Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   SOI76_RS16875 Genome accession   NZ_CP139249
Coordinates   3527449..3529161 (+) Length   570 a.a.
NCBI ID   WP_104080648.1    Uniprot ID   -
Organism   Acinetobacter pittii strain MCR8900     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3522449..3534161
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SOI76_RS16840 (SOI76_16840) nusA 3523338..3524822 (-) 1485 WP_002115134.1 transcription termination factor NusA -
  SOI76_RS16845 (SOI76_16845) rimP 3524859..3525383 (-) 525 WP_016142372.1 ribosome maturation factor RimP -
  SOI76_RS16865 (SOI76_16865) secG 3526021..3526350 (-) 330 WP_016142373.1 preprotein translocase subunit SecG -
  SOI76_RS16870 (SOI76_16870) tpiA 3526363..3527157 (-) 795 WP_104080647.1 triose-phosphate isomerase -
  SOI76_RS16875 (SOI76_16875) pilB 3527449..3529161 (+) 1713 WP_104080648.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SOI76_RS16880 (SOI76_16880) pilC 3529189..3530415 (+) 1227 WP_104080649.1 type II secretion system F family protein Machinery gene
  SOI76_RS16885 (SOI76_16885) pilD 3530415..3531275 (+) 861 WP_104080650.1 prepilin peptidase Machinery gene
  SOI76_RS16890 (SOI76_16890) coaE 3531277..3531873 (+) 597 WP_104080651.1 dephospho-CoA kinase -
  SOI76_RS16895 (SOI76_16895) - 3531870..3532784 (-) 915 WP_104080652.1 DMT family transporter -
  SOI76_RS16900 (SOI76_16900) rlmB 3532820..3533569 (-) 750 WP_016142380.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  SOI76_RS16905 (SOI76_16905) - 3533672..3533998 (-) 327 WP_004795031.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63625.05 Da        Isoelectric Point: 5.0932

>NTDB_id=908307 SOI76_RS16875 WP_104080648.1 3527449..3529161(+) (pilB) [Acinetobacter pittii strain MCR8900]
MSVIHTSPKFSGFFRRLVEEKHVSAATMQTALDAAKRAKRDTVAYLIEEVHLSPSLLAETISIEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRVLPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKILTQQFTEESSFEFSDEEF
DLDVDLEAPPSQEDEEETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFLEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKIPTDIPKQSLLEMGF
TEQDLTHPDFQVFQPVGCTECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=908307 SOI76_RS16875 WP_104080648.1 3527449..3529161(+) (pilB) [Acinetobacter pittii strain MCR8900]
ATGTCAGTCATACATACATCTCCAAAATTTTCGGGGTTTTTTCGACGATTAGTCGAAGAAAAACATGTGTCGGCAGCGAC
CATGCAAACAGCATTAGATGCAGCAAAAAGAGCTAAACGGGATACGGTTGCGTACCTGATTGAAGAGGTTCATCTCTCCC
CTTCTTTATTAGCTGAAACAATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGATGTTTACGATACCAGCCAAATT
CCCAAAGATTTGGTCGATCAGAAACTGATTTTAAAACATCGGGTCTTACCTCTTATACAAAGAGGGCAGATTTTATATGT
CGCGACCAGCAATCCAAGTAATATTGAAGCGATCGATGCTATTCGCTTTAATAGCAAGCTAATGGTTGAGCCAGTTATTG
TCGAGCACCATAAGCTTGAGAAAATTCTGACGCAGCAATTTACCGAAGAAAGCAGTTTCGAGTTTAGTGATGAAGAGTTT
GATCTTGATGTAGACCTTGAAGCTCCACCCTCACAAGAGGATGAGGAAGAAACACCTCAAGGCGATGAAGCACCTATTGT
TAAATATATTAATAAATTACTCATCGATGCGATTCGTATGGGAGCTTCAGACTTGCATTTTGAACCCTATGAAAAATCAT
ATCGGGTCCGTTATCGAGTCGATGGTGTATTGCGTCAGATTGCAAATCCTCCTTTGCAATTGGCTAACCGTTTAGCTTCA
CGCTTAAAAGTCATGTCCCAAATGGATATTTCCGAAAAGCGAGTCCCTCAAGATGGTCGTATTAAGCTCAAGCTATCGAA
ATCTAAAGCAATTGATTTTCGTGTGAACTCTCTTCCGACCTTATTTGGTGAAAAACTGGTTCTGCGTATTCTCGATCCAT
CTAGCGCGATGCTCGGAATTGATGCTTTAGGATATGAAGAAGATCAGAAAGCTCTTTTTTTAGAAGCACTCGATAAACCA
CAAGGTATGCTTCTAATTACAGGCCCTACAGGTTCCGGTAAAACGGTATCTTTATATACGGGCTTAAATATTTTAAATAC
TGAAAGCTCTAATATTTCCACTGCCGAAGATCCAGTTGAAATTAACCTTGAAGGAATTAATCAGGTTAACGTCAACCCAA
AAGTAGGTCTGACTTTTTCTGCTGCACTTAAATCATTTTTACGTCAAGATCCAGACATTATTATGGTCGGTGAGATTCGT
GATTTAGAAACGGCTGAAATTGCGATTAAAGCTGCGCAAACCGGTCACATGGTCATGTCCACACTGCATACCAATAGTGC
TCCAGAAACACTGACTCGCCTACGCAATATGGGAGTTCCATCCTTTAATATCGCAACATCAGTCAACTTGGTTATTGCAC
AGCGTTTAGCACGTCGGCTATGCCCGCAGTGTAAAATACCTACTGACATTCCTAAACAGAGCCTATTAGAAATGGGCTTT
ACCGAACAAGATCTGACTCATCCCGATTTTCAAGTTTTCCAGCCTGTCGGTTGTACTGAGTGTCGTGAAGGCTATAAAGG
TCGAGTAGGTATTTACGAGGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCTGCTTCTGAAAAACTGGGGTTTAATAATCTGCGTCGTTCAGGGTTAAAGAAAGTCATGCAAGGTGTTACTTCT
TTACAGGAAGTCAATCGCGTGACTAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

95.439

100

0.954
  pilB Acinetobacter baylyi ADP1

78.421

100

0.784
  pilB Legionella pneumophila strain ERS1305867

51.852

99.474

0.516
  pilF Neisseria gonorrhoeae MS11

49.822

98.596

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

51.509

92.982

0.479
  pilB Vibrio campbellii strain DS40M4

50.958

91.579

0.467
  pilB Vibrio cholerae strain A1552

50.482

91.053

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

35.668

100

0.384
  pilF Thermus thermophilus HB27

36.833

98.596

0.363