Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   U0040_RS14870 Genome accession   NZ_CP140003
Coordinates   3001034..3002746 (+) Length   570 a.a.
NCBI ID   WP_114837769.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain HAMBI_0097     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2996034..3007746
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U0040_RS14825 (U0040_14820) nusA 2996619..2998103 (-) 1485 WP_004692706.1 transcription termination factor NusA -
  U0040_RS14830 (U0040_14825) rimP 2998143..2998667 (-) 525 WP_004692708.1 ribosome maturation factor RimP -
  U0040_RS14860 (U0040_14855) secG 2999620..2999949 (-) 330 WP_058868796.1 preprotein translocase subunit SecG -
  U0040_RS14865 (U0040_14860) tpiA 2999963..3000754 (-) 792 WP_114837770.1 triose-phosphate isomerase -
  U0040_RS14870 (U0040_14865) pilB 3001034..3002746 (+) 1713 WP_114837769.1 type IV-A pilus assembly ATPase PilB Machinery gene
  U0040_RS14875 (U0040_14870) pilC 3002777..3004003 (+) 1227 WP_114837768.1 type II secretion system F family protein Machinery gene
  U0040_RS14880 (U0040_14875) pilD 3004003..3004863 (+) 861 WP_114837767.1 prepilin peptidase Machinery gene
  U0040_RS14885 (U0040_14880) coaE 3004872..3005471 (+) 600 WP_114837766.1 dephospho-CoA kinase -
  U0040_RS14890 (U0040_14885) - 3005485..3006402 (-) 918 WP_171355094.1 DMT family transporter -
  U0040_RS14895 (U0040_14890) rlmB 3006435..3007184 (-) 750 WP_114837765.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  U0040_RS14900 (U0040_14895) - 3007327..3007650 (-) 324 WP_004692718.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63616.97 Da        Isoelectric Point: 4.8447

>NTDB_id=912361 U0040_RS14870 WP_114837769.1 3001034..3002746(+) (pilB) [Acinetobacter johnsonii strain HAMBI_0097]
MSVSNGLPKFTGFIRRLVEDGRMPAENMQQAMLSAKKAQQDIVPYLIENYKLSPQMIAETISLEFGEPVFDLSVYDSVQI
LRDLVEDRLITKHRVLPIYRRGNILYIATSNPTNMDAMDAIRFNSKMNIEAIIVEYPQLEKLIEQNFAQADSFEFSDEDI
DLDLEQDHQNSNDEEDDSPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRHIASPPLQLATRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKDLFMQALDKP
QGMLLITGPTGSGKTVSLYTGLNILNKEDTNISTAEDPVEINLEGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKAAVDIPEQSLLEMGF
TSTDIKDPDFKVYQPVGCAECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAEASAKLGFNNLRCSGLLKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=912361 U0040_RS14870 WP_114837769.1 3001034..3002746(+) (pilB) [Acinetobacter johnsonii strain HAMBI_0097]
ATGTCAGTATCAAATGGTTTGCCTAAGTTCACAGGCTTCATTCGACGCTTAGTCGAAGACGGACGCATGCCTGCTGAAAA
TATGCAGCAGGCAATGCTCTCTGCAAAAAAAGCTCAGCAAGACATCGTTCCTTATCTGATTGAAAATTATAAACTCTCCC
CACAAATGATCGCTGAGACGATTTCTTTGGAGTTTGGAGAGCCTGTTTTTGACTTAAGTGTCTATGATTCGGTACAAATT
CTACGAGATCTGGTCGAAGATAGACTTATCACCAAGCATCGTGTGTTGCCGATTTACCGTCGTGGCAATATCCTCTATAT
CGCCACCAGCAATCCAACCAACATGGATGCAATGGACGCGATTCGTTTCAATTCTAAAATGAACATTGAAGCGATTATTG
TTGAATATCCTCAGCTTGAAAAACTAATCGAACAAAATTTCGCGCAAGCCGATAGCTTTGAATTTTCTGATGAAGATATT
GATTTAGACTTAGAGCAAGACCATCAGAACTCCAATGACGAAGAAGATGACTCGCCTCAAGGGGATGAAGCACCGATCGT
CAAATACATCAATAAATTACTGATTGATGCGATTCGTATGGGCGCATCAGACTTGCACTTTGAACCTTATGAAAAATCTT
ACCGTGTCCGTTATCGAGTAGATGGGGTTCTGCGTCATATTGCCAGTCCACCACTACAACTGGCGACTCGTCTTGCCTCA
CGTTTAAAAGTTATGTCACAAATGGACATCTCTGAAAAGCGCGTCCCGCAAGATGGCCGAATTAAATTAAAGCTGTCGAA
AAGCAAAGCCATCGACTTCCGTGTCAACTCACTTCCAACCTTATTTGGCGAAAAGCTGGTTCTACGTATTCTCGATCCAT
CCAGTGCTATGCTGGGGATTGATGCTTTAGGTTATGAACCAGATCAAAAAGACCTATTTATGCAGGCTTTAGACAAACCG
CAAGGCATGTTGTTAATTACTGGCCCGACAGGTTCAGGTAAAACGGTATCGCTGTATACAGGTTTGAATATTCTAAATAA
AGAAGACACCAATATTTCAACTGCGGAAGACCCTGTTGAGATTAACTTAGAAGGCATTAATCAGGTCAATGTGAACAATA
AAGTCGGCCTCACCTTCTCTGCTGCGCTGAAGTCCTTCTTACGTCAAGATCCTGATATTGTGATGGTTGGTGAGATTCGC
GACCTTGAAACAGCTGAAATCGCAATTAAAGCTGCACAGACAGGTCACATGGTCATGTCGACACTCCACACCAATAGTGC
ACCTGAAACTCTGACACGCTTACGTAATATGGGGGTTCCATCTTTTAACATTGCGACATCGGTCAATTTGGTGATTGCAC
AGCGTTTGGCACGCCGCTTATGCAGCCAATGCAAAGCCGCTGTCGATATTCCTGAACAAAGTTTGCTCGAAATGGGCTTT
ACTTCCACAGATATCAAAGATCCAGATTTCAAAGTTTATCAACCGGTGGGGTGTGCTGAGTGCCGTGAAGGCTATAAAGG
ACGTGTTGGTATTTATGAAGTCATGAAAGTCACGCCAGAAATTTCAAAAATTATTATGGAAGATGGCAATGCCTTACAGA
TTGCGGAAGCATCTGCAAAATTAGGATTTAACAATTTACGTTGTTCAGGTTTACTCAAGGTTATGCAGGGGGTCACTTCC
TTACAAGAAGTGAATCGTGTAACCAGCGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.702

100

0.807
  pilB Acinetobacter baylyi ADP1

76.14

100

0.761
  pilB Legionella pneumophila strain ERS1305867

53.745

93.684

0.504
  pilF Neisseria gonorrhoeae MS11

49.466

98.596

0.488
  pilB Vibrio cholerae strain A1552

48.501

99.474

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

50.677

90.702

0.46
  pilB Vibrio campbellii strain DS40M4

50

90.877

0.454
  pilB/pilB1 Synechocystis sp. PCC 6803

34.839

100

0.379
  pilF Thermus thermophilus HB27

36.888

100

0.37