Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   R2E40_RS20340 Genome accession   NZ_CP137631
Coordinates   4368693..4369565 (+) Length   290 a.a.
NCBI ID   WP_065392462.1    Uniprot ID   -
Organism   Aeromonas sp. CD     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4363693..4374565
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R2E40_RS20320 (R2E40_20320) nadC 4363942..4364799 (+) 858 WP_318164256.1 carboxylating nicotinate-nucleotide diphosphorylase -
  R2E40_RS20325 (R2E40_20325) tapA 4365122..4365604 (+) 483 WP_318164257.1 type IVa pilus major pilin TapA -
  R2E40_RS20330 (R2E40_20330) pilB 4365598..4367313 (+) 1716 WP_318164258.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  R2E40_RS20335 (R2E40_20335) pilC 4367429..4368670 (+) 1242 WP_286041689.1 type II secretion system F family protein Machinery gene
  R2E40_RS20340 (R2E40_20340) pilD 4368693..4369565 (+) 873 WP_065392462.1 prepilin peptidase Machinery gene
  R2E40_RS20345 (R2E40_20345) coaE 4369585..4370199 (+) 615 WP_318164259.1 dephospho-CoA kinase -
  R2E40_RS20350 (R2E40_20350) zapD 4370237..4370959 (+) 723 WP_024945431.1 cell division protein ZapD -
  R2E40_RS20355 (R2E40_20355) yacG 4370969..4371163 (+) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  R2E40_RS20360 (R2E40_20360) mutT 4371224..4371637 (-) 414 WP_016352173.1 8-oxo-dGTP diphosphatase MutT -
  R2E40_RS20365 (R2E40_20365) - 4371646..4372830 (-) 1185 WP_318164260.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 32480.78 Da        Isoelectric Point: 6.7410

>NTDB_id=899564 R2E40_RS20340 WP_065392462.1 4368693..4369565(+) (pilD) [Aeromonas sp. CD]
MLLITDVFHSLPWLYFSLVFLFSLMIGSFLNVVIHRLPIMLEREWQAEYLGYFNPETQPQQEERYNLMVPRSACPHCGHA
ITAMENIPLLSWLWLKGRCRECQAPISARYPLVELLTALLSLVVAATFPPGWGLLAALLLTWVLVALTFIDLDKMLLPDQ
LTLPLLWGGLLFNLAGGFAPLADAVIGAMAGYLVLWSLYWAFKLLTGKEGMGYGDFKLLAALGAWLGWQALPIVLLLSSL
VGAFIGIGLILLRNHHQNKPIPFGPYLAIAGWIALLWGDTITRWYLTTFL

Nucleotide


Download         Length: 873 bp        

>NTDB_id=899564 R2E40_RS20340 WP_065392462.1 4368693..4369565(+) (pilD) [Aeromonas sp. CD]
ATGTTGCTGATCACTGACGTTTTTCACAGCCTGCCCTGGCTCTATTTCTCGCTGGTATTCCTCTTTTCTCTGATGATCGG
CAGTTTTCTCAACGTGGTCATTCATCGCCTGCCCATCATGCTGGAACGGGAGTGGCAGGCCGAATATCTCGGCTATTTCA
ATCCAGAGACACAGCCGCAGCAGGAAGAGCGCTACAACCTGATGGTGCCGCGTTCTGCCTGTCCCCATTGCGGCCACGCC
ATCACGGCCATGGAGAACATCCCCCTGCTCAGCTGGCTCTGGCTCAAGGGGCGCTGCCGTGAATGCCAGGCCCCCATCTC
GGCCCGTTATCCTCTGGTGGAGCTGCTGACGGCCCTGCTCTCGCTGGTCGTCGCCGCAACATTCCCACCGGGCTGGGGCT
TGCTGGCCGCCCTGCTGCTCACCTGGGTACTGGTGGCGCTCACTTTCATCGATCTGGACAAGATGCTGCTGCCTGACCAG
CTGACCCTGCCACTGCTGTGGGGCGGCCTGCTGTTCAACCTGGCAGGGGGATTTGCACCGCTGGCCGATGCGGTGATCGG
TGCCATGGCCGGCTATCTGGTGCTCTGGAGCCTCTACTGGGCCTTCAAGCTGCTGACCGGCAAGGAAGGGATGGGCTATG
GTGACTTCAAGCTGCTGGCGGCGCTGGGTGCTTGGCTGGGCTGGCAGGCACTGCCCATCGTGTTGCTGCTCTCGTCACTG
GTTGGCGCTTTCATCGGGATTGGTCTCATTCTGCTGCGCAACCATCATCAGAACAAACCCATCCCCTTCGGCCCCTATCT
GGCCATCGCTGGCTGGATCGCGCTGCTGTGGGGCGATACCATCACCCGCTGGTACCTCACGACGTTTCTCTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-128)

Type IV leader peptidase family (Peptidase_A24)

(139-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

61.672

98.966

0.61
  pilD Vibrio campbellii strain DS40M4

55.944

98.621

0.552
  pilD Acinetobacter baumannii D1279779

54.225

97.931

0.531
  pilD Acinetobacter nosocomialis M2

54.225

97.931

0.531
  pilD Neisseria gonorrhoeae MS11

48.718

94.138

0.459