Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   L6424_RS02635 Genome accession   NZ_AP025277
Coordinates   565476..567182 (-) Length   568 a.a.
NCBI ID   WP_029302067.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain NUITM-VA1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 560476..572182
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6424_RS02605 (NUITMVA1_04990) mutT 561152..561565 (+) 414 WP_016352173.1 8-oxo-dGTP diphosphatase MutT -
  L6424_RS02610 (NUITMVA1_05000) yacG 561626..561820 (-) 195 WP_024945432.1 DNA gyrase inhibitor YacG -
  L6424_RS02615 (NUITMVA1_05010) zapD 561830..562552 (-) 723 WP_024945431.1 cell division protein ZapD -
  L6424_RS02620 (NUITMVA1_05020) coaE 562590..563204 (-) 615 WP_024945430.1 dephospho-CoA kinase -
  L6424_RS02625 (NUITMVA1_05030) pilD 563224..564096 (-) 873 WP_024945429.1 prepilin peptidase Machinery gene
  L6424_RS02630 (NUITMVA1_05040) pilC 564119..565360 (-) 1242 WP_011707570.1 type II secretion system F family protein Machinery gene
  L6424_RS02635 (NUITMVA1_05050) pilB 565476..567182 (-) 1707 WP_029302067.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  L6424_RS02640 (NUITMVA1_05060) - 567186..567608 (-) 423 WP_024944770.1 pilin -
  L6424_RS02645 (NUITMVA1_05070) nadC 567931..568788 (-) 858 WP_024944771.1 carboxylating nicotinate-nucleotide diphosphorylase -
  L6424_RS02650 (NUITMVA1_05080) - 568794..569264 (-) 471 WP_024944772.1 TIGR02281 family clan AA aspartic protease -
  L6424_RS02655 (NUITMVA1_05090) ampD 569411..569983 (+) 573 WP_024944773.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  L6424_RS02660 (NUITMVA1_05100) pdhR 570259..571023 (+) 765 WP_011707564.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62511.90 Da        Isoelectric Point: 5.4342

>NTDB_id=90934 L6424_RS02635 WP_029302067.1 565476..567182(-) (pilB) [Aeromonas hydrophila strain NUITM-VA1]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTTAIGKLMESDQDALGMEDIDESEI
SELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=90934 L6424_RS02635 WP_029302067.1 565476..567182(-) (pilB) [Aeromonas hydrophila strain NUITM-VA1]
ATGACCTCTAGCCCCAATAGCGGCCTGGCCTTAAGCCTGGCCGCCTCTTCCCTGCTCAGTGAGTCCGACTCACAACGTTA
TCTGAGCCAAGCCAAGGCGCAGCGTAAACCCTTTGTGACCTTCCTGATCGAAAATGAAATTCTCGACAGCAAAGCATTGG
CGGACTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAATAC
CTCAATCAGAAGCTGATTGAAAAACATCATGTCCTGCCCATTTACACCCAGGGGCACACCCTCTATATCGCCATGTCGGA
TCCCACCAATGTCTCTGCACTGGAGGATTTTGGTTTCAGCTTCGGGTTGCATACCGAGGCGCTGCTGGTCGAGGAGAACA
AACTCACCACAGCCATCGGCAAGCTGATGGAGAGCGATCAGGATGCCCTCGGCATGGAGGATATCGACGAATCCGAGATC
TCCGAACTGGAGGTCTCGGACGAGGGATCGCGGCTCGACGAGAGCGTCAATACAGCCGACGATGACGCGCCGATCGTCAA
ATACATCAACAAAATCATGATGGATGCCATCAAACGCGGGGCCTCCGACCTTCACTTCGAGCCCTATGAGACCAAATACC
GCATCCGCTTTCGGATAGACGGGATCTTGCATGAGATAGCAACTCCACCAGTCAATCTGGCCAACCGCTTCTCGGCCCGC
CTCAAGGTGATGGCACGGCTCGATATCGCCGAGCGGCGACTACCCCAGGATGGTCGCATCAAACTGAAGCTGTCGCGCAA
CAAATCGATGGACATGCGGGTCAATACCCTGCCCACCATGTGGGGCGAAAAAATCGTTATCCGTCTGCTCGACTCCTCCG
CCGCACGCCTCAATATCGAGCAGCTCGGTTTTGACGATCGCCAGAAGGCGCAATACCTGCGTGCCCTCTCGAAGCCTCAG
GGCATGATCCTGGTGACCGGCCCTACCGGTTCGGGCAAGACGGTTTCACTCTATACCGGCCTCAATATTCTCAATACCAC
CGAGGTCAATATCTCCACCGCCGAAGATCCGGTGGAGATCAACCTGCCCGGCGTCAACCAGGTGCAGGTTAATCCCAAGG
CAGGACTGACCTTCGCGAGCGCCCTGCGTTCTTTCTTGCGCCAAGACCCTGATGTGGTCATGGTGGGTGAAATCCGGGAT
CTGGAAACAGCCGAAATTGCCATCAAGGCCGCCCAGACCGGCCACCTGGTGCTATCCACTCTGCACACCAACTCGGCGGC
AGAAACGCTAACCCGGATGATGAACATGGGGGTACCCGCCTTCAATATCGCCTCCTCGGTCACCCTGATCATGGCTCAGC
GGCTGGCGCGTAAACTGTGCGATCACTGCAAAGCTCCCGAAGTGGTGCCCGAGGCTGAACTGCTTGAGCTGGGATTTACC
CAGCAGCAGCTGGCCGCAGGCTTGCGGCTGTTCAAACCGGTTGGCTGCAAAGAGTGCTCGGGTGGCTACAAGGGCCGGGT
CGGCATCTACGAAATCATGCTAATGTCTGAAAACATCGCAAAATTGATCATGCAGGGGGCCAACTCTCTGCAAATTGCTG
CCATCGCACAGAAGGAAGGCATGCGTACCTTGCGCACCTCCGGCCTGGAGAAGGCCCGCCTGGGGGTGACCAGTCTGGCG
GAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.712

100

0.558
  pilB Acinetobacter baylyi ADP1

55.439

100

0.556
  pilB Legionella pneumophila strain ERS1305867

54.336

99.472

0.54
  pilB Vibrio cholerae strain A1552

54.159

99.472

0.539
  pilB Vibrio campbellii strain DS40M4

51.943

99.648

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.423

98.944

0.509
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.152

92.958

0.373

Multiple sequence alignment