Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   R5R73_RS07920 Genome accession   NZ_CP140261
Coordinates   1699027..1700754 (-) Length   575 a.a.
NCBI ID   WP_322529370.1    Uniprot ID   -
Organism   Salinicola sp. LHM     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1694027..1705754
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R5R73_RS07895 (R5R73_07895) - 1694402..1695355 (+) 954 WP_071232858.1 Nudix family hydrolase -
  R5R73_RS07900 (R5R73_07900) yacG 1695591..1695869 (-) 279 WP_322529367.1 DNA gyrase inhibitor YacG -
  R5R73_RS07905 (R5R73_07905) coaE 1695914..1696567 (-) 654 WP_322529368.1 dephospho-CoA kinase -
  R5R73_RS07910 (R5R73_07910) pilD 1696564..1697424 (-) 861 WP_322529369.1 A24 family peptidase Machinery gene
  R5R73_RS07915 (R5R73_07915) pilC 1697658..1698911 (-) 1254 WP_071232856.1 type II secretion system F family protein Machinery gene
  R5R73_RS07920 (R5R73_07920) pilB 1699027..1700754 (-) 1728 WP_322529370.1 type IV-A pilus assembly ATPase PilB Machinery gene
  R5R73_RS07925 (R5R73_07925) - 1701209..1701736 (+) 528 WP_322529371.1 pilin -
  R5R73_RS07930 (R5R73_07930) - 1701857..1702141 (-) 285 WP_322529372.1 hypothetical protein -
  R5R73_RS07935 (R5R73_07935) - 1702523..1703974 (+) 1452 WP_322529373.1 TrkH family potassium uptake protein -
  R5R73_RS07940 (R5R73_07940) - 1704036..1704815 (-) 780 WP_322529374.1 RIO1 family regulatory kinase/ATPase -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63222.32 Da        Isoelectric Point: 4.9264

>NTDB_id=913314 R5R73_RS07920 WP_322529370.1 1699027..1700754(-) (pilB) [Salinicola sp. LHM]
MSDVTTSRTQSSLAQRFIEHGLISAEACEQAIRDAADRQLTLLELVVREGRVDAREAGELAAREYGLPFMDLEHVDWDKL
PIIEPVADKVIRRFQVLPLARDDRRLTVAIAFPVTLAQLDELQFTTGLAIEGVLVPFDQLEQALAKRYQRHETSGMLEAL
GEASEALTLDTSAADLASEAAALDQIGEDDEPVVRFVNKILHDAIQRGASDIHFEPYESSYRIRFRIDGLLLEIARPPLS
LRTRLAARLKVMARLDISERRLPQDGSMKIRLDSERAIDFRVNTLPTVFGEKIVLRLLDARTASFGIDALGFTPEQRQLF
EAALAEPQGMILATGPTGSGKTVTLYTGINLLNTEERNISTAEDPVELKVPGVNQVNVQPRIGLDFASALRAFLRQDPDV
VMVGEIRDLETAEIAVKAAQTGHLVLSTLHTNSAAETLTRLRNMGVASFNIASSVSLIIAQRLARMLCPRCKAPAEIPED
VLRHEGVSPERLASATLYRAVGCDRCTLGYKGRIGIYEVVPVDDAMSRLIMADSHALELARAARDAGFPGLRQSGIDKVL
QGVTSLEEINRVTID

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=913314 R5R73_RS07920 WP_322529370.1 1699027..1700754(-) (pilB) [Salinicola sp. LHM]
ATGTCGGACGTCACCACATCGCGTACGCAAAGCAGTCTTGCCCAGCGCTTCATCGAACACGGCCTGATCAGTGCGGAGGC
GTGCGAGCAGGCGATCCGCGACGCGGCGGACAGGCAGCTGACGCTGCTGGAGCTGGTGGTCCGGGAAGGCAGGGTCGATG
CCCGCGAAGCCGGCGAGCTGGCAGCGCGCGAGTACGGCCTGCCGTTCATGGATCTGGAGCACGTCGACTGGGACAAGCTG
CCGATCATCGAGCCGGTGGCGGACAAGGTCATCCGCCGCTTTCAGGTGCTGCCGCTGGCCCGCGACGATCGCCGCCTCAC
CGTGGCGATCGCCTTCCCGGTCACGCTGGCACAGCTGGACGAGCTGCAGTTCACCACCGGGCTTGCGATCGAGGGCGTTC
TGGTGCCGTTCGATCAACTGGAGCAGGCGCTGGCCAAGCGTTACCAGCGCCACGAAACCTCCGGCATGCTGGAAGCGCTG
GGGGAAGCCAGCGAGGCGCTGACGCTGGATACCAGCGCGGCCGACCTGGCCAGCGAAGCGGCCGCGCTCGACCAGATCGG
GGAAGACGACGAGCCCGTGGTGCGCTTCGTCAACAAGATCCTGCACGACGCGATCCAGCGCGGCGCCTCCGATATCCACT
TCGAGCCCTACGAGTCGAGCTACCGGATTCGCTTTCGCATCGACGGGTTGCTGCTCGAGATCGCGCGACCGCCGCTGAGC
TTGCGCACCCGCCTCGCCGCCCGGCTCAAGGTCATGGCGCGACTGGATATTTCGGAGCGCCGCCTGCCCCAGGACGGCTC
GATGAAGATTCGGCTCGATAGTGAGCGTGCCATCGATTTCCGCGTCAACACGCTGCCCACCGTGTTCGGCGAGAAAATCG
TCCTGCGACTGCTGGATGCCCGAACGGCCAGCTTCGGCATCGATGCGCTGGGCTTCACCCCGGAGCAGCGTCAACTGTTC
GAGGCCGCGCTGGCCGAGCCCCAGGGAATGATTCTGGCCACCGGCCCCACCGGTAGCGGCAAGACGGTAACGCTCTATAC
CGGCATCAATCTGCTCAATACCGAGGAGCGCAATATCTCGACCGCGGAAGATCCGGTGGAACTCAAGGTGCCCGGCGTCA
ACCAGGTCAACGTGCAGCCACGCATCGGCCTCGACTTCGCCAGCGCGCTGCGCGCCTTTCTGCGCCAGGATCCGGACGTG
GTGATGGTCGGCGAAATCCGGGATCTGGAAACGGCCGAAATCGCGGTCAAGGCAGCCCAGACCGGCCACCTGGTGCTATC
GACGCTGCATACCAACTCCGCCGCCGAAACCCTGACGCGCCTGCGCAACATGGGCGTTGCCAGCTTCAATATCGCCAGTT
CGGTATCGCTGATCATTGCCCAGCGGCTGGCGCGTATGCTCTGCCCGCGCTGCAAGGCGCCGGCGGAGATTCCCGAGGAC
GTGTTGCGCCACGAAGGCGTCTCGCCGGAGAGACTGGCAAGCGCCACTCTCTACCGCGCCGTGGGCTGCGATCGGTGCAC
GCTGGGCTACAAGGGGCGCATCGGGATCTACGAGGTGGTGCCGGTGGACGATGCCATGAGCCGGCTGATCATGGCCGACA
GTCACGCGCTGGAGCTGGCCCGCGCGGCACGCGATGCCGGCTTCCCGGGGCTGCGCCAGAGCGGTATCGACAAGGTACTT
CAAGGCGTGACCAGCCTCGAGGAGATCAACCGCGTGACGATCGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(199-466)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

51.826

100

0.518
  pilB Acinetobacter baumannii D1279779

51.042

100

0.511
  pilB Legionella pneumophila strain ERS1305867

52.399

94.261

0.494
  pilB Vibrio parahaemolyticus RIMD 2210633

49.824

98.783

0.492
  pilB Vibrio campbellii strain DS40M4

49.192

96.87

0.477
  pilB Vibrio cholerae strain A1552

50.562

92.87

0.47
  pilF Neisseria gonorrhoeae MS11

45.664

98.261

0.449
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.339

98.435

0.377
  pilF Thermus thermophilus HB27

37.543

100

0.377
  pilB/pilB1 Synechocystis sp. PCC 6803

40.115

90.609

0.363