Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   TQH59_RS01610 Genome accession   NZ_CP140708
Coordinates   327879..329597 (-) Length   572 a.a.
NCBI ID   WP_333641083.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain FAHZZU3567hy     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 322879..334597
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TQH59_RS01580 (TQH59_01580) - 322977..323300 (+) 324 WP_125273379.1 pyrimidine/purine nucleoside phosphorylase -
  TQH59_RS01585 (TQH59_01585) rlmB 323443..324192 (+) 750 WP_004978746.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  TQH59_RS01590 (TQH59_01590) - 324224..325141 (+) 918 WP_171428537.1 DMT family transporter -
  TQH59_RS01595 (TQH59_01595) coaE 325156..325755 (-) 600 WP_125273378.1 dephospho-CoA kinase -
  TQH59_RS01600 (TQH59_01600) pilD 325762..326622 (-) 861 WP_125273377.1 prepilin peptidase Machinery gene
  TQH59_RS01605 (TQH59_01605) pilC 326622..327848 (-) 1227 WP_010325767.1 type II secretion system F family protein Machinery gene
  TQH59_RS01610 (TQH59_01610) pilB 327879..329597 (-) 1719 WP_333641083.1 type IV-A pilus assembly ATPase PilB Machinery gene
  TQH59_RS01615 (TQH59_01615) tpiA 329877..330668 (+) 792 WP_333641085.1 triose-phosphate isomerase -
  TQH59_RS01620 (TQH59_01620) secG 330682..331011 (+) 330 WP_004692710.1 preprotein translocase subunit SecG -
  TQH59_RS01650 (TQH59_01650) rimP 331965..332489 (+) 525 WP_004692708.1 ribosome maturation factor RimP -
  TQH59_RS01655 (TQH59_01655) nusA 332529..334013 (+) 1485 WP_004692706.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 63835.12 Da        Isoelectric Point: 4.9579

>NTDB_id=915069 TQH59_RS01610 WP_333641083.1 327879..329597(-) (pilB) [Acinetobacter johnsonii strain FAHZZU3567hy]
MSVSNGLPKFTGFIRRLVEDGSMSAENMQQAMLSAKKAQQDIVPYLIENHKLSPQMIAETISLEFGEPVFDLSVYDSAQV
LRDLVEDKLITKHRVLPIYRRGNILYIATSNPTNMDAMDAIRFNSKMNVEAIIVEHPQLEKLIEQHFTKADNFEFEDEDI
DLDLDLEQDHQNSNDEEDDSPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRHIASPPLQLATRL
ASRLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKDLFMQALD
KPQGMLLITGPTGSGKTVSLYTGLNILNKEDTNISTAEDPVEINLEGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGE
IRDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKAAVDIPEQSLLEM
GFTSTDIKDPDFKVYQPVGCTECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAEASAKLGFNNLRRSGLLKVMQGV
TSLQEVNRVTSE

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=915069 TQH59_RS01610 WP_333641083.1 327879..329597(-) (pilB) [Acinetobacter johnsonii strain FAHZZU3567hy]
ATGTCAGTATCAAATGGTTTGCCTAAGTTCACAGGCTTCATTCGACGCTTAGTCGAAGACGGAAGCATGTCTGCTGAAAA
TATGCAGCAGGCAATGCTCTCTGCAAAAAAGGCTCAGCAAGACATCGTTCCTTATCTGATTGAAAATCATAAACTCTCCC
CACAAATGATCGCTGAAACGATTTCTTTAGAGTTTGGCGAGCCCGTTTTTGACTTAAGTGTCTATGATTCTGCACAAGTT
CTACGGGATCTGGTCGAAGACAAACTCATCACCAAACATCGTGTACTACCAATTTACCGTCGAGGCAATATTCTCTATAT
CGCTACCAGCAATCCAACCAATATGGATGCGATGGACGCGATTCGCTTCAATTCTAAAATGAACGTCGAAGCAATCATTG
TTGAACATCCTCAGCTTGAGAAATTGATTGAGCAACACTTTACTAAAGCAGATAACTTTGAGTTCGAAGATGAAGATATT
GATCTAGATTTAGACTTAGAACAAGACCATCAGAACTCAAATGACGAAGAAGATGACTCACCTCAAGGCGATGAAGCGCC
GATCGTCAAATACATCAATAAATTACTGATCGATGCGATTCGTATGGGCGCCTCAGACTTACACTTTGAACCTTATGAAA
AATCGTATCGTGTTCGTTATCGTGTAGATGGTGTTCTGCGTCATATTGCCAGTCCACCACTTCAACTGGCAACTCGTCTT
GCCTCACGTTTAAAAGTTATGTCACAAATGGACATCTCTGAAAAGCGCGTCCCGCAAGATGGCCGAATTAAATTAAAGCT
GTCGAAAAGCAAAGCCATCGACTTCCGTGTCAACTCACTTCCAACCTTATTTGGCGAAAAGCTGGTTCTACGTATTCTCG
ATCCATCCAGTGCTATGCTGGGGATTGATGCTTTAGGTTATGAACCAGATCAAAAAGACCTATTTATGCAGGCTTTAGAC
AAACCGCAAGGCATGTTGTTAATTACTGGCCCGACAGGTTCAGGTAAAACGGTATCGCTATATACAGGTTTGAATATTCT
AAATAAAGAAGACACTAATATCTCAACTGCAGAAGACCCCGTTGAGATTAACTTAGAAGGTATTAATCAGGTCAATGTGA
ACAATAAAGTCGGCCTCACCTTCTCTGCTGCGCTGAAGTCCTTCTTACGTCAGGATCCTGATATTGTAATGGTGGGTGAG
ATTCGTGACCTTGAAACGGCTGAAATCGCGATTAAAGCCGCACAGACAGGCCACATGGTCATGTCGACACTCCACACCAA
TAGTGCACCTGAAACTCTGACACGCTTACGCAATATGGGGGTTCCATCTTTTAACATTGCGACATCGGTCAATTTGGTAA
TTGCACAGCGTTTGGCACGCCGTTTATGTAGCCAATGCAAAGCCGCTGTCGATATTCCTGAACAAAGCTTGCTCGAAATG
GGCTTTACTTCAACAGATATCAAAGATCCAGACTTCAAAGTTTATCAACCGGTGGGATGTACTGAGTGCCGTGAAGGCTA
TAAAGGACGTGTTGGTATTTATGAAGTCATGAAAGTCACACCAGAAATTTCAAAAATTATTATGGAAGATGGCAATGCCT
TACAGATTGCGGAAGCATCTGCAAAATTAGGATTTAACAATTTACGTCGTTCAGGTTTACTCAAGGTTATGCAGGGGGTC
ACTTCCTTACAAGAAGTGAATCGTGTAACCAGCGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-148)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.119

100

0.811
  pilB Acinetobacter baylyi ADP1

77.098

100

0.771
  pilB Legionella pneumophila strain ERS1305867

53.558

93.357

0.5
  pilF Neisseria gonorrhoeae MS11

49.823

98.601

0.491
  pilB Vibrio cholerae strain A1552

49.733

98.077

0.488
  pilB Vibrio campbellii strain DS40M4

47.865

98.252

0.47
  pilB Vibrio parahaemolyticus RIMD 2210633

50.867

90.734

0.462
  pilF Thermus thermophilus HB27

36.475

100

0.365
  pilB/pilB1 Synechocystis sp. PCC 6803

33.659

100

0.362