Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   TQH59_RS13220 Genome accession   NZ_CP140708
Coordinates   2661048..2662169 (+) Length   373 a.a.
NCBI ID   WP_004984301.1    Uniprot ID   N9BCS7
Organism   Acinetobacter johnsonii strain FAHZZU3567hy     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2656048..2667169
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TQH59_RS13205 (TQH59_13205) - 2658250..2658984 (-) 735 WP_333640499.1 hypothetical protein -
  TQH59_RS13210 (TQH59_13210) - 2659175..2659861 (-) 687 WP_333640500.1 YggS family pyridoxal phosphate-dependent enzyme -
  TQH59_RS13215 (TQH59_13215) pilT 2659984..2661021 (+) 1038 WP_004694862.1 type IV pilus twitching motility protein PilT Machinery gene
  TQH59_RS13220 (TQH59_13220) pilU 2661048..2662169 (+) 1122 WP_004984301.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  TQH59_RS13225 (TQH59_13225) fur 2662230..2662667 (-) 438 WP_004694866.1 ferric iron uptake transcriptional regulator -
  TQH59_RS13230 (TQH59_13230) - 2662780..2663178 (+) 399 WP_004694868.1 outer membrane protein assembly factor BamE -
  TQH59_RS13235 (TQH59_13235) - 2663225..2663548 (-) 324 WP_333640501.1 RnfH family protein -
  TQH59_RS13240 (TQH59_13240) - 2663548..2664630 (-) 1083 WP_333640502.1 hypothetical protein -
  TQH59_RS13245 (TQH59_13245) - 2664773..2665228 (-) 456 WP_005400160.1 bacteriohemerythrin -
  TQH59_RS13250 (TQH59_13250) - 2665354..2665746 (-) 393 Protein_2568 transposase family protein -
  TQH59_RS13255 (TQH59_13255) - 2665784..2666188 (-) 405 WP_004691434.1 transposase family protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41849.12 Da        Isoelectric Point: 6.6662

>NTDB_id=915085 TQH59_RS13220 WP_004984301.1 2661048..2662169(+) (pilU) [Acinetobacter johnsonii strain FAHZZU3567hy]
MDFNDLLNYMIQQKASDLFITADVEPSMKINGQIVPVAKGKLTGDVAGQLLNSIMSDKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETQIPTMDELKLPPVLKELAMTKRGIIIFVGATGTGKSTSLASIIGYRNQNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFEADRHSQLFMDLSLNLKAMVAQQLIPTPDGNSRRAAIEILINSPLISDLIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAKQITYKDALKHADSPNDLRLQIKLSEEGGDRLLHASANITFDGQA

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=915085 TQH59_RS13220 WP_004984301.1 2661048..2662169(+) (pilU) [Acinetobacter johnsonii strain FAHZZU3567hy]
ATGGACTTTAACGACTTACTCAATTATATGATTCAACAAAAAGCGTCGGATTTATTTATCACCGCTGATGTTGAACCTTC
CATGAAAATTAACGGTCAAATTGTGCCTGTGGCTAAGGGTAAGCTGACGGGTGATGTCGCGGGGCAACTGCTCAACTCGA
TTATGAGTGACAAGCAGCGTAAAGAATTTGCGGACACACGTGAGTGCAACTTCGCGATTATGAATCGGGATAAAACTGCA
CGTTTTCGTGTCAGTGCCTTTCAACAACGTGATATGCCAGGTATGGTTTTGCGTCGGATTGAGACGCAAATTCCAACCAT
GGACGAGTTAAAACTACCGCCTGTGCTGAAAGAATTAGCCATGACCAAACGTGGCATCATCATCTTTGTAGGTGCAACAG
GTACAGGTAAATCGACCTCTTTGGCTTCGATCATTGGTTACCGTAACCAAAACTCGAAAGGTCATATCATTACCATTGAA
GACCCAATTGAATTTATTCATGAGCATGCAGGCTGTATCATTACCCAGCGTGAAGTGGGCATTGATACAGACTCTTTTGA
AATCGCGCTGAAAAACACCTTACGTCAGGCACCCGATGTGATCCTGATTGGTGAGATTCGTTCCCGTGAAACCATGGATT
ATGCGATTGCCTTTGCTGAAACAGGTCACTTGGTATTTGCAACGCTGCACGCCAACAACGCCAACCAAGCCATCGACCGT
ATTATTCACTTCTTTGAAGCAGACCGTCATAGCCAATTGTTTATGGACTTGTCTTTAAACCTTAAAGCGATGGTTGCTCA
GCAGTTAATTCCAACACCTGACGGTAATTCACGTCGCGCTGCAATTGAGATTTTGATTAACTCACCGTTAATTTCCGATT
TGATCCGTAAAGGTGAAATCCACGAAATTAAAGACCTGATGAAGCGTTCTCGTGAACTCGGTATGCAAACCTTTGACCAA
GCCTTATTCGATTTGTACAAAGCTAAGCAGATTACCTATAAAGATGCGCTGAAACACGCCGACTCTCCAAACGACTTACG
CTTACAAATCAAGTTGTCTGAAGAAGGCGGTGATCGTCTATTGCATGCCAGTGCAAACATCACGTTTGACGGACAAGCTT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9BCS7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

76.72

100

0.777
  pilU Pseudomonas stutzeri DSM 10701

67.925

99.464

0.676
  pilU Vibrio cholerae strain A1552

55.405

99.196

0.55
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.197

92.761

0.391
  pilT Legionella pneumophila strain Lp02

41.42

90.617

0.375
  pilT Legionella pneumophila strain ERS1305867

41.42

90.617

0.375
  pilT Pseudomonas aeruginosa PAK

39.306

92.761

0.365