Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   U0450_RS06280 Genome accession   NZ_CP139863
Coordinates   1257485..1258426 (+) Length   313 a.a.
NCBI ID   WP_000249571.1    Uniprot ID   A0A0B7M3Q7
Organism   Streptococcus pneumoniae strain 15P2112     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1252485..1263426
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U0450_RS06260 - 1252551..1254368 (+) 1818 WP_001220920.1 acyltransferase family protein -
  U0450_RS06265 nagA 1254521..1255672 (+) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  U0450_RS06270 - 1255835..1256893 (+) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  U0450_RS06275 - 1257044..1257409 (+) 366 WP_050212688.1 DUF1033 family protein -
  U0450_RS06280 comGA/cglA/cilD 1257485..1258426 (+) 942 WP_000249571.1 competence type IV pilus ATPase ComGA Machinery gene
  U0450_RS06285 comGB/cglB 1258374..1259390 (+) 1017 WP_078134945.1 competence type IV pilus assembly protein ComGB Machinery gene
  U0450_RS06290 comGC/cglC 1259392..1259718 (+) 327 WP_000738636.1 comG operon protein ComGC Machinery gene
  U0450_RS06295 comGD/cglD 1259711..1260115 (+) 405 WP_000588013.1 competence type IV pilus minor pilin ComGD Machinery gene
  U0450_RS06300 comGE/cglE 1260078..1260380 (+) 303 WP_226372546.1 competence type IV pilus minor pilin ComGE Machinery gene
  U0450_RS06305 comGF/cglF 1260343..1260804 (+) 462 WP_000250542.1 competence type IV pilus minor pilin ComGF Machinery gene
  U0450_RS06310 comGG/cglG 1260782..1261195 (+) 414 WP_000265624.1 competence type IV pilus minor pilin ComGG Machinery gene
  U0450_RS06315 - 1261332..1261926 (+) 595 Protein_1232 class I SAM-dependent methyltransferase -
  U0450_RS06320 comYH 1261987..1262940 (+) 954 WP_050233486.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35591.51 Da        Isoelectric Point: 6.0083

>NTDB_id=911569 U0450_RS06280 WP_000249571.1 1257485..1258426(+) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 15P2112]
MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKMASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=911569 U0450_RS06280 WP_000249571.1 1257485..1258426(+) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 15P2112]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCAGCTCGGAAAAAAGGGACGCAGGATATCTATTTTGTCCCTAAGTT
AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATGGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACCTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B7M3Q7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae D39

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae R6

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

99.042

100

0.99
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

96.805

100

0.968
  comYA Streptococcus gordonii str. Challis substr. CH1

77.742

99.042

0.77
  comYA Streptococcus mutans UA159

65.916

99.361

0.655
  comYA Streptococcus mutans UA140

65.916

99.361

0.655
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.581

99.042

0.62
  comGA Lactococcus lactis subsp. cremoris KW2

54.808

99.681

0.546
  comGA Latilactobacillus sakei subsp. sakei 23K

42.642

84.665

0.361