637481..1649376
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U3649_RS06850 (U3649_06815) - 1637481..1638332 (+) 852 WP_407354113.1 glycosyltransferase family 2 protein -
  U3649_RS06855 (U3649_06820) - 1638397..1640256 (+) 1860 WP_407354114.1 glycosyltransferase -
  U3649_RS06860 (U3649_06825) - 1640199..1641143 (-) 945 WP_407354115.1 glycosyltransferase family 2 protein -
  U3649_RS06865 (U3649_06830) - 1641155..1642036 (-) 882 WP_407354116.1 glycosyltransferase -
  U3649_RS06870 (U3649_06835) - 1642046..1643512 (-) 1467 WP_407354117.1 MOP flippase family protein -
  U3649_RS06875 (U3649_06840) - 1643509..1644186 (-) 678 WP_407354118.1 methyltransferase domain-containing protein -
  U3649_RS06880 (U3649_06845) - 1644187..1644855 (-) 669 WP_407354119.1 methyltransferase domain-containing protein -
  U3649_RS06885 (U3649_06850) - 1644852..1646006 (-) 1155 WP_407354120.1 glycosyltransferase -
  U3649_RS06890 (U3649_06855) - 1646252..1648135 (-) 1884 WP_407354121.1 tetratricopeptide repeat protein -
  U3649_RS06895 (U3649_06860) - 1648229..1648636 (-) 408 WP_407354122.1 pilin -
  U3649_RS06900 (U3649_06865) pilA/pilAI 1648762..1649181 (-) 420 WP_407354123.1 pilin Machinery gene

Sequence

Protein


Download         Length: 139 a.a.        Molecular weight: 14584.48 Da        Isoelectric Point: 4.5971

>NTDB_id=916501 U3649_RS06900 WP_407354123.1 1648762..1649181(-) (pilA/pilAI) [Luteimonas sp. R10]
MKKQQGFTLIELMIVVAIIAILAAIALPAYQNYVAKSQATAGLADIRGGVTAYEEGIQSGRSGTPDLEAIGLSDSTPRCA
ISIPDPSWTEARGQSIVCTLKGNPNVVGETITLTRNSSGQWPCSVSDDLHENHTPGGCE

Nucleotide


Download         Length: 420 bp        

>NTDB_id=916501 U3649_RS06900 WP_407354123.1 1648762..1649181(-) (pilA/pilAI) [Luteimonas sp. R10]
ATGAAGAAGCAGCAAGGCTTTACCCTGATCGAACTGATGATCGTCGTCGCGATCATCGCCATCCTGGCGGCCATCGCGCT
GCCGGCCTACCAGAACTACGTTGCCAAGTCGCAGGCCACTGCTGGTCTGGCCGATATCCGTGGCGGCGTGACTGCATACG
AAGAAGGCATCCAGTCCGGACGCTCGGGGACTCCCGACCTGGAAGCCATTGGCCTGTCGGACTCCACACCTCGCTGTGCC
ATCTCGATTCCCGATCCGTCGTGGACGGAAGCAAGAGGCCAGAGCATTGTGTGCACGCTGAAGGGTAACCCGAACGTTGT
CGGCGAAACGATCACTCTGACCCGCAATAGCTCCGGTCAATGGCCCTGCAGCGTCTCTGACGATCTCCATGAAAATCATA
CGCCGGGCGGCTGCGAGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-138)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

47.183

100

0.482
  pilA Pseudomonas aeruginosa PAK

43.046

100

0.468
  pilA/pilAII Pseudomonas stutzeri DSM 10701

45.39

100

0.46
  pilA Acinetobacter baumannii strain A118

44.604

100

0.446
  pilA Vibrio cholerae strain A1552

39.583

100

0.41
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.583

100

0.41
  pilA Vibrio cholerae C6706

39.583

100

0.41
  comP Acinetobacter baylyi ADP1

35.294

100

0.388