Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   U2S75_RS18170 Genome accession   NZ_CP140420
Coordinates   3800546..3801031 (-) Length   161 a.a.
NCBI ID   WP_000993726.1    Uniprot ID   A0A242SN80
Organism   Acinetobacter baumannii strain 2023CK-00893     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3795546..3806031
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U2S75_RS18150 (U2S75_18145) - 3797205..3797588 (+) 384 WP_000090000.1 RidA family protein -
  U2S75_RS18155 (U2S75_18150) - 3797798..3797992 (+) 195 WP_000289091.1 bacterioferritin-associated ferredoxin -
  U2S75_RS18160 (U2S75_18155) bfr 3798237..3798701 (+) 465 WP_000678123.1 bacterioferritin -
  U2S75_RS18165 (U2S75_18160) - 3798742..3800370 (-) 1629 WP_064193099.1 PglL family O-oligosaccharyltransferase -
  U2S75_RS18170 (U2S75_18165) pilA 3800546..3801031 (-) 486 WP_000993726.1 pilin Machinery gene
  U2S75_RS18175 (U2S75_18170) - 3801373..3801933 (-) 561 WP_016653486.1 TPM domain-containing protein -
  U2S75_RS18180 (U2S75_18175) - 3801927..3803009 (-) 1083 WP_072688987.1 YgcG family protein -
  U2S75_RS18185 (U2S75_18180) - 3803031..3803621 (-) 591 WP_000846931.1 LemA family protein -
  U2S75_RS18190 (U2S75_18185) - 3803752..3804639 (-) 888 WP_322546578.1 metal-dependent hydrolase -

Sequence

Protein


Download         Length: 161 a.a.        Molecular weight: 16591.91 Da        Isoelectric Point: 5.0937

>NTDB_id=914196 U2S75_RS18170 WP_000993726.1 3800546..3801031(-) (pilA) [Acinetobacter baumannii strain 2023CK-00893]
MNAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVGRSNVAAAVQTLTSNKSGLESYVMEFGEFPDGTTAPAAGDPTANPP
VAATRGERPQDLGIVNTTLGSIALGDKNNGAGIITLTFATGNPGIKGKKVQLARDANGTWTCVTDVDEKFAAKACPVGTP
Q

Nucleotide


Download         Length: 486 bp        

>NTDB_id=914196 U2S75_RS18170 WP_000993726.1 3800546..3801031(-) (pilA) [Acinetobacter baumannii strain 2023CK-00893]
ATGAATGCACAAAAAGGTTTTACATTAATCGAACTCATGATCGTGGTTGCCATTATCGGTATTTTGGCAGCAATTGCGAT
TCCGCAATATCAAAATTATGTGGGTCGCTCTAATGTAGCAGCAGCTGTCCAAACACTTACATCAAATAAATCTGGTTTAG
AAAGTTATGTAATGGAATTTGGTGAATTCCCTGACGGTACTACGGCTCCAGCTGCAGGAGATCCAACAGCGAATCCACCA
GTAGCTGCTACTCGAGGTGAGCGACCACAGGATTTAGGTATTGTTAATACAACATTAGGAAGTATTGCTTTAGGTGATAA
AAACAATGGAGCTGGCATTATCACTTTAACATTTGCGACAGGCAATCCTGGTATTAAAGGCAAAAAGGTTCAGTTAGCTC
GTGATGCAAATGGAACTTGGACATGTGTAACTGATGTTGATGAGAAATTTGCAGCCAAAGCTTGTCCAGTAGGAACACCA
CAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A242SN80

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

48.75

99.379

0.484
  pilA Pseudomonas aeruginosa PAK

46.541

98.758

0.46
  pilA Vibrio cholerae C6706

45.86

97.516

0.447
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

45.86

97.516

0.447
  pilA Vibrio cholerae strain A1552

45.86

97.516

0.447
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.558

95.652

0.398
  pilE Neisseria gonorrhoeae MS11

37.736

98.758

0.373
  comP Acinetobacter baylyi ADP1

36.709

98.137

0.36