Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   R2B70_RS02315 Genome accession   NZ_CP137630
Coordinates   489498..491204 (-) Length   568 a.a.
NCBI ID   WP_042077628.1    Uniprot ID   -
Organism   Aeromonas sp. XH     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 484498..496204
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R2B70_RS02285 (R2B70_02285) mutT 485123..485527 (+) 405 WP_318162532.1 8-oxo-dGTP diphosphatase MutT -
  R2B70_RS02290 (R2B70_02290) yacG 485586..485780 (-) 195 WP_042077620.1 DNA gyrase inhibitor YacG -
  R2B70_RS02295 (R2B70_02295) zapD 485793..486515 (-) 723 WP_209794190.1 cell division protein ZapD -
  R2B70_RS02300 (R2B70_02300) coaE 486553..487167 (-) 615 WP_318162533.1 dephospho-CoA kinase -
  R2B70_RS02305 (R2B70_02305) pilD 487186..488061 (-) 876 WP_318162534.1 A24 family peptidase Machinery gene
  R2B70_RS02310 (R2B70_02310) pilC 488129..489370 (-) 1242 WP_209794187.1 type II secretion system F family protein Machinery gene
  R2B70_RS02315 (R2B70_02315) pilB 489498..491204 (-) 1707 WP_042077628.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  R2B70_RS02320 (R2B70_02320) - 491208..491624 (-) 417 WP_318162535.1 pilin -
  R2B70_RS02325 (R2B70_02325) nadC 491934..492809 (-) 876 WP_318162536.1 carboxylating nicotinate-nucleotide diphosphorylase -
  R2B70_RS02330 (R2B70_02330) - 492813..493283 (-) 471 WP_042077632.1 TIGR02281 family clan AA aspartic protease -
  R2B70_RS02335 (R2B70_02335) ampD 493396..493965 (+) 570 WP_318162537.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  R2B70_RS02340 (R2B70_02340) pdhR 494244..495008 (+) 765 WP_010675815.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62633.01 Da        Isoelectric Point: 5.8214

>NTDB_id=899519 R2B70_RS02315 WP_042077628.1 489498..491204(-) (pilB) [Aeromonas sp. XH]
MTSSLNSGLATSLAASSLLSEPDSQRYLSQARAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEESKLTAAIGKLMESEQDTLGMDHIDEAEI
SELEVSDENSRLDESVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRNRSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIDQLGFDDRQKGQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTNEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSDNIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=899519 R2B70_RS02315 WP_042077628.1 489498..491204(-) (pilB) [Aeromonas sp. XH]
ATGACCTCTAGCCTTAATAGCGGCCTGGCCACCAGCCTGGCCGCTTCCTCCCTTCTCAGTGAGCCGGACTCACAGCGCTA
TCTGAGCCAGGCCAGGGCGCAGCGCAAGCCATTTGTCACCTTCCTGATTGAAAATGACATCCTCGACAGCAAGGCCCTCG
CCGACTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTGGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAATAC
CTCAATCAAAAGCTGATTGAAAAACATCACGTCCTGCCCATCTATACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
TCCGACCAACGTGTCGGCCCTGGAGGATTTCGGCTTCAGCTTCGGTCTGCACACGGAAGCCTTGCTGGTCGAGGAGAGCA
AGCTGACCGCCGCCATCGGCAAATTGATGGAAAGCGAGCAGGATACCCTCGGCATGGATCACATCGACGAGGCCGAGATC
TCCGAGCTCGAGGTGTCCGACGAGAACTCTCGTCTCGATGAGAGCGTCAATACGGCCGACGATGACGCCCCCATCGTCAA
GTACATCCACAAGATCATGATGGACGCCATCAAGCGCGGCGCATCGGACCTGCATTTCGAACCTTACGAGACCAAGTACC
GGATCCGCTTCCGGATCGACGGCATACTGCACGAAATCGCCACCCCGCCGGTGAACCTGGCAAACCGTTTCGCGGCGCGC
TTGAAAGTCATGGCACGGCTCGACATCGCCGAGCGGCGTCTGCCCCAGGATGGCCGCATCAAGCTCAAGATTTCGCGCAA
CAGATCCATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGCGAGAAGATCGTCATCCGACTTCTGGATTCATCTG
CAGCCAGGCTCAATATCGATCAACTCGGCTTCGACGACCGCCAGAAGGGGCAATACCTGCATGCCCTCTCCAAACCCCAG
GGGATGATACTGGTCACCGGCCCCACGGGGTCGGGCAAGACGGTATCGCTCTATACCGGTCTCAACATCCTCAATACCAA
CGAGGTCAACATCTCCACCGCCGAGGATCCGGTGGAGATCAACCTGCCCGGCGTGAACCAGGTGCAGATCAACCCCAAGG
CGGGGCTCACCTTCGCCAGCGCCCTGCGCTCCTTCCTGCGCCAGGATCCCGACATCGTCATGGTGGGGGAGATCCGGGAT
CTGGAAACCGCCGAGATCGCCATCAAGGCCGCCCAGACCGGCCACCTGGTGCTGTCCACCCTGCACACCAATTCGGCGGC
CGAGACGCTCACCCGGATGATGAACATGGGTGTCCCCGCCTTCAACATCGCCTCCTCGGTCACCCTCATCATGGCCCAGC
GCCTCGCCCGCAAGTTGTGCGATCACTGCAAGGCGCCCGAGGTGGTGCCGGAAGCCGAGCTGCTGGAGCTGGGCTTTACC
CAGCAGCAACTGGCCGCCGGCTTGCGACTGTTCAAACCCGTGGGGTGCAAGGAGTGCTCGGGAGGCTACCGAGGGCGGGT
CGGGATCTATGAGATCATGCTGATGTCAGACAACATCGCCAAGCTCATCATGCAAGGAGCCAACTCGCTGCAAATCGCGG
CCATTGCCCAGAAGGAAGGGATGCGCACCCTGCGCACCTCCGGCCTTGAAAAAGCCCGCCTGGGAGTCACCAGCCTGGCC
GAAATAAACAGGATCACTACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.85

99.824

0.548
  pilB Acinetobacter baylyi ADP1

54.885

99.12

0.544
  pilB Legionella pneumophila strain ERS1305867

54.513

99.472

0.542
  pilB Vibrio cholerae strain A1552

53.24

100

0.535
  pilB Vibrio campbellii strain DS40M4

50.53

99.648

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

49.911

99.12

0.495
  pilF Neisseria gonorrhoeae MS11

46.208

99.824

0.461
  pilF Thermus thermophilus HB27

40.341

92.958

0.375
  pilB/pilB1 Synechocystis sp. PCC 6803

37.681

97.183

0.366