Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   U6038_RS01640 Genome accession   NZ_CP140715
Coordinates   338036..339748 (-) Length   570 a.a.
NCBI ID   WP_333654880.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain FAHZZU3110hy     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 333036..344748
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U6038_RS01610 (U6038_01610) - 333131..333454 (+) 324 WP_004692718.1 pyrimidine/purine nucleoside phosphorylase -
  U6038_RS01615 (U6038_01615) rlmB 333598..334347 (+) 750 WP_004692717.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  U6038_RS01620 (U6038_01620) - 334380..335297 (+) 918 WP_205667275.1 DMT family transporter -
  U6038_RS01625 (U6038_01625) coaE 335311..335910 (-) 600 WP_333654876.1 dephospho-CoA kinase -
  U6038_RS01630 (U6038_01630) pilD 335919..336779 (-) 861 WP_058868793.1 prepilin peptidase Machinery gene
  U6038_RS01635 (U6038_01635) pilC 336779..338005 (-) 1227 WP_010325767.1 type II secretion system F family protein Machinery gene
  U6038_RS01640 (U6038_01640) pilB 338036..339748 (-) 1713 WP_333654880.1 type IV-A pilus assembly ATPase PilB Machinery gene
  U6038_RS01645 (U6038_01645) tpiA 340028..340819 (+) 792 WP_333654883.1 triose-phosphate isomerase -
  U6038_RS01650 (U6038_01650) secG 340833..341162 (+) 330 WP_004692710.1 preprotein translocase subunit SecG -
  U6038_RS01680 (U6038_01680) rimP 342116..342640 (+) 525 WP_004692708.1 ribosome maturation factor RimP -
  U6038_RS01685 (U6038_01685) nusA 342680..344164 (+) 1485 WP_004692706.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63682.04 Da        Isoelectric Point: 4.9493

>NTDB_id=915110 U6038_RS01640 WP_333654880.1 338036..339748(-) (pilB) [Acinetobacter johnsonii strain FAHZZU3110hy]
MSVSNGLPKFTGFIRRLVEDGRMTAENMQQAMLSAKKAQQDIVPYLIENYKLSPQMIAETISLEFGEPVFDLSVYDSVQI
LRDLVEDKLITKHRMLPIYRRGNILYIATSNPTNMDAMDAIRFNSKMNIEAIIVEHPQLEKLIEQNFAQADSFEFSDEDI
DLDLEQDHQNSNDEEDDSPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRHIASPPLQLATRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKDLFMQALDKP
QGMLLITGPTGSGKTVSLYTGLNILNKEDTNISTAEDPVEINLEGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKAAVDIPEQSLLEMGF
TSTDIKDPDFKVYQPVGCTECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAEASAKLGFNNLRRSGLLKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=915110 U6038_RS01640 WP_333654880.1 338036..339748(-) (pilB) [Acinetobacter johnsonii strain FAHZZU3110hy]
ATGTCAGTATCAAATGGTTTGCCTAAGTTCACAGGCTTCATTCGACGCTTAGTCGAAGACGGACGCATGACTGCTGAAAA
TATGCAGCAGGCAATGCTCTCTGCAAAAAAGGCTCAGCAAGACATCGTTCCTTATCTAATTGAAAATTATAAACTCTCCC
CACAAATGATCGCTGAGACGATTTCTTTGGAGTTTGGCGAGCCTGTTTTTGACTTAAGTGTCTATGATTCGGTACAAATT
CTACGAGATCTGGTCGAAGACAAACTCATCACCAAGCATCGTATGTTGCCGATTTACCGTCGTGGCAATATCCTCTATAT
CGCCACCAGCAATCCAACCAACATGGATGCAATGGACGCGATTCGTTTCAATTCTAAAATGAACATTGAAGCGATTATTG
TTGAACATCCTCAGCTTGAAAAACTAATCGAACAAAATTTCGCGCAAGCCGATAGCTTTGAATTTTCTGATGAAGATATT
GATTTAGACTTAGAGCAAGACCATCAGAACTCAAATGACGAAGAAGATGACTCACCTCAAGGGGATGAAGCGCCGATCGT
CAAATACATCAATAAATTACTGATCGATGCGATTCGTATGGGCGCATCAGACTTGCACTTTGAACCTTATGAAAAATCTT
ACCGTGTCCGTTATCGTGTAGATGGGGTTCTGCGTCACATTGCCAGCCCACCACTACAACTGGCGACTCGTCTTGCTTCA
CGTTTAAAAGTTATGTCACAAATGGACATCTCTGAAAAGCGCGTCCCGCAAGATGGTCGTATTAAATTAAAGCTGTCGAA
AAGCAAAGCCATCGACTTCCGTGTCAACTCACTTCCAACCTTATTTGGCGAAAAGCTGGTTCTACGTATTCTCGATCCAT
CCAGTGCTATGCTGGGGATTGATGCTTTAGGTTATGAACCAGATCAAAAAGACCTATTTATGCAGGCTTTAGACAAACCG
CAAGGCATGTTGTTAATTACTGGCCCGACAGGTTCAGGTAAAACGGTATCGCTATATACAGGTTTGAATATTCTAAATAA
AGAAGACACTAATATCTCAACTGCAGAAGACCCCGTTGAGATTAACTTAGAAGGTATTAATCAGGTCAATGTGAACAATA
AAGTCGGCCTCACCTTCTCTGCTGCGCTGAAGTCCTTCTTACGTCAGGATCCTGATATTGTAATGGTGGGTGAGATTCGT
GACCTTGAAACGGCTGAAATCGCGATTAAAGCCGCACAGACAGGCCACATGGTCATGTCGACACTCCACACCAATAGTGC
ACCTGAAACTCTGACACGCTTACGCAATATGGGGGTTCCATCTTTTAACATTGCGACATCGGTCAATTTGGTAATTGCAC
AGCGTTTGGCACGCCGTTTATGTAGCCAATGCAAAGCCGCTGTCGATATTCCTGAACAAAGCTTGCTCGAAATGGGCTTT
ACTTCAACAGATATCAAAGATCCAGACTTCAAAGTTTATCAACCGGTGGGATGTACTGAGTGCCGTGAAGGCTATAAAGG
ACGTGTTGGTATTTATGAAGTCATGAAAGTCACACCAGAAATTTCAAAAATTATTATGGAAGATGGCAATGCCTTACAGA
TTGCGGAAGCATCTGCAAAATTAGGATTTAACAATTTACGTCGTTCAGGTTTACTCAAGGTTATGCAGGGGGTCACTTCC
TTACAAGAAGTGAATCGTGTAACCAGCGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.228

100

0.812
  pilB Acinetobacter baylyi ADP1

76.667

100

0.767
  pilB Legionella pneumophila strain ERS1305867

54.12

93.684

0.507
  pilF Neisseria gonorrhoeae MS11

49.822

98.596

0.491
  pilB Vibrio cholerae strain A1552

48.677

99.474

0.484
  pilB Vibrio parahaemolyticus RIMD 2210633

50.677

90.702

0.46
  pilB Vibrio campbellii strain DS40M4

50

90.877

0.454
  pilB/pilB1 Synechocystis sp. PCC 6803

34.41

100

0.374
  pilF Thermus thermophilus HB27

36.427

100

0.365