Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   U0450_RS06285 Genome accession   NZ_CP139863
Coordinates   1258374..1259390 (+) Length   338 a.a.
NCBI ID   WP_078134945.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 15P2112     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1253374..1264390
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U0450_RS06265 nagA 1254521..1255672 (+) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  U0450_RS06270 - 1255835..1256893 (+) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  U0450_RS06275 - 1257044..1257409 (+) 366 WP_050212688.1 DUF1033 family protein -
  U0450_RS06280 comGA/cglA/cilD 1257485..1258426 (+) 942 WP_000249571.1 competence type IV pilus ATPase ComGA Machinery gene
  U0450_RS06285 comGB/cglB 1258374..1259390 (+) 1017 WP_078134945.1 competence type IV pilus assembly protein ComGB Machinery gene
  U0450_RS06290 comGC/cglC 1259392..1259718 (+) 327 WP_000738636.1 comG operon protein ComGC Machinery gene
  U0450_RS06295 comGD/cglD 1259711..1260115 (+) 405 WP_000588013.1 competence type IV pilus minor pilin ComGD Machinery gene
  U0450_RS06300 comGE/cglE 1260078..1260380 (+) 303 WP_226372546.1 competence type IV pilus minor pilin ComGE Machinery gene
  U0450_RS06305 comGF/cglF 1260343..1260804 (+) 462 WP_000250542.1 competence type IV pilus minor pilin ComGF Machinery gene
  U0450_RS06310 comGG/cglG 1260782..1261195 (+) 414 WP_000265624.1 competence type IV pilus minor pilin ComGG Machinery gene
  U0450_RS06315 - 1261332..1261926 (+) 595 Protein_1232 class I SAM-dependent methyltransferase -
  U0450_RS06320 comYH 1261987..1262940 (+) 954 WP_050233486.1 class I SAM-dependent methyltransferase Machinery gene
  U0450_RS06325 - 1262991..1264181 (+) 1191 WP_050233485.1 acetate kinase -
  U0450_RS06330 - 1264182..1264313 (+) 132 WP_000768904.1 hypothetical protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38424.39 Da        Isoelectric Point: 9.4802

>NTDB_id=911570 U0450_RS06285 WP_078134945.1 1258374..1259390(+) (comGB/cglB) [Streptococcus pneumoniae strain 15P2112]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=911570 U0450_RS06285 WP_078134945.1 1258374..1259390(+) (comGB/cglB) [Streptococcus pneumoniae strain 15P2112]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTTCCTTGTTGGATAAGCAGTGTG
TGACCCAGATGCGTACAGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTGCTATT
GTCACTCAGTTATCCCTAGCTGAAGTTCATGGCAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTGGACAATCT
GGCCAAGGTCAAGAAAAAATTGATTGAAGTAGCGACCTATCCCTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCAAATTATCGGTAATCTGCCACAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

99.112

100

0.991
  comGB/cglB Streptococcus pneumoniae D39

99.112

100

0.991
  comGB/cglB Streptococcus pneumoniae R6

99.112

100

0.991
  comGB/cglB Streptococcus pneumoniae TIGR4

99.112

100

0.991
  comGB/cglB Streptococcus mitis SK321

95.562

100

0.956
  comGB/cglB Streptococcus mitis NCTC 12261

94.675

100

0.947
  comYB Streptococcus gordonii str. Challis substr. CH1

71.131

99.408

0.707
  comYB Streptococcus mutans UA140

57.862

94.083

0.544
  comYB Streptococcus mutans UA159

57.862

94.083

0.544
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503