Detailed information NTG0091170

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: L6419_RS00810
• Genome accession: NZ_AP025332
• Coordinates: 127781..128215 (+)
• Length: 144 a.a.
• NCBI ID: WP_024532251.1
• Uniprot ID: -
• Organism: Streptococcus ruminantium strain GUT-184
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 122781..133215

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L6419_RS00775 (GUT184_01220)	-	123431..123799 (+)	369	WP_155962601.1	DUF1033 family protein	-
L6419_RS00780 (GUT184_01230)	-	123837..124787 (-)	951	WP_237375492.1	S66 family peptidase	-
L6419_RS00785 (GUT184_01240)	comYA	124949..125899 (+)	951	WP_155962599.1	competence type IV pilus ATPase ComGA	Machinery gene
L6419_RS00790 (GUT184_01250)	comYB	125820..126851 (+)	1032	WP_272877595.1	competence type IV pilus assembly protein ComGB	Machinery gene
L6419_RS00795 (GUT184_01260)	comYC	126848..127129 (+)	282	WP_024532254.1	competence type IV pilus major pilin ComGC	Machinery gene
L6419_RS00800 (GUT184_01270)	comGD	127116..127529 (+)	414	WP_024532253.1	competence type IV pilus minor pilin ComGD	-
L6419_RS00805 (GUT184_01280)	comYE	127501..127794 (+)	294	WP_237375494.1	competence type IV pilus minor pilin ComGE	Machinery gene
L6419_RS00810 (GUT184_01290)	comGF/cglF	127781..128215 (+)	435	WP_024532251.1	competence type IV pilus minor pilin ComGF	Machinery gene
L6419_RS00815 (GUT184_01300)	comGG	128193..128606 (+)	414	WP_156011187.1	competence type IV pilus minor pilin ComGG	-
L6419_RS00820 (GUT184_01310)	comYH	128611..129564 (+)	954	WP_237375496.1	class I SAM-dependent methyltransferase	Machinery gene
L6419_RS00825 (GUT184_01320)	-	129614..130801 (+)	1188	WP_237375497.1	acetate kinase	-
L6419_RS00830 (GUT184_01330)	-	131106..131660 (+)	555	WP_024532247.1	folate family ECF transporter S component	-
L6419_RS00835 (GUT184_01340)	-	131759..133015 (+)	1257	WP_237375498.1	bifunctional folylpolyglutamate synthase/dihydrofolate synthase	-

Sequence

Protein

• Length: 144 a.a.
• Molecular weight: 16473.95 Da
• Isoelectric Point: 8.8413

>NTDB_id=91170 L6419_RS00810 WP_024532251.1 127781..128215(+) (comGF/cglF) [Streptococcus ruminantium strain GUT-184]
MLKTRAPAFTLLECLVALVVLSGSLLVFEGLSKLISHEVRYQSKVLQKDWLLFSDQLRAEWAQAALVRVENNRIYINKEG
QGLAFGKSRSDDFRKTNEKGQGYQPMLYGLQATEIVQEGRLVRMDFTFTNGEERTFIYAFEKTG

Nucleotide

• Length: 435 bp

>NTDB_id=91170 L6419_RS00810 WP_024532251.1 127781..128215(+) (comGF/cglF) [Streptococcus ruminantium strain GUT-184]
TTGCTAAAAACTAGGGCTCCAGCTTTTACCCTTTTAGAATGTTTAGTGGCTTTGGTCGTCCTGTCGGGGAGTCTCTTAGT
ATTTGAAGGATTAAGCAAATTGATTTCTCATGAAGTTCGTTACCAAAGCAAGGTGCTTCAAAAGGATTGGCTCCTTTTCT
CGGATCAGCTGCGTGCAGAATGGGCTCAGGCGGCTTTGGTTAGAGTTGAGAATAACAGAATCTACATCAATAAGGAAGGT
CAAGGTCTTGCCTTTGGAAAATCACGTTCGGATGATTTTCGGAAGACGAATGAGAAGGGGCAAGGGTATCAGCCTATGTT
ATACGGTCTTCAAGCGACGGAAATTGTTCAAGAGGGAAGACTAGTTAGAATGGATTTTACCTTTACAAATGGAGAGGAGC
GAACCTTTATCTATGCTTTTGAAAAAACAGGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (40-134)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus mitis NCTC 12261	53.237	96.528	0.514
comGF/cglF	Streptococcus pneumoniae Rx1	52.899	95.833	0.507
comGF/cglF	Streptococcus pneumoniae D39	52.899	95.833	0.507
comGF/cglF	Streptococcus pneumoniae R6	52.899	95.833	0.507
comGF/cglF	Streptococcus pneumoniae TIGR4	52.899	95.833	0.507
comGF/cglF	Streptococcus mitis SK321	51.799	96.528	0.5
comYF	Streptococcus mutans UA159	50.355	97.917	0.493
comYF	Streptococcus mutans UA140	49.645	97.917	0.486
comGF	Lactococcus lactis subsp. cremoris KW2	45.775	98.611	0.451