Detailed information    

experimental Experimentally validated

Overview

Name   fimV   Type   Machinery gene
Locus tag   PAKAF_RS09365 Genome accession   NZ_LR657304
Coordinates   1992909..1995668 (+) Length   919 a.a.
NCBI ID   WP_016253171.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa PAK     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

P. aeruginosa in biofilms is competent for natural transformation of both genomic and plasmid DNA. All T4P mutants were capable of some natural transformation of pUCPSK, however a significant reduction in transformation frequency compared to wild-type PAK was observed.


Genomic Context

Location: 1987909..2000668
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PAKAF_RS09345 (PAKAF_01864) - 1988544..1989305 (+) 762 WP_003091396.1 class I SAM-dependent methyltransferase -
  PAKAF_RS09350 (PAKAF_01865) leuB 1989364..1990446 (+) 1083 WP_003091395.1 3-isopropylmalate dehydrogenase -
  PAKAF_RS09355 (PAKAF_01866) asd 1990516..1991628 (+) 1113 WP_003111187.1 aspartate-semialdehyde dehydrogenase -
  PAKAF_RS09360 (PAKAF_01867) - 1991730..1992740 (+) 1011 WP_003115016.1 aspartate-semialdehyde dehydrogenase -
  PAKAF_RS09365 (PAKAF_01868) fimV 1992909..1995668 (+) 2760 WP_016253171.1 motility hub landmark protein FimV Machinery gene
  PAKAF_RS09370 (PAKAF_01869) truA 1995690..1996547 (+) 858 WP_003091390.1 tRNA pseudouridine(38-40) synthase TruA -
  PAKAF_RS09375 (PAKAF_01870) - 1996637..1997272 (+) 636 WP_003116445.1 phosphoribosylanthranilate isomerase -
  PAKAF_RS09380 (PAKAF_01871) accD 1997541..1998413 (+) 873 WP_003104199.1 acetyl-CoA carboxylase, carboxyltransferase subunit beta -
  PAKAF_RS09385 (PAKAF_01872) folC 1998410..1999699 (+) 1290 WP_003115014.1 bifunctional tetrahydrofolate synthase/dihydrofolate synthase -
  PAKAF_RS09390 (PAKAF_01873) - 1999703..2000368 (+) 666 WP_016253172.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 919 a.a.        Molecular weight: 96959.20 Da        Isoelectric Point: 3.9481

>NTDB_id=1196 PAKAF_RS09365 WP_016253171.1 1992909..1995668(+) (fimV) [Pseudomonas aeruginosa PAK]
MVRLRTLVRAIAAASVLTSGMAHGLGLGEITLKSALNQPLDAEIELLEVRDLGSGEVIPSLASPEEFSKAGVDRLYYLTD
LKFTPVVKPNGKSVIRVTSSKPVQEPYLNFLVQVLWPNGRLLREYTVLLDPPLYSPQAAASAPQAPVSAPRATGAPRAPQ
APAPVRTTAPAGSDTYRTVSNDTLWEIAQRNRTDRVSVPQAMLAFQELNPGAFVDGNINRLKSGQVLRIPTEQQMLERSP
REALSQVQAQNQSWRGSRNPAAGSAGARQLDATQRNAAGSAPSKVDATDNLRLVSGEGKASKGADKGGKGDSKAIADTLA
VTKESLDSTRRENEELQSRMQDLQSQLDKLQKLIQLKDAQLAKLQGQLGAEGQGAAQPNAALPDASQPNAAAQAPAQPGT
PAVAAPTPAPAGEAPAAPAQPPVAPPPAPAAERPPAPAVPAPAPVQAAEQPAPSFLDELLANPLWLAVIGGSALLALLVL
LMILSRRNAQKEKEEAQAFAADTGEEQEDALDLGKDGFDDLTLDEPEPQVAAVAPQVEKTTAQTSDALGEADIYIAYGRF
NQAAELLQNAIYDEPQRTDLRLKLMEVYAEMGDREGFARQENELREIGGAQPQVEQLKSRYPAMVAVAAVAGLAGAKLAQ
DELDSFSLDDLSLDDSGHAAKPDAAGQDLDDAFDLSLDDLGGDDVQADLKSDSGALDDLTLDSDLDLAASTAADKPVDDL
DFGLDFAELAETPSQPKHDDLGDFSLDLDAPEDKLSDDDFLLSLNDEVPAAAPADNEFTLDTEAAEEPALSLPDDFDLSL
ADEPTEPAAPEKGEDSFAAQLDEVSAQLDELASNLDEPKSATPSFSAEDAAVASALDGDADDDFDFLSGADEAATKLDLA
RAYIDMGDSEGARDILDEVLAEGNDSQQAEARELLERLA

Nucleotide


Download         Length: 2760 bp        

>NTDB_id=1196 PAKAF_RS09365 WP_016253171.1 1992909..1995668(+) (fimV) [Pseudomonas aeruginosa PAK]
ATGGTTCGGCTTCGTACACTGGTTCGGGCAATCGCGGCGGCCTCGGTCCTGACTTCCGGCATGGCGCATGGATTGGGACT
GGGGGAAATCACCCTGAAGTCGGCGTTGAACCAACCGTTGGATGCCGAGATCGAGCTGCTCGAAGTTCGTGACCTGGGTT
CGGGCGAGGTGATCCCGAGCCTGGCGTCGCCGGAAGAGTTCAGCAAGGCGGGCGTCGATCGCCTGTACTACCTCACCGAC
CTGAAGTTCACGCCGGTGGTGAAGCCCAACGGCAAGAGCGTCATTCGCGTGACCTCGTCGAAGCCGGTGCAGGAGCCCTA
CCTGAACTTCCTGGTCCAGGTGCTCTGGCCGAACGGCCGCCTGCTGCGCGAGTACACCGTCCTGCTGGATCCGCCGCTGT
ACTCCCCGCAGGCCGCGGCAAGCGCTCCGCAAGCGCCGGTCAGCGCGCCGCGCGCGACCGGCGCCCCGCGAGCCCCGCAG
GCTCCGGCTCCGGTGCGTACCACCGCGCCGGCAGGCAGCGACACCTATCGCACCGTTTCCAACGACACGCTCTGGGAGAT
CGCCCAGCGCAACCGTACCGATCGCGTTTCCGTACCCCAGGCGATGCTCGCGTTCCAGGAGCTGAATCCGGGCGCCTTCG
TCGATGGCAACATCAACCGGCTGAAGAGCGGCCAGGTCCTGCGCATTCCCACCGAACAGCAGATGCTGGAGCGCTCGCCG
CGCGAGGCGCTGTCCCAGGTGCAGGCGCAGAACCAGAGCTGGCGCGGCAGCCGCAATCCGGCCGCGGGCAGCGCTGGCGC
CAGGCAGTTGGATGCGACCCAGCGCAATGCCGCCGGGTCGGCGCCATCCAAGGTCGACGCCACGGACAATCTGCGCCTGG
TGTCTGGCGAGGGCAAGGCCAGCAAGGGTGCCGACAAGGGCGGAAAGGGCGACAGCAAGGCGATCGCCGATACCCTGGCG
GTGACCAAGGAAAGCCTCGACAGCACTCGCCGCGAGAACGAAGAACTGCAGAGTCGCATGCAGGATCTGCAGAGCCAGTT
GGACAAGCTGCAGAAGTTGATCCAGCTGAAGGACGCCCAGTTGGCCAAGCTGCAAGGGCAGTTGGGCGCCGAAGGCCAGG
GCGCAGCCCAGCCGAACGCAGCCCTGCCGGATGCGTCCCAGCCCAATGCGGCCGCGCAGGCGCCGGCTCAGCCCGGGACT
CCTGCTGTGGCAGCGCCGACTCCTGCTCCAGCCGGAGAAGCACCCGCCGCTCCGGCGCAGCCTCCGGTGGCGCCGCCGCC
CGCGCCAGCTGCCGAGAGGCCTCCGGCACCTGCCGTTCCGGCGCCCGCTCCGGTACAGGCGGCAGAGCAGCCGGCACCGA
GCTTCCTCGACGAACTGCTGGCCAACCCGCTGTGGTTGGCGGTGATCGGCGGTAGCGCACTGCTGGCGTTGCTGGTGCTG
CTGATGATCCTGTCGCGGCGCAATGCGCAGAAAGAGAAGGAAGAAGCCCAGGCTTTCGCCGCGGATACCGGCGAGGAACA
GGAGGATGCGCTGGACCTGGGAAAGGACGGCTTCGACGACCTGACCCTCGACGAGCCTGAGCCGCAGGTCGCAGCCGTCG
CTCCGCAGGTCGAGAAGACCACCGCGCAGACTTCCGATGCGCTGGGCGAGGCCGACATCTATATCGCCTACGGGCGTTTC
AACCAGGCCGCCGAACTGTTGCAGAACGCCATCTACGACGAGCCGCAGCGCACCGACCTGCGCCTCAAGCTGATGGAAGT
CTATGCCGAGATGGGCGATCGCGAAGGTTTCGCTCGCCAGGAAAACGAGCTGCGCGAAATCGGCGGCGCACAGCCGCAGG
TCGAGCAGCTCAAGTCGCGCTATCCGGCAATGGTCGCGGTCGCCGCGGTTGCCGGCCTGGCCGGCGCCAAGCTGGCGCAG
GACGAGCTGGATAGCTTCAGCCTCGACGATCTGTCGCTCGACGACAGCGGTCACGCGGCCAAGCCGGATGCGGCAGGACA
GGATCTCGACGACGCCTTCGACCTGAGCCTGGACGACCTGGGCGGCGACGACGTGCAGGCCGACCTCAAGTCCGACAGCG
GGGCGCTGGACGACCTGACCCTGGACAGCGATCTGGACCTGGCGGCCTCGACCGCGGCGGACAAGCCTGTCGACGATCTC
GACTTCGGCCTGGATTTCGCGGAGTTGGCAGAGACTCCGAGCCAACCCAAGCATGACGACCTGGGCGATTTCTCCCTGGA
TCTCGATGCGCCGGAAGACAAGCTTTCGGACGACGACTTCCTGCTTTCGCTGAACGACGAAGTGCCCGCCGCGGCGCCCG
CCGACAACGAATTCACCCTCGATACCGAGGCTGCCGAAGAGCCGGCGTTGTCCCTGCCGGACGACTTCGACCTGTCGCTG
GCCGACGAGCCGACGGAGCCGGCCGCTCCGGAGAAGGGCGAGGACAGTTTCGCCGCCCAGTTGGACGAGGTGAGTGCGCA
GTTGGACGAGTTGGCCAGCAACCTTGACGAGCCGAAGAGCGCGACGCCGAGTTTCTCCGCCGAAGATGCAGCGGTCGCCT
CCGCCCTGGACGGAGACGCCGACGATGACTTCGACTTCCTCTCCGGTGCCGACGAAGCGGCGACCAAGCTGGATCTGGCT
CGCGCCTACATCGACATGGGCGATAGCGAAGGCGCGCGCGATATCCTCGACGAAGTCCTGGCCGAAGGTAACGACAGCCA
GCAGGCGGAAGCCCGCGAGTTGCTGGAGCGCCTGGCCTGA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Laura M Nolan et al. (2020) Pseudomonas aeruginosa is capable of natural transformation in biofilms. Microbiology (Reading, England) 166(10):995-1003. [PMID: 32749953]