Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   U0024_RS04175 Genome accession   NZ_CP139994
Coordinates   916516..917733 (-) Length   405 a.a.
NCBI ID   WP_304352032.1    Uniprot ID   -
Organism   Comamonas testosteroni strain HAMBI_0403     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 911516..922733
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U0024_RS04145 (U0024_04145) - 912434..913369 (+) 936 WP_304352048.1 ATP-binding protein -
  U0024_RS04150 (U0024_04150) - 913382..913852 (+) 471 WP_304352028.1 NUDIX domain-containing protein -
  U0024_RS04155 (U0024_04155) - 913865..914074 (-) 210 WP_034357520.1 DNA gyrase inhibitor YacG -
  U0024_RS04160 (U0024_04160) zapD 914174..914929 (-) 756 WP_304352029.1 cell division protein ZapD -
  U0024_RS04165 (U0024_04165) coaE 915017..915643 (-) 627 WP_304352030.1 dephospho-CoA kinase -
  U0024_RS04170 (U0024_04170) - 915647..916516 (-) 870 WP_304352031.1 prepilin peptidase -
  U0024_RS04175 (U0024_04175) pilC 916516..917733 (-) 1218 WP_304352032.1 type II secretion system F family protein Machinery gene
  U0024_RS04180 (U0024_04180) pilB 917758..919500 (-) 1743 WP_304352033.1 type IV-A pilus assembly ATPase PilB Machinery gene
  U0024_RS04190 (U0024_04190) - 919755..920684 (-) 930 WP_302092186.1 polyprenyl synthetase family protein -
  U0024_RS04195 (U0024_04195) rplU 921050..921361 (+) 312 WP_003058536.1 50S ribosomal protein L21 -
  U0024_RS04200 (U0024_04200) rpmA 921378..921635 (+) 258 WP_003058534.1 50S ribosomal protein L27 -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44568.40 Da        Isoelectric Point: 9.6093

>NTDB_id=912300 U0024_RS04175 WP_304352032.1 916516..917733(-) (pilC) [Comamonas testosteroni strain HAMBI_0403]
MASAASKGIKEFLFEWEGKDRNGKIVRGETRAGGENQIQAMLRRQGVTPSKIKKRRTRGGKKIKPKDIALFTRQLATMMK
AGVPLLQSFDIVGRGNTNPNVTKLLNDIRSDVETGTSLSAAFRKFPLYFNNLYCNLVEAGEAAGILESLLDRLATYMEKT
EAIKSKIKSALMYPISVMIVAFVVVTVIMIFVIPAFKEVFTSFGADLPAPTLLVMAISEYFVQYWWLIFGVLGGGLYFFM
QAWKRNERVQQFMDRAILKLPIFGVLIEKSCVARWTRTLSTMFAAGVPLVEALDSVGGASGNYLYKNATDRIQSEVSTGT
SLTVAMANANIFPSMVLQMCAIGEESGAIDHMLGKAADFYESEVDEMVAGLSSLMEPIIIVFLGTLIGGIVVSMYLPIFK
MGQVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=912300 U0024_RS04175 WP_304352032.1 916516..917733(-) (pilC) [Comamonas testosteroni strain HAMBI_0403]
ATGGCAAGCGCAGCGTCCAAGGGAATCAAGGAATTTCTCTTCGAGTGGGAAGGTAAGGACCGCAACGGCAAGATCGTTCG
CGGCGAGACTCGTGCCGGTGGAGAAAATCAGATCCAGGCCATGCTGCGCCGCCAGGGCGTGACTCCGTCCAAAATCAAGA
AGCGCAGGACCCGCGGCGGCAAGAAGATCAAGCCCAAGGACATTGCGCTGTTCACGCGCCAGCTCGCCACCATGATGAAG
GCCGGCGTGCCGCTGCTGCAGTCTTTCGACATCGTGGGCCGAGGCAACACCAACCCCAATGTCACCAAGCTGCTCAACGA
CATCCGCTCAGACGTGGAAACCGGCACCTCGCTGAGCGCGGCCTTTCGCAAGTTTCCACTCTATTTCAACAACCTCTACT
GCAACCTGGTGGAGGCCGGCGAGGCCGCAGGTATTCTGGAATCGCTGCTGGACCGTCTTGCCACCTACATGGAAAAGACG
GAAGCCATCAAGTCCAAGATCAAGTCCGCGCTGATGTATCCGATATCGGTGATGATCGTGGCCTTTGTCGTGGTGACCGT
GATCATGATCTTCGTGATCCCGGCCTTCAAGGAGGTCTTCACCTCCTTTGGCGCCGACCTGCCCGCGCCCACCTTGCTGG
TGATGGCAATCAGTGAGTATTTCGTACAGTACTGGTGGCTGATCTTCGGCGTGCTGGGTGGCGGCCTCTATTTCTTCATG
CAGGCCTGGAAGCGTAACGAGCGCGTGCAGCAGTTCATGGACCGCGCCATACTCAAGCTGCCTATCTTCGGCGTGCTGAT
TGAAAAGTCCTGCGTGGCCCGCTGGACGCGTACGCTGTCCACCATGTTTGCCGCCGGCGTGCCGCTGGTCGAGGCGCTGG
ACTCCGTGGGAGGCGCCTCGGGCAACTACCTCTACAAAAACGCCACCGACAGGATTCAGTCGGAAGTCTCCACAGGCACC
AGCCTCACGGTTGCCATGGCCAATGCCAATATCTTCCCTTCCATGGTGCTGCAGATGTGTGCCATCGGCGAGGAGTCGGG
CGCCATCGACCATATGCTGGGCAAGGCAGCCGATTTCTATGAGAGCGAAGTCGACGAAATGGTGGCGGGGCTGTCCAGCC
TGATGGAGCCCATCATCATCGTCTTCCTGGGCACCTTGATCGGCGGCATCGTGGTGTCCATGTATCTGCCTATCTTCAAG
ATGGGTCAAGTGGTGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

53.96

99.753

0.538
  pilG Neisseria gonorrhoeae MS11

51.134

98.025

0.501
  pilG Neisseria meningitidis 44/76-A

50.882

98.025

0.499
  pilC Legionella pneumophila strain ERS1305867

51.01

97.778

0.499
  pilC Acinetobacter baylyi ADP1

50

98.272

0.491
  pilC Acinetobacter baumannii D1279779

48.615

98.025

0.477
  pilC Vibrio cholerae strain A1552

40.05

98.025

0.393
  pilC Vibrio campbellii strain DS40M4

38.035

98.025

0.373
  pilC Thermus thermophilus HB27

36.634

99.753

0.365