Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   R2E40_RS20330 Genome accession   NZ_CP137631
Coordinates   4365598..4367313 (+) Length   571 a.a.
NCBI ID   WP_318164258.1    Uniprot ID   -
Organism   Aeromonas sp. CD     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4360598..4372313
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R2E40_RS20305 (R2E40_20305) pdhR 4361706..4362470 (-) 765 WP_011707564.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  R2E40_RS20310 (R2E40_20310) ampD 4362746..4363318 (-) 573 WP_202003185.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  R2E40_RS20315 (R2E40_20315) - 4363466..4363936 (+) 471 WP_318164255.1 retropepsin-like aspartic protease -
  R2E40_RS20320 (R2E40_20320) nadC 4363942..4364799 (+) 858 WP_318164256.1 carboxylating nicotinate-nucleotide diphosphorylase -
  R2E40_RS20325 (R2E40_20325) tapA 4365122..4365604 (+) 483 WP_318164257.1 type IVa pilus major pilin TapA -
  R2E40_RS20330 (R2E40_20330) pilB 4365598..4367313 (+) 1716 WP_318164258.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  R2E40_RS20335 (R2E40_20335) pilC 4367429..4368670 (+) 1242 WP_286041689.1 type II secretion system F family protein Machinery gene
  R2E40_RS20340 (R2E40_20340) pilD 4368693..4369565 (+) 873 WP_065392462.1 prepilin peptidase Machinery gene
  R2E40_RS20345 (R2E40_20345) coaE 4369585..4370199 (+) 615 WP_318164259.1 dephospho-CoA kinase -
  R2E40_RS20350 (R2E40_20350) zapD 4370237..4370959 (+) 723 WP_024945431.1 cell division protein ZapD -
  R2E40_RS20355 (R2E40_20355) yacG 4370969..4371163 (+) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  R2E40_RS20360 (R2E40_20360) mutT 4371224..4371637 (-) 414 WP_016352173.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62896.44 Da        Isoelectric Point: 5.4342

>NTDB_id=899562 R2E40_RS20330 WP_318164258.1 4365598..4367313(+) (pilB) [Aeromonas sp. CD]
MLIMTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIP
QKYLNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTTAIGKLMENDQDALGMEDIDE
SEISELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRF
SARLKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALS
KPQGMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGE
IRDLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLEL
GFTQQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVT
SLAEINRVTTN

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=899562 R2E40_RS20330 WP_318164258.1 4365598..4367313(+) (pilB) [Aeromonas sp. CD]
GTGCTAATTATGACTTCTAGTCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCCCTGCTCAGTGAGTCCGACTC
ACAACGCTATCTGAGCCAAGCCAAGGCCCAGCGCAAGCCCTTTGTGACCTTCCTGATCGAAAATGAAATCCTCGACAGCA
AGGCATTGGCTGACTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCAGAGATCCCG
CAGAAATACCTCAATCAGAAGCTGATTGAAAAACATCACGTTCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGC
CATGTCGGATCCCACCAATGTGTCTGCACTGGAGGATTTTGGTTTCAGCTTCGGGTTGCATACCGAGGCGCTGCTGGTCG
AGGAAAACAAACTCACCACGGCTATCGGCAAGCTGATGGAGAACGATCAGGATGCCCTCGGCATGGAGGACATCGACGAA
TCCGAGATTTCCGAGCTGGAAGTGTCTGACGAGGGATCGCGGCTCGACGAGAGCGTTAATACGGCCGACGACGACGCGCC
CATCGTCAAATACATCAACAAAATCATGATGGATGCCATCAAGCGCGGAGCCTCCGACCTCCACTTCGAACCCTATGAGA
CCAAATATCGCATCCGCTTTCGGATAGACGGGATCCTGCATGAAATAGCGACCCCACCGGTCAACCTGGCCAACCGCTTC
TCCGCCCGCCTCAAGGTGATGGCACGGCTCGACATCGCCGAGCGGCGACTGCCCCAGGATGGTCGCATCAAGCTCAAGCT
GTCGCGCAACAAATCGATGGATATGCGGGTCAATACCCTGCCCACCATGTGGGGCGAAAAGATCGTTATCCGTCTGCTCG
ACTCCTCCGCTGCACGCCTCAATATCGAGCAGCTCGGTTTCGACGATCGGCAGAAGGCGCAATATCTGCGTGCTCTGTCG
AAGCCTCAGGGCATGATCCTGGTGACCGGCCCTACCGGTTCGGGCAAGACGGTTTCACTCTATACCGGTCTCAATATTCT
CAATACCACCGAGGTCAATATCTCCACCGCCGAAGACCCGGTGGAGATCAACCTGCCCGGCGTCAATCAGGTGCAGGTCA
ATCCCAAGGCAGGACTGACCTTCGCGAGCGCCCTGCGCTCTTTCTTGCGCCAAGACCCCGATGTGGTCATGGTGGGTGAA
ATCCGGGATCTGGAAACAGCCGAAATTGCCATCAAGGCCGCCCAGACCGGCCACCTGGTGCTATCCACCCTGCACACCAA
CTCGGCGGCGGAAACGCTGACCCGGATGATGAACATGGGAGTACCCGCCTTCAATATCGCCTCCTCGGTCACCCTGATCA
TGGCTCAGCGGCTGGCGCGTAAACTGTGCGATCACTGCAAAGCCCCCGAAGTGGTGCCAGAGGCTGAGCTGCTTGAGCTG
GGTTTTACCCAGCAGCAGCTGGCCGCTGGCTTGCGGCTGTTCAAACCGGTTGGCTGCAAAGAGTGCTCGGGTGGCTACAA
GGGCCGGGTCGGCATTTACGAAATCATGCTAATGTCTGAAAACATCGCAAAATTGATCATGCAGGGGGCCAACTCTCTGC
AAATTGCCGCCATTGCCCAGAAGGAGGGCATGCGCACCTTGCGCACCTCCGGCCTTGAGAAGGCCCGCCTGGGGGTGACC
AGTCTGGCGGAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.517

100

0.555
  pilB Acinetobacter baylyi ADP1

55.323

100

0.555
  pilB Legionella pneumophila strain ERS1305867

54.064

99.124

0.536
  pilB Vibrio cholerae strain A1552

53.982

98.949

0.534
  pilB Vibrio campbellii strain DS40M4

51.943

99.124

0.515
  pilB Vibrio parahaemolyticus RIMD 2210633

51.423

98.424

0.506
  pilF Neisseria gonorrhoeae MS11

46.454

98.774

0.459
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

96.848

0.377
  pilF Thermus thermophilus HB27

40.152

92.469

0.371