Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   TQH59_RS13215 Genome accession   NZ_CP140708
Coordinates   2659984..2661021 (+) Length   345 a.a.
NCBI ID   WP_004694862.1    Uniprot ID   N9CT59
Organism   Acinetobacter johnsonii strain FAHZZU3567hy     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2654984..2666021
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TQH59_RS13205 (TQH59_13205) - 2658250..2658984 (-) 735 WP_333640499.1 hypothetical protein -
  TQH59_RS13210 (TQH59_13210) - 2659175..2659861 (-) 687 WP_333640500.1 YggS family pyridoxal phosphate-dependent enzyme -
  TQH59_RS13215 (TQH59_13215) pilT 2659984..2661021 (+) 1038 WP_004694862.1 type IV pilus twitching motility protein PilT Machinery gene
  TQH59_RS13220 (TQH59_13220) pilU 2661048..2662169 (+) 1122 WP_004984301.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  TQH59_RS13225 (TQH59_13225) fur 2662230..2662667 (-) 438 WP_004694866.1 ferric iron uptake transcriptional regulator -
  TQH59_RS13230 (TQH59_13230) - 2662780..2663178 (+) 399 WP_004694868.1 outer membrane protein assembly factor BamE -
  TQH59_RS13235 (TQH59_13235) - 2663225..2663548 (-) 324 WP_333640501.1 RnfH family protein -
  TQH59_RS13240 (TQH59_13240) - 2663548..2664630 (-) 1083 WP_333640502.1 hypothetical protein -
  TQH59_RS13245 (TQH59_13245) - 2664773..2665228 (-) 456 WP_005400160.1 bacteriohemerythrin -
  TQH59_RS13250 (TQH59_13250) - 2665354..2665746 (-) 393 Protein_2568 transposase family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38292.96 Da        Isoelectric Point: 6.6658

>NTDB_id=915084 TQH59_RS13215 WP_004694862.1 2659984..2661021(+) (pilT) [Acinetobacter johnsonii strain FAHZZU3567hy]
MDITELLAFSAKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKEVHKLVYDIMNDKQRRDFEEKLETDFSFEVPSIAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQTFKELCELPRGLVLVTGPTGSGKSTTLAAMIDYINDNRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KNLVTKGVISPQTARTAAKQPETFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=915084 TQH59_RS13215 WP_004694862.1 2659984..2661021(+) (pilT) [Acinetobacter johnsonii strain FAHZZU3567hy]
ATGGATATCACAGAATTACTAGCCTTTTCGGCTAAGAACGGGGCATCAGATTTACACTTATCTGCAGGTATGCCACCGAT
GATTCGTGTCGATGGTGAAGTTCGTCGTATCAATTTACCAGCTTTAGAACACAAAGAAGTGCATAAACTGGTCTATGACA
TCATGAATGACAAACAACGTCGTGATTTCGAAGAAAAATTAGAAACGGACTTTTCCTTTGAAGTTCCAAGCATTGCTCGC
TTTCGTGTGAACGCATTCAACCAAAACCGCGGTGCAGGTGCAGTATTCCGTACCATTCCGTCTAAAGTCCTGACCATGGA
AGACTTAGGTTTAGGACAAACCTTTAAAGAGTTGTGTGAATTGCCACGTGGTTTAGTTTTAGTGACGGGGCCTACTGGTT
CTGGTAAATCTACCACGTTGGCAGCAATGATCGATTATATTAACGATAACCGCTATGATCACATCTTAACAGTTGAAGAC
CCCATCGAATTTGTACACCAGTCAAAAAAATGCTTGATCAACCAACGTGAAGTACACCGTGATACACATGGTTTTAACGA
AGCGTTGCGTTCTGCACTCCGTGAAGACCCCGATATTATCCTCGTGGGTGAGATGCGTGACCTTGAAACCATTCGTTTGG
CGCTCACTGCCGCTGAAACGGGTCACTTGGTCTTCGGTACACTACATACCACCTCTGCGGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCTGAAGAAAAAGACATGGTTCGTGCCATGTTATCTGAATCGCTACAAGCCGTGATTTCTCAAAC
CTTACTCAAGAAAAATGGCGGTGGCCGTGTTGCTGCGCATGAAATCATGATTGGTATTCCTGCCATTCGTAACTTAATTC
GTGAAAACAAAGTGGCACAGATGTATTCTGCTATTCAGACTGGTGCAAACTACGGTATGACCACACTGGATCAAAGTCTG
AAAAACTTGGTCACCAAAGGGGTGATCAGCCCACAGACTGCGCGTACAGCTGCCAAGCAACCTGAAACCTTCTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9CT59

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

94.493

100

0.945
  pilT Acinetobacter baumannii D1279779

94.493

100

0.945
  pilT Acinetobacter baumannii strain A118

94.493

100

0.945
  pilT Acinetobacter baylyi ADP1

86.337

99.71

0.861
  pilT Pseudomonas aeruginosa PAK

84.012

99.71

0.838
  pilT Pseudomonas stutzeri DSM 10701

83.43

99.71

0.832
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Neisseria meningitidis 8013

67.246

100

0.672
  pilT Neisseria gonorrhoeae MS11

66.957

100

0.67
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.452

97.391

0.667
  pilT Vibrio cholerae strain A1552

68.452

97.391

0.667
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.525
  pilU Vibrio cholerae strain A1552

42.183

98.261

0.414
  pilU Pseudomonas stutzeri DSM 10701

42.09

97.101

0.409
  pilU Acinetobacter baylyi ADP1

39.942

99.42

0.397