Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OCV30_RS12695 Genome accession   NZ_AP025460
Coordinates   2862529..2864214 (+) Length   561 a.a.
NCBI ID   WP_065678065.1    Uniprot ID   -
Organism   Vibrio atlanticus strain CECT 7223     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2857529..2869214
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV30_RS12675 pdhR 2859057..2859818 (-) 762 WP_009847596.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  OCV30_RS12680 ampD 2860301..2860901 (-) 601 Protein_2453 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  OCV30_RS12685 nadC 2860985..2861875 (+) 891 WP_017103843.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OCV30_RS12690 pilA 2862130..2862528 (+) 399 WP_065678066.1 pilin Machinery gene
  OCV30_RS12695 pilB 2862529..2864214 (+) 1686 WP_065678065.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OCV30_RS12700 pilC 2864230..2865456 (+) 1227 WP_065678064.1 type II secretion system F family protein Machinery gene
  OCV30_RS12705 pilD 2865525..2866394 (+) 870 WP_065678063.1 prepilin peptidase Machinery gene
  OCV30_RS12710 coaE 2866397..2867011 (+) 615 WP_065678062.1 dephospho-CoA kinase -
  OCV30_RS12715 zapD 2867038..2867778 (+) 741 WP_065678061.1 cell division protein ZapD -
  OCV30_RS12720 yacG 2868022..2868219 (+) 198 WP_065678060.1 DNA gyrase inhibitor YacG -
  OCV30_RS12725 rplS 2868547..2868900 (-) 354 WP_009847607.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62055.76 Da        Isoelectric Point: 5.1205

>NTDB_id=91650 OCV30_RS12695 WP_065678065.1 2862529..2864214(+) (pilB) [Vibrio atlanticus strain CECT 7223]
MLTNLPTVLRQANLLSLTQKQAVTEQVQASGGSTPEALLTLGLFTGESLAHNIKAIFGLPLVQLGNTDYEALCEQLGLRE
LITKYCAIPVSISNSTLTLASADPTDLQAEDDFRFATGLQIELVVANYSELEGAIRKLYGRSISGQDSKRKEITQDELAN
LVEVSDDEMSSIEDLSQDDSPVSRFINQILVDAVRKGASDIHFEPYEENYRVRLRCDGILVEVQQPASHLSRRLSARLKI
LAKLDIAERRLPQDGRIKLRLNDELAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSEDQKALYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRMLNTSERNISTAEDPVEINLCGINQVQVTPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAIKASQTGHLVLSTLHTNSASETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCNHCKTADNSPDIFLRHSIPNSQN
IYKATPQGCNECNQGYSGRVGIYEVMPFTDKLKNSLINKPNALEIEDLARREGMRTLQESGIDKLLEGTTSYQELQRVLY
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=91650 OCV30_RS12695 WP_065678065.1 2862529..2864214(+) (pilB) [Vibrio atlanticus strain CECT 7223]
GTGCTAACTAACCTCCCAACCGTTCTCCGTCAGGCTAACTTGCTTAGCCTGACTCAAAAACAAGCTGTTACAGAGCAGGT
TCAAGCTTCTGGTGGGTCTACGCCTGAAGCTTTGCTCACTTTGGGCTTATTCACCGGCGAGAGTCTCGCGCATAACATTA
AGGCCATTTTCGGCTTGCCGTTGGTACAACTTGGCAATACGGACTATGAAGCTTTATGCGAACAGTTAGGTCTTCGTGAA
CTGATCACCAAGTACTGTGCTATCCCAGTTTCCATATCTAATTCTACTCTCACACTCGCCTCTGCCGATCCAACCGACTT
GCAAGCTGAAGACGATTTTAGATTCGCGACTGGATTGCAGATAGAACTCGTTGTCGCTAACTATTCAGAATTAGAAGGCG
CCATACGAAAGCTGTATGGTCGCTCTATTTCCGGGCAAGACTCCAAGCGCAAAGAGATCACCCAAGATGAGCTCGCCAAT
CTTGTTGAAGTCTCTGATGATGAAATGAGTTCAATTGAAGATCTCAGTCAAGACGACTCCCCTGTCAGTCGATTTATCAA
TCAGATACTGGTTGATGCGGTGCGTAAAGGCGCATCAGACATCCACTTCGAACCTTATGAAGAAAACTACCGAGTGCGTC
TGCGTTGCGATGGCATTCTTGTTGAGGTCCAACAACCCGCGTCTCATTTAAGCCGTCGGTTATCCGCTCGTTTAAAGATT
CTCGCCAAACTTGATATCGCTGAACGTCGCTTACCTCAAGACGGCCGTATTAAGCTGCGTTTGAACGACGAGCTGGCGAT
CGATATGCGTGTGTCGACTCTGCCGACATTGTGGGGAGAAAAGATCGTACTGCGTCTTCTAGACAGCAGCGCAGCCAATT
TAGATATCGATAAATTAGGTTACAGCGAGGATCAAAAAGCGCTCTATCTCAATGCCCTAAAACGCCCCCAAGGGATGATC
TTAATGACTGGGCCCACAGGCAGTGGCAAAACGGTATCTCTTTACACTGGACTTCGCATGCTTAACACCTCTGAGCGTAA
TATCTCAACAGCTGAAGATCCGGTGGAGATCAACCTGTGTGGTATCAATCAGGTACAGGTGACGCCAAAGATCGGTTTTG
GGTTTGCCGAAGCACTACGATCGTTCTTGCGACAAGACCCTGATGTAGTGATGGTCGGTGAGATCCGTGATTTAGAAACC
GCAGAGATCGCGATCAAAGCATCGCAAACGGGTCACTTAGTCCTCTCGACACTCCATACTAACTCAGCTTCGGAAACCGT
GACTCGACTCGCCCATATGGGGATTGAACCATTTAACCTCGCCTCGTCATTAAGCCTGATTATCGCGCAGCGACTGGCAA
GGCGACTGTGCAACCACTGTAAAACAGCTGACAACTCGCCGGACATATTTCTACGTCACTCTATTCCTAACAGCCAAAAC
ATCTACAAAGCCACCCCACAAGGATGCAATGAGTGTAATCAGGGTTACTCCGGTCGAGTGGGTATCTACGAAGTAATGCC
GTTCACCGACAAACTAAAAAATAGTCTGATCAATAAACCTAACGCGTTAGAGATTGAAGATCTCGCGCGTAGAGAAGGAA
TGAGAACACTGCAAGAATCCGGTATCGATAAGTTGCTTGAAGGCACCACCAGCTATCAAGAACTGCAACGTGTTCTGTAT
CTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

73.179

100

0.734
  pilB Vibrio parahaemolyticus RIMD 2210633

70.641

100

0.708
  pilB Vibrio campbellii strain DS40M4

70.107

100

0.702
  pilB Acinetobacter baumannii D1279779

50.581

91.979

0.465
  pilB Legionella pneumophila strain ERS1305867

49.427

93.405

0.462
  pilB Acinetobacter baylyi ADP1

48.837

91.979

0.449
  pilF Neisseria gonorrhoeae MS11

43.794

100

0.44
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.139

97.683

0.373
  pilF Thermus thermophilus HB27

37.41

99.109

0.371

Multiple sequence alignment