Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   U8638_RS22675 Genome accession   NZ_CP141060
Coordinates   5133492..5134664 (+) Length   390 a.a.
NCBI ID   WP_084104044.1    Uniprot ID   A0A239SW42
Organism   Pandoraea sputorum strain EFB03792     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5128492..5139664
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U8638_RS22640 cgtA 5128597..5129727 (-) 1131 WP_039393308.1 Obg family GTPase CgtA -
  U8638_RS22645 rpmA 5129877..5130137 (-) 261 WP_039393311.1 50S ribosomal protein L27 -
  U8638_RS22650 rplU 5130166..5130477 (-) 312 WP_039393314.1 50S ribosomal protein L21 -
  U8638_RS22655 - 5130805..5131734 (+) 930 WP_224786948.1 polyprenyl synthetase family protein -
  U8638_RS22665 - 5132021..5132146 (-) 126 WP_257125707.1 hypothetical protein -
  U8638_RS22670 - 5132145..5133350 (+) 1206 WP_257125708.1 HlyC/CorC family transporter -
  U8638_RS22675 pilF 5133492..5134664 (+) 1173 WP_084104044.1 GspE/PulE family protein Machinery gene
  U8638_RS22680 - 5134803..5136029 (+) 1227 WP_322880903.1 type II secretion system F family protein -
  U8638_RS22685 - 5136026..5136886 (+) 861 WP_052252325.1 prepilin peptidase -
  U8638_RS22690 coaE 5136883..5137497 (+) 615 WP_322880904.1 dephospho-CoA kinase -
  U8638_RS22695 zapD 5137673..5138428 (+) 756 WP_039393327.1 cell division protein ZapD -
  U8638_RS22700 yacG 5138433..5138627 (+) 195 WP_039393329.1 DNA gyrase inhibitor YacG -
  U8638_RS22705 - 5138764..5139198 (-) 435 WP_150777649.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 390 a.a.        Molecular weight: 41994.23 Da        Isoelectric Point: 7.4150

>NTDB_id=915795 U8638_RS22675 WP_084104044.1 5133492..5134664(+) (pilF) [Pandoraea sputorum strain EFB03792]
MLRPTIASDDALPSTGTPASTTPLQRLEQILSEAIRAGASDIHFEPMAQRFRVRLRVDGRLQEHGDVPLTARDMLVSRLK
VLANLDIAQKRLPQDGRMVWREAGEPVECRVSALPTLHGEKLVVRLLDGAKVPLSLEGLGYSPKQYLALTQAIARPHGLI
LLTGPTGSGKTVSLYSCLRRLNDASRNIVTIEDPVEIRLAGITQVNLNERAGLDFATALRAFLRQDPDVLVVGEIRDAQT
AEIAIQAAQTGHLVFATVHANDAPSTLARLFDLGVAPFNLSSTLLLVSAQRLLRRRCPAGCEPSRTTTTETGLADTCMRC
HGTGFAGRIGAFQVMPISATQAINIAQARSPHELAAQACREGVMTLREAGLWHVDAGAVAQEDVDATLPA

Nucleotide


Download         Length: 1173 bp        

>NTDB_id=915795 U8638_RS22675 WP_084104044.1 5133492..5134664(+) (pilF) [Pandoraea sputorum strain EFB03792]
ATGCTGCGTCCAACCATCGCCTCCGACGACGCCCTGCCGTCCACCGGCACCCCGGCGTCCACCACACCTCTGCAACGCCT
TGAGCAAATACTGAGCGAAGCGATTCGTGCGGGCGCTTCCGATATCCATTTCGAACCGATGGCGCAGCGCTTTCGGGTCC
GTCTGCGTGTCGACGGTCGCTTGCAGGAGCATGGCGACGTTCCGCTGACAGCGCGCGACATGCTGGTCTCGCGTCTGAAG
GTGCTTGCCAATCTCGACATCGCACAAAAGCGCTTGCCTCAGGACGGACGCATGGTGTGGCGCGAAGCTGGTGAACCGGT
CGAGTGTCGCGTGAGTGCGCTTCCCACCCTGCACGGCGAGAAACTCGTGGTGCGCCTGCTCGATGGCGCGAAAGTCCCTC
TCTCGCTTGAAGGTCTCGGCTATTCGCCGAAGCAATACCTCGCACTCACGCAAGCGATTGCCCGGCCACATGGCCTGATC
TTGCTGACGGGCCCGACGGGCAGTGGCAAGACCGTTTCGCTCTATAGTTGCCTGCGACGTCTGAACGACGCGTCGCGCAA
CATCGTCACCATCGAGGACCCGGTGGAAATTCGCTTGGCCGGTATCACGCAGGTCAATCTCAACGAACGCGCCGGCCTCG
ACTTTGCAACGGCTTTACGTGCGTTTCTCCGTCAGGATCCAGATGTCCTCGTTGTGGGCGAAATCCGCGATGCGCAGACC
GCCGAAATCGCCATACAGGCCGCGCAGACCGGTCACCTTGTGTTCGCCACCGTTCACGCCAACGACGCGCCGAGCACGCT
CGCGCGGCTCTTCGATCTCGGTGTCGCCCCGTTCAATCTGTCGTCGACATTACTGCTGGTCAGCGCACAGCGTCTGCTTA
GGCGACGATGCCCGGCCGGGTGCGAGCCGTCCCGCACGACGACGACTGAAACAGGCCTCGCTGACACCTGCATGCGGTGT
CACGGCACCGGTTTTGCCGGACGCATCGGCGCGTTTCAGGTGATGCCAATCAGCGCCACGCAGGCTATCAACATTGCACA
GGCGCGCAGTCCGCACGAGCTGGCGGCGCAGGCATGCCGCGAAGGCGTCATGACGCTGCGAGAAGCCGGGTTATGGCATG
TCGATGCAGGGGCTGTCGCTCAGGAGGATGTCGATGCGACGCTGCCCGCTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(29-295)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A239SW42

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

48.462

100

0.485
  pilB Vibrio cholerae strain A1552

47.409

98.974

0.469
  pilB Vibrio parahaemolyticus RIMD 2210633

46.194

97.692

0.451
  pilB Acinetobacter baumannii D1279779

43.75

100

0.449
  pilB Vibrio campbellii strain DS40M4

47.297

94.872

0.449
  pilB Acinetobacter baylyi ADP1

45.789

97.436

0.446
  pilB Legionella pneumophila strain ERS1305867

42.786

100

0.441
  pilF Thermus thermophilus HB27

41.582

100

0.418
  pilB/pilB1 Synechocystis sp. PCC 6803

39.853

100

0.418
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.379

96.667

0.4
  pilB Haemophilus influenzae 86-028NP

42.149

93.077

0.392
  pilB Haemophilus influenzae Rd KW20

46.452

79.487

0.369
  pilB Glaesserella parasuis strain SC1401

38.919

94.872

0.369