Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   L6419_RS00790 Genome accession   NZ_AP025332
Coordinates   125820..126851 (+) Length   343 a.a.
NCBI ID   WP_272877595.1    Uniprot ID   -
Organism   Streptococcus ruminantium strain GUT-184     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 120820..131851
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6419_RS00775 (GUT184_01220) - 123431..123799 (+) 369 WP_155962601.1 DUF1033 family protein -
  L6419_RS00780 (GUT184_01230) - 123837..124787 (-) 951 WP_237375492.1 S66 family peptidase -
  L6419_RS00785 (GUT184_01240) comYA 124949..125899 (+) 951 WP_155962599.1 competence type IV pilus ATPase ComGA Machinery gene
  L6419_RS00790 (GUT184_01250) comYB 125820..126851 (+) 1032 WP_272877595.1 competence type IV pilus assembly protein ComGB Machinery gene
  L6419_RS00795 (GUT184_01260) comYC 126848..127129 (+) 282 WP_024532254.1 competence type IV pilus major pilin ComGC Machinery gene
  L6419_RS00800 (GUT184_01270) comGD 127116..127529 (+) 414 WP_024532253.1 competence type IV pilus minor pilin ComGD -
  L6419_RS00805 (GUT184_01280) comYE 127501..127794 (+) 294 WP_237375494.1 competence type IV pilus minor pilin ComGE Machinery gene
  L6419_RS00810 (GUT184_01290) comGF/cglF 127781..128215 (+) 435 WP_024532251.1 competence type IV pilus minor pilin ComGF Machinery gene
  L6419_RS00815 (GUT184_01300) comGG 128193..128606 (+) 414 WP_156011187.1 competence type IV pilus minor pilin ComGG -
  L6419_RS00820 (GUT184_01310) comYH 128611..129564 (+) 954 WP_237375496.1 class I SAM-dependent methyltransferase Machinery gene
  L6419_RS00825 (GUT184_01320) - 129614..130801 (+) 1188 WP_237375497.1 acetate kinase -
  L6419_RS00830 (GUT184_01330) - 131106..131660 (+) 555 WP_024532247.1 folate family ECF transporter S component -

Sequence

Protein


Download         Length: 343 a.a.        Molecular weight: 39026.95 Da        Isoelectric Point: 8.7768

>NTDB_id=91167 L6419_RS00790 WP_272877595.1 125820..126851(+) (comYB) [Streptococcus ruminantium strain GUT-184]
MIAFLQQDISVIGRQRQKRLSLDHQRKVIELFNNLFASGFHLGEIVDFLRRSQLLADRYTQVLSDGLLAGKPFSSLLADL
RFSDAVVTQVSLAEVHGNTSLSLFHIQSYLENVSKIRKKLIEVATYPVILLAFLLLIVVGLKNYLLPQLEEENIATVLIN
HLPAIFLSVVFLLSVGVLAGLTCYRKASKIKVFSRLVVLPFLGKVIQTYLTAYYAREWGSLIGQGLEMPQIVELMQTQRS
QLFREIGEDLESALSNGQSFHYHIQSYPFFKRELSLIIEYGQVKSKLGSELALYAAECWEEFFSRVNRAMQLIQPLVFLF
VALMVVLIYAAMLLPIYQNMELK

Nucleotide


Download         Length: 1032 bp        

>NTDB_id=91167 L6419_RS00790 WP_272877595.1 125820..126851(+) (comYB) [Streptococcus ruminantium strain GUT-184]
TTGATAGCCTTTTTGCAGCAGGATATATCAGTCATAGGCAGGCAGAGACAGAAAAGATTGTCTTTGGATCACCAGCGTAA
GGTGATTGAACTATTTAACAATCTCTTTGCAAGTGGCTTTCATTTGGGAGAAATTGTGGATTTTCTCAGGCGCAGTCAGC
TCCTGGCGGACCGTTACACACAGGTTTTATCAGATGGACTGTTGGCTGGTAAACCATTTTCTAGCCTTCTAGCGGATCTT
CGTTTTTCGGATGCTGTTGTCACTCAGGTTTCTCTTGCAGAAGTTCATGGTAATACTAGTTTGAGTCTGTTCCACATCCA
GTCCTATCTGGAAAATGTCAGTAAAATCCGTAAAAAATTGATCGAGGTTGCCACTTATCCAGTCATTTTACTGGCTTTTC
TTCTCCTGATTGTAGTAGGCTTGAAAAACTATCTCTTGCCGCAATTGGAAGAAGAAAACATAGCAACGGTGTTGATTAAC
CACTTACCAGCTATTTTTTTATCCGTAGTTTTCTTATTATCGGTAGGAGTTTTAGCAGGTCTTACTTGCTATCGCAAAGC
GAGCAAAATCAAGGTCTTTTCACGCTTGGTTGTTTTGCCATTTTTGGGAAAAGTTATCCAAACCTATCTAACAGCGTATT
ATGCTAGGGAGTGGGGAAGTCTAATCGGTCAGGGCTTAGAAATGCCACAGATTGTGGAGTTGATGCAGACACAGCGGTCG
CAATTATTCAGAGAGATTGGAGAAGATTTAGAATCAGCTCTTTCAAATGGTCAGAGCTTCCATTATCATATTCAGTCCTA
TCCATTTTTCAAGCGAGAGTTGAGTTTGATTATTGAGTATGGACAAGTCAAATCCAAACTGGGAAGCGAGTTGGCATTGT
ATGCAGCGGAGTGTTGGGAAGAATTTTTCTCCCGAGTCAATAGGGCCATGCAGTTGATTCAACCGCTAGTCTTTCTCTTT
GTAGCCTTGATGGTCGTTCTTATCTACGCAGCCATGTTGCTGCCTATTTATCAGAATATGGAGTTAAAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(30-147)

Type II secretion system (T2SS), protein F (T2SSF)

(215-335)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus gordonii str. Challis substr. CH1

63.158

99.708

0.63
  comGB/cglB Streptococcus mitis NCTC 12261

61.012

97.959

0.598
  comYB Streptococcus mutans UA159

58.43

100

0.586
  comYB Streptococcus mutans UA140

58.43

100

0.586
  comGB/cglB Streptococcus pneumoniae Rx1

59.821

97.959

0.586
  comGB/cglB Streptococcus pneumoniae R6

59.821

97.959

0.586
  comGB/cglB Streptococcus pneumoniae TIGR4

59.821

97.959

0.586
  comGB/cglB Streptococcus pneumoniae D39

59.821

97.959

0.586
  comGB/cglB Streptococcus mitis SK321

59.524

97.959

0.583
  comGB Lactococcus lactis subsp. cremoris KW2

49.852

98.251

0.49

Multiple sequence alignment