Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   R2H34_RS00035 Genome accession   NZ_CP137746
Coordinates   4845..6020 (-) Length   391 a.a.
NCBI ID   WP_004914541.1    Uniprot ID   S7Y0P3
Organism   Acinetobacter sp. 16     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1..11020
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R2H34_RS00010 rpmH 692..826 (+) 135 WP_000831329.1 50S ribosomal protein L34 -
  R2H34_RS00015 rnpA 855..1250 (+) 396 WP_004950005.1 ribonuclease P protein component -
  R2H34_RS00020 yidD 1260..1580 (+) 321 WP_004950001.1 membrane protein insertion efficiency factor YidD -
  R2H34_RS00025 yidC 1586..3343 (+) 1758 WP_151825921.1 membrane protein insertase YidC -
  R2H34_RS00030 mnmE 3434..4789 (+) 1356 WP_151711393.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE -
  R2H34_RS00035 pilU 4845..6020 (-) 1176 WP_004914541.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  R2H34_RS00040 - 6400..7098 (+) 699 WP_151730672.1 DsbC family protein -
  R2H34_RS00045 - 7148..7399 (-) 252 WP_004914533.1 GlsB/YeaQ/YmgE family stress response membrane protein -
  R2H34_RS00050 hpt 7628..8155 (-) 528 WP_004914531.1 hypoxanthine phosphoribosyltransferase -
  R2H34_RS00055 guaD 8372..9697 (-) 1326 WP_087540365.1 guanine deaminase -
  R2H34_RS00060 - 9883..10356 (+) 474 WP_004914520.1 hypothetical protein -

Sequence

Protein


Download         Length: 391 a.a.        Molecular weight: 44323.84 Da        Isoelectric Point: 6.3220

>NTDB_id=900480 R2H34_RS00035 WP_004914541.1 4845..6020(-) (pilU) [Acinetobacter sp. 16]
MYTTELLEEAKTFMFHMLTKVVEYGGSDLFITADFPPSIKLQGLMRPLGQQALTAEKTKLFAYSLMNDKQRLDFENDWEC
NFAINVPNVSRFRVNVFKQQLQIGMVIRTITSEIPTFQKLKLPESLKNVIMEKRGLVLVVGGTGSGKSTSLAAMIDHRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHTWHHALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQALDRIINFFPEERRNQLLMDLSSNMKAIISQRLVRTQDGQGRRAAIEILLNTPLIADNILKGQFHVLKEIMTKS
RELGMQTFDQALFDLYNEGAISYDEALRNADSVNELRLQIKLKSSRQDGMPVSTTSLNMVKEEEVEEQEEA

Nucleotide


Download         Length: 1176 bp        

>NTDB_id=900480 R2H34_RS00035 WP_004914541.1 4845..6020(-) (pilU) [Acinetobacter sp. 16]
ATGTATACCACTGAATTATTAGAAGAAGCTAAAACCTTTATGTTTCATATGCTCACTAAAGTAGTTGAGTATGGGGGATC
TGATTTATTTATTACCGCAGATTTCCCACCTAGCATTAAGCTACAGGGTTTGATGCGCCCTTTAGGACAACAAGCGCTTA
CCGCAGAAAAAACCAAGTTGTTTGCTTATAGTTTAATGAATGACAAACAACGTCTAGACTTCGAAAATGATTGGGAATGT
AACTTTGCCATTAATGTCCCGAATGTTTCACGATTTCGGGTCAATGTATTTAAACAACAATTACAAATAGGCATGGTGAT
TCGAACCATTACATCTGAAATTCCAACCTTTCAAAAACTTAAACTCCCAGAATCACTGAAAAATGTGATTATGGAAAAAC
GTGGTTTGGTACTGGTGGTTGGCGGAACAGGTTCAGGTAAATCAACCTCTTTAGCAGCCATGATTGATCATCGAAATGAG
AACTCTGCTGGACATATCATTACCGTTGAAGATCCTGTTGAATACGTCCATAAACACAAAAAATCCATGATCACACACCG
TGAAGTTGGAGTTGATTGTCATACATGGCATCACGCGTTGAAAAATACGCTACGCCAAGCACCAGATGTGATTTTAATTG
GTGAAATACGTGATACTGAAACGATGGAACATGCGATTGCTTTTGCTGAAACAGGGCATCTATGTTTGGGAACACTGCAC
GCCAACAATGCCAATCAGGCACTGGATCGTATTATTAACTTTTTCCCAGAGGAACGTCGCAATCAACTGTTGATGGATCT
CTCATCCAATATGAAAGCGATTATTTCTCAACGTCTCGTGCGAACTCAAGATGGACAAGGTCGTCGAGCTGCGATTGAAA
TCCTCCTCAATACACCATTAATCGCAGACAATATTTTGAAAGGGCAATTCCATGTGCTCAAAGAGATCATGACCAAATCT
CGCGAGTTAGGTATGCAGACCTTTGATCAGGCTTTGTTTGATCTATATAACGAAGGTGCAATTAGTTACGATGAAGCATT
GCGTAACGCCGACTCTGTCAATGAGTTACGCTTGCAAATTAAATTAAAAAGTTCACGCCAAGATGGGATGCCCGTTTCAA
CCACTTCACTGAACATGGTCAAAGAAGAGGAAGTTGAAGAGCAAGAAGAAGCTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB S7Y0P3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.197

93.606

0.545
  pilU Acinetobacter baylyi ADP1

55.62

88.747

0.494
  pilU Vibrio cholerae strain A1552

52.601

88.491

0.465
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.284

85.678

0.371
  pilT Acinetobacter nosocomialis M2

44.172

83.376

0.368
  pilT Acinetobacter baumannii D1279779

44.172

83.376

0.368
  pilT Acinetobacter baumannii strain A118

44.172

83.376

0.368
  pilT Acinetobacter baylyi ADP1

42.56

85.934

0.366