Detailed information NTG0909450

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: SMG00_RS20710
• Genome accession: NZ_CP139417
• Coordinates: 4439403..4440548 (-)
• Length: 381 a.a.
• NCBI ID: WP_020305662.1
• Uniprot ID: -
• Organism: Stutzerimonas stutzeri strain H46
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4434403..4445548

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SMG00_RS20685	-	4434480..4435496 (+)	1017	WP_011914964.1	aspartate carbamoyltransferase catalytic subunit	-
SMG00_RS20690	-	4435496..4436770 (+)	1275	WP_053528279.1	dihydroorotase	-
SMG00_RS20695	-	4436828..4437223 (-)	396	WP_011914966.1	TM2 domain-containing protein	-
SMG00_RS20700	-	4437359..4438573 (+)	1215	WP_053528278.1	putative DNA modification/repair radical SAM protein	-
SMG00_RS20705	-	4438573..4439406 (+)	834	WP_320893108.1	TIGR03915 family putative DNA repair protein	-
SMG00_RS20710	pilU	4439403..4440548 (-)	1146	WP_020305662.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
SMG00_RS20715	pilT	4440608..4441642 (-)	1035	WP_125872068.1	type IV pilus twitching motility protein PilT	Machinery gene
SMG00_RS20720	-	4441708..4442397 (+)	690	WP_320893109.1	YggS family pyridoxal phosphate-dependent enzyme	-
SMG00_RS20725	proC	4442408..4443232 (+)	825	WP_320893110.1	pyrroline-5-carboxylate reductase	-
SMG00_RS20730	-	4443242..4443832 (+)	591	WP_014598006.1	YggT family protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42538.82 Da
• Isoelectric Point: 6.8858

>NTDB_id=909450 SMG00_RS20710 WP_020305662.1 4439403..4440548(-) (pilU) [Stutzerimonas stutzeri strain H46]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVSKTPMSPEMTRETVHGVMNEQQRREFTENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTIEELKLPDVLKKLSMTKRGLVLFVGATGTGKSTSLASMIGYRNKNSSGHIISIED
PIEFIHQHQNCIVTQREVGIDTSSFEVALKNTLRQAPDVILIGEIRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPPDRHNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKSLMKRSTELGMQTFDQA
LYNLYAQGEITYEDALLHADSANDLRLLIKLGSETDGEHLTSVSQGLSLEVSDEDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=909450 SMG00_RS20710 WP_020305662.1 4439403..4440548(-) (pilU) [Stutzerimonas stutzeri strain H46]
ATGGAATTCGAGAAACTTCTGCGCCTGATGGTCGAAAAAGGCGGCTCCGATCTGTTCATCACGGCTGGCGTGCCGCCGTC
GATGAAGGTCAACGGCAAGATCATGCCGGTGAGCAAGACGCCCATGTCGCCGGAGATGACCCGGGAAACCGTGCACGGCG
TGATGAACGAGCAGCAGCGCCGCGAGTTCACCGAGAACCACGAATGCAACTTCGCCATCAGTGCCCGCGGCATCGGTCGC
TTCCGTGTCAGCGCGTTCTATCAGCGAAACCTGGCCGGCATGGTGCTGCGTCGCATCGAGACCAACATCCCGACCATCGA
AGAACTCAAGCTGCCGGACGTCCTGAAGAAGCTGTCGATGACCAAACGTGGTCTGGTGCTGTTCGTCGGTGCCACCGGTA
CCGGCAAGTCGACATCGCTGGCCTCGATGATCGGCTACCGCAACAAGAACTCCAGCGGGCACATCATCTCCATCGAAGAC
CCGATCGAATTCATTCACCAGCACCAGAATTGCATCGTCACCCAGCGCGAAGTGGGCATCGACACCAGCTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTCATCGGCGAGATCCGTACCCGCGAAACCATGGATTACG
CGGTCGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGCCGGACCGGCACAACCAGGTCTGGATGGACCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGACGCCTGACGGCAAGGGCCGTCGTGCGGTGATCGAGGTGCTGATCAATACGCCGCTGGCGGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAATCGCTGATGAAGCGCTCCACCGAGCTGGGTATGCAGACCTTCGACCAGGCG
CTGTACAACCTCTACGCGCAGGGGGAGATCACCTACGAAGATGCCCTGCTGCATGCTGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGGGTTCGGAAACCGACGGTGAGCACCTGACCAGCGTATCCCAGGGCCTGAGCCTGGAGGTCAGCGACG
AGGACCCGGGCCGGCGCTTCCGTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	94.751	100	0.948
pilU	Acinetobacter baylyi ADP1	66.106	93.701	0.619
pilU	Vibrio cholerae strain A1552	59.143	91.864	0.543
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.953	88.976	0.391
pilT	Pseudomonas aeruginosa PAK	41.194	87.927	0.362
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	40.828	88.714	0.362
pilT	Vibrio cholerae strain A1552	40.828	88.714	0.362