Detailed information NTG0091652

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: OCV30_RS12705
• Genome accession: NZ_AP025460
• Coordinates: 2865525..2866394 (+)
• Length: 289 a.a.
• NCBI ID: WP_065678063.1
• Uniprot ID: A0A1C3IGU5
• Organism: Vibrio atlanticus strain CECT 7223
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2860525..2871394

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OCV30_RS12685	nadC	2860985..2861875 (+)	891	WP_017103843.1	carboxylating nicotinate-nucleotide diphosphorylase	-
OCV30_RS12690	pilA	2862130..2862528 (+)	399	WP_065678066.1	pilin	Machinery gene
OCV30_RS12695	pilB	2862529..2864214 (+)	1686	WP_065678065.1	type IV-A pilus assembly ATPase PilB	Machinery gene
OCV30_RS12700	pilC	2864230..2865456 (+)	1227	WP_065678064.1	type II secretion system F family protein	Machinery gene
OCV30_RS12705	pilD	2865525..2866394 (+)	870	WP_065678063.1	prepilin peptidase	Machinery gene
OCV30_RS12710	coaE	2866397..2867011 (+)	615	WP_065678062.1	dephospho-CoA kinase	-
OCV30_RS12715	zapD	2867038..2867778 (+)	741	WP_065678061.1	cell division protein ZapD	-
OCV30_RS12720	yacG	2868022..2868219 (+)	198	WP_065678060.1	DNA gyrase inhibitor YacG	-
OCV30_RS12725	rplS	2868547..2868900 (-)	354	WP_009847607.1	50S ribosomal protein L19	-
OCV30_RS12730	trmD	2868960..2869700 (-)	741	WP_009847608.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
OCV30_RS12735	rimM	2869727..2870281 (-)	555	WP_065678059.1	ribosome maturation factor RimM	-
OCV30_RS12740	rpsP	2870308..2870556 (-)	249	WP_004735508.1	30S ribosomal protein S16	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32482.71 Da
• Isoelectric Point: 7.9546

>NTDB_id=91652 OCV30_RS12705 WP_065678063.1 2865525..2866394(+) (pilD) [Vibrio atlanticus strain CECT 7223]
MEVFHYYPWLFPVLAFIFSLLIGSFLNVVIHRLPIMMEREWQQECSEYFSQYKIPAPEGKFNLSIPRSACPKCKTQLRIV
DNVPVLSWLFLKGKCHSCANPISARYPLVELLTAILCTVVASHFGFSYYAIALIFFTFALIAATFIDLDTMLLPDQITLP
LVWSGIALALFNISPVSLQNSVVGAMAGYLALWSVYWLFKLLTGKEGMGYGDFKLLAALGAWLGWQHLPMIILLSSLVGL
VFGLIQLRLKQQGIDKAFPFGPYLAIAGWVSLMWGNDIMGWYFTSVLGI

Nucleotide

• Length: 870 bp

>NTDB_id=91652 OCV30_RS12705 WP_065678063.1 2865525..2866394(+) (pilD) [Vibrio atlanticus strain CECT 7223]
ATGGAAGTATTTCACTACTATCCTTGGCTATTCCCCGTATTAGCTTTCATTTTTAGCCTTCTTATTGGCAGTTTCCTCAA
CGTAGTCATACACCGTTTACCGATTATGATGGAACGAGAGTGGCAACAAGAGTGCTCGGAGTATTTCTCTCAATATAAAA
TTCCAGCGCCAGAGGGGAAGTTCAATCTCAGTATCCCTCGTTCGGCTTGCCCGAAATGTAAAACCCAATTAAGAATCGTC
GACAATGTTCCAGTATTAAGCTGGTTGTTCTTAAAAGGGAAGTGTCATAGCTGTGCTAACCCAATCAGTGCTCGCTATCC
ATTGGTCGAACTGCTGACTGCAATACTATGCACCGTAGTGGCTAGCCACTTTGGTTTCAGTTACTACGCCATTGCTTTGA
TTTTTTTCACCTTTGCATTGATTGCCGCAACCTTTATCGACCTAGATACCATGCTGTTACCTGATCAAATCACCTTACCT
TTGGTTTGGTCTGGTATCGCTTTAGCACTGTTTAACATCAGCCCTGTCTCGCTTCAAAATTCCGTAGTTGGCGCAATGGC
TGGCTATCTAGCTTTGTGGTCTGTTTATTGGTTGTTCAAGCTACTAACAGGCAAAGAAGGCATGGGATATGGAGACTTCA
AACTCCTAGCCGCATTAGGTGCATGGCTTGGCTGGCAACACCTACCAATGATCATTCTTTTGTCCTCGCTGGTTGGTTTG
GTTTTCGGCTTGATTCAACTTCGCTTGAAACAGCAAGGCATAGATAAAGCCTTCCCATTTGGACCTTACCTGGCGATTGC
GGGTTGGGTAAGTTTGATGTGGGGCAATGACATCATGGGTTGGTATTTCACTTCTGTACTAGGGATTTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

• Type IV leader peptidase family (Peptidase_A24) (135-244)

3D structure

• AlphaFold DB: A0A1C3IGU5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	74.048	100	0.74
pilD	Vibrio cholerae strain A1552	68.056	99.654	0.678
pilD	Acinetobacter nosocomialis M2	51.311	92.388	0.474
pilD	Acinetobacter baumannii D1279779	50.936	92.388	0.471
pilD	Neisseria gonorrhoeae MS11	46.014	95.502	0.439