Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   R2H34_RS01965 Genome accession   NZ_CP137746
Coordinates   420428..422152 (-) Length   574 a.a.
NCBI ID   WP_318342398.1    Uniprot ID   -
Organism   Acinetobacter sp. 16     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 415428..427152
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R2H34_RS01935 - 415512..415838 (+) 327 WP_004958605.1 pyrimidine/purine nucleoside phosphorylase -
  R2H34_RS01940 rlmB 416028..416777 (+) 750 WP_151711193.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  R2H34_RS01945 - 416808..417719 (+) 912 WP_151711192.1 DMT family transporter -
  R2H34_RS01950 coaE 417716..418315 (-) 600 WP_151711191.1 dephospho-CoA kinase -
  R2H34_RS01955 pilD 418317..419177 (-) 861 WP_318342396.1 A24 family peptidase Machinery gene
  R2H34_RS01960 pilC 419177..420403 (-) 1227 WP_318342397.1 type II secretion system F family protein Machinery gene
  R2H34_RS01965 pilB 420428..422152 (-) 1725 WP_318342398.1 type IV-A pilus assembly ATPase PilB Machinery gene
  R2H34_RS01970 tpiA 422498..423289 (+) 792 WP_004958617.1 triose-phosphate isomerase -
  R2H34_RS01975 secG 423302..423631 (+) 330 WP_004916847.1 preprotein translocase subunit SecG -
  R2H34_RS01995 rimP 424254..424778 (+) 525 WP_004916844.1 ribosome maturation factor RimP -
  R2H34_RS02000 nusA 424816..426300 (+) 1485 WP_004916840.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63611.79 Da        Isoelectric Point: 5.1320

>NTDB_id=900492 R2H34_RS01965 WP_318342398.1 420428..422152(-) (pilB) [Acinetobacter sp. 16]
MSGIHTSPKFSGFIRRIVDDGHIAASNMQSAIDAAKKAQQDIVAHLIEHHRLSPLTIAESISLEFGEPLFDLAVFDTALL
LREITDSKLIQKHRVVPLIQRGQILYVATSNPTNIDALDAIRFNTKLNIEPVIVEHNKLEKLIDQQFGDSGSFDFGENDD
FDLDNVDVATTDTNNTTEDEDTNNQEESPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIANPPLQLAT
RLASRLKVMSQMDISEKRMPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIEALGYEDEQKELFMSA
LDKPQGMLLITGPTGSGKTVSLYTGLNILNTEDTNISTAEDPVEINLEGINQVNVNPKTGLTFSAALKAFLRQDPDVIMV
GEIRDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSSCKQPIDIPKQSLL
EMGFTEQDLTTPGFQIFQPVGCDACREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAAASEKAGFDNLRRSGLKKVMQ
GITSLQEVNRVTNE

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=900492 R2H34_RS01965 WP_318342398.1 420428..422152(-) (pilB) [Acinetobacter sp. 16]
ATGTCAGGAATACATACGTCTCCAAAATTTTCGGGGTTTATTCGACGCATAGTCGATGATGGTCACATTGCTGCATCAAA
TATGCAGAGTGCGATTGATGCTGCAAAAAAAGCACAACAAGATATTGTTGCTCACTTAATTGAACATCATCGTCTATCAC
CTCTGACAATTGCGGAAAGCATATCTTTAGAGTTTGGTGAGCCACTCTTTGATCTTGCTGTATTTGATACAGCTCTATTA
CTTCGTGAAATAACTGACAGCAAACTGATTCAAAAACATCGTGTTGTTCCACTCATTCAACGTGGTCAGATTTTGTATGT
TGCGACCAGCAACCCGACGAATATTGATGCATTAGACGCAATCAGGTTCAATACCAAATTAAATATCGAACCTGTGATTG
TTGAGCACAATAAACTCGAAAAACTGATTGATCAGCAATTTGGAGATAGCGGCAGTTTCGATTTTGGGGAAAATGACGAC
TTTGATTTAGATAATGTTGATGTTGCAACGACTGATACAAACAACACGACTGAGGATGAGGATACAAATAATCAAGAAGA
ATCTCCTATCGTAAAATATATCAACAAGTTATTGATTGATGCGATTCGCATGGGAGCCTCTGACTTACATTTTGAGCCTT
ATGAAAAAATGTATCGCGTGCGTTATCGTGTGGATGGTGTACTCAGACAAATTGCTAACCCACCATTACAACTCGCAACT
CGTTTAGCCTCGCGCTTAAAAGTCATGTCTCAAATGGACATTTCTGAAAAGCGTATGCCTCAAGATGGTCGTATCAAACT
AAAACTCTCTAAAACTAAAGCGATTGATTTTCGTGTTAACTCACTTCCAACTTTATTTGGAGAGAAACTGGTTCTTCGTA
TCTTAGATCCTTCAAGCGCAATGTTAGGTATTGAAGCCTTAGGCTATGAGGATGAACAAAAAGAACTCTTTATGAGTGCA
TTAGACAAGCCTCAGGGTATGCTTCTGATTACAGGTCCAACAGGCTCAGGGAAAACTGTATCGTTGTATACAGGTTTAAA
TATCTTAAATACAGAAGATACCAATATTTCGACCGCAGAAGATCCCGTCGAAATTAACTTAGAAGGTATTAACCAAGTTA
ACGTCAACCCTAAAACAGGTTTAACTTTCTCAGCAGCATTAAAAGCTTTTTTACGTCAAGATCCTGATGTCATCATGGTC
GGTGAGATTCGTGATTTGGAAACAGCTGAAATTGCAATTAAAGCAGCTCAAACAGGTCACATGGTGATGTCGACCCTGCA
TACCAATAGCGCTCCTGAAACACTCACACGATTACGCAATATGGGGGTTCCCTCTTTCAATATTGCAACCTCGGTTAATC
TCGTGATTGCTCAACGTTTAGCCCGCCGCCTATGTTCTTCATGCAAACAACCTATCGATATTCCTAAACAAAGCCTTCTA
GAAATGGGTTTCACCGAACAAGACTTAACCACCCCAGGTTTCCAAATATTTCAACCTGTAGGCTGTGATGCTTGTCGTGA
AGGATATAAAGGACGTGTCGGTATTTATGAAGTGATGAAAGTGACACCAGAGATTTCCAAGATTATTATGGAAGATGGGA
ATGCACTACAAATTGCAGCAGCATCTGAAAAGGCTGGTTTCGATAATTTACGTCGTTCTGGTTTAAAAAAGGTCATGCAA
GGGATCACCTCTTTGCAAGAAGTTAACCGAGTAACAAATGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(196-463)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.903

100

0.812
  pilB Acinetobacter baylyi ADP1

76.522

100

0.767
  pilB Legionella pneumophila strain ERS1305867

52.788

93.728

0.495
  pilB Vibrio parahaemolyticus RIMD 2210633

49.731

97.038

0.483
  pilF Neisseria gonorrhoeae MS11

50.554

94.425

0.477
  pilB Vibrio cholerae strain A1552

48.917

96.516

0.472
  pilB Vibrio campbellii strain DS40M4

50.87

90.07

0.458
  pilB/pilB1 Synechocystis sp. PCC 6803

33.824

100

0.361
  pilF Thermus thermophilus HB27

36.702

98.258

0.361