Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PCI15_RS06645 Genome accession   NZ_AP025281
Coordinates   1458335..1460053 (-) Length   572 a.a.
NCBI ID   WP_271273550.1    Uniprot ID   -
Organism   Aliamphritea hakodatensis strain PT3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1453335..1465053
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCI15_RS06620 - 1454378..1455310 (+) 933 WP_271273546.1 Nudix family hydrolase -
  PCI15_RS06625 - 1455354..1455581 (-) 228 WP_336296733.1 DNA gyrase inhibitor YacG -
  PCI15_RS06630 coaE 1455598..1456194 (-) 597 WP_271273547.1 dephospho-CoA kinase -
  PCI15_RS06635 pilD 1456197..1457063 (-) 867 WP_271273548.1 A24 family peptidase Machinery gene
  PCI15_RS06640 pilC 1457088..1458314 (-) 1227 WP_271273549.1 type II secretion system F family protein Machinery gene
  PCI15_RS06645 pilB 1458335..1460053 (-) 1719 WP_271273550.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PCI15_RS06650 - 1460340..1460759 (+) 420 WP_271273551.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PCI15_RS06655 - 1460844..1461269 (+) 426 WP_271273552.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PCI15_RS06660 - 1461331..1461681 (+) 351 WP_271273553.1 hypothetical protein -
  PCI15_RS06665 nadC 1461755..1462606 (-) 852 WP_271273554.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PCI15_RS06670 ampD 1462799..1463335 (+) 537 WP_271273555.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PCI15_RS06675 - 1463349..1464416 (-) 1068 WP_271273556.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 63164.16 Da        Isoelectric Point: 5.0800

>NTDB_id=90994 PCI15_RS06645 WP_271273550.1 1458335..1460053(-) (pilB) [Aliamphritea hakodatensis strain PT3]
MSLQPFQPLSGLARRLVNDGVFSSDVAMEALKTSREEQTPFISHIIANRLVSAYRAAAAAADEFGMPILDLDMLDPESMP
KSLVDEKLISKHHALPLIQRENRLFVGISDPTNIQALDEFKFNTGITTEAVIVEEDKLNRLIDSFLDEQNSALEGLDDAD
LDNLDVEDPTENQQEDQADDAANDAPIVRFVNKILLDAIKNGASDIHFEPYEKSYRIRSRVDGILQEVAKPPTNLALQLS
ARLKVMSQMDISERRMPQDGRIKMRLSKKRSIDFRVNTLPTLWGEKIVLRILDPTSAQMGVEALGFEPEQKALYLETLEK
PQGMILVTGPTGSGKTVSLYTGLNILNTTERNISTAEDPVEINLEGINQVHVNTKVGLTFAEALRAFLRQDPDVVMVGEI
RDLETAEIGIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVPAFNIATSVSLIIAQRLARRLCTHCKQPANIPKEALKEAG
FSDEQLTNQLSLFEANPKGCGKCNKGYKGRVGIYEMLSMSQEIAESIMSNGSSLDIQAVAVKQGFKTLRHSGLHKVSQGL
TSLEEINRVIKV

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=90994 PCI15_RS06645 WP_271273550.1 1458335..1460053(-) (pilB) [Aliamphritea hakodatensis strain PT3]
ATGAGTTTACAGCCTTTTCAACCCTTAAGCGGACTTGCCAGACGGCTGGTCAATGACGGTGTTTTTTCTTCAGATGTTGC
CATGGAGGCACTTAAAACATCCCGTGAAGAGCAAACCCCGTTTATCTCCCATATTATTGCTAACCGTCTGGTCAGTGCTT
ACCGCGCAGCTGCCGCTGCCGCGGATGAGTTTGGCATGCCGATTCTGGATCTGGATATGCTCGATCCGGAAAGCATGCCT
AAAAGCCTGGTTGATGAGAAACTCATCAGTAAACATCACGCCCTGCCGCTGATACAACGGGAGAATCGCTTATTTGTCGG
CATCTCAGACCCGACCAATATACAGGCCCTAGATGAATTCAAGTTTAATACCGGTATAACCACCGAAGCGGTAATAGTTG
AAGAAGACAAACTAAACCGCCTGATCGACAGTTTTCTTGACGAACAAAACTCTGCTCTGGAAGGCCTGGACGATGCTGAT
CTGGACAATCTGGACGTCGAAGATCCAACTGAAAATCAACAGGAGGATCAGGCCGACGATGCAGCTAATGATGCGCCAAT
TGTTCGTTTCGTTAATAAAATTCTGTTAGATGCCATTAAAAATGGCGCATCCGATATTCACTTTGAACCGTACGAAAAAA
GCTACCGGATTCGTAGTCGGGTAGATGGCATTTTACAGGAAGTGGCTAAACCACCTACCAACTTAGCGCTGCAGCTGTCT
GCCCGTTTAAAAGTTATGTCCCAGATGGACATTTCTGAACGACGCATGCCTCAGGACGGGCGGATTAAAATGCGCCTGTC
GAAAAAACGCTCCATCGATTTCCGGGTAAACACCCTGCCCACCTTATGGGGTGAAAAGATTGTATTGCGTATTCTGGACC
CCACCAGCGCGCAGATGGGCGTAGAAGCCCTCGGATTCGAACCGGAACAGAAGGCTCTGTATCTGGAAACGTTAGAAAAA
CCCCAGGGTATGATTTTGGTCACCGGCCCCACCGGCAGCGGTAAAACCGTTTCCCTCTATACCGGCCTGAACATTCTTAA
CACTACCGAACGCAATATTTCCACCGCAGAAGATCCGGTGGAGATTAACCTCGAAGGCATCAACCAGGTGCATGTAAACA
CTAAGGTAGGTCTGACATTTGCTGAAGCCTTAAGGGCTTTCCTCCGTCAGGACCCGGATGTAGTCATGGTGGGTGAGATC
CGGGATCTGGAAACCGCCGAAATTGGCATTAAAGCCGCACAAACCGGCCACATGGTGCTCTCCACCCTGCACACCAACAG
CGCCCCGGAAACCCTGACCCGACTGCGCAACATGGGGGTGCCGGCATTTAACATAGCCACCTCCGTCAGTCTGATTATTG
CCCAGCGGCTGGCAAGACGCCTTTGTACCCACTGTAAACAGCCGGCAAACATCCCAAAAGAGGCACTAAAAGAAGCAGGC
TTTAGTGATGAGCAGCTCACTAATCAGTTAAGCTTGTTTGAAGCTAACCCTAAGGGCTGTGGTAAGTGCAACAAAGGATA
CAAAGGACGGGTCGGTATTTACGAAATGCTGTCTATGAGTCAGGAAATAGCCGAAAGCATTATGTCCAACGGCTCTTCAT
TAGATATTCAGGCGGTTGCTGTAAAACAGGGATTTAAAACCTTACGCCATTCCGGGTTACACAAAGTAAGTCAGGGCCTC
ACCAGCCTTGAAGAAATCAACCGGGTAATCAAGGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)

Type II/IV secretion system protein (T2SSE)

(193-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.22

98.601

0.584
  pilB Acinetobacter baumannii D1279779

59.075

98.252

0.58
  pilB Legionella pneumophila strain ERS1305867

54.85

99.126

0.544
  pilB Vibrio cholerae strain A1552

54.883

89.51

0.491
  pilF Neisseria gonorrhoeae MS11

49.464

97.902

0.484
  pilB Vibrio parahaemolyticus RIMD 2210633

53.516

89.51

0.479
  pilB Vibrio campbellii strain DS40M4

52.25

89.336

0.467
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.436

100

0.404
  pilB/pilB1 Synechocystis sp. PCC 6803

42.857

89.336

0.383
  pilF Thermus thermophilus HB27

36.655

98.252

0.36

Multiple sequence alignment